Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200302166> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4200302166 endingPage "107059" @default.
- W4200302166 startingPage "107059" @default.
- W4200302166 abstract "As a class of non-coding RNA nucleotides, miRNAs has been widely regarded as an effective clinical biomarker for diagnosis and therapy of a variety of disease, such as colorectal cancer. However, development of an easy-to-operate and high-sensitive miRNA detection methods remains a huge challenge due to the low expression level, and high similarity with homologous RNA nucleotides. Herein, a novel CHA (catalytic hairpin assembly)-based sensing platform was established for sensitive, efficient and reliable in vitro detection of miRNA-21. The CHA based scaffold was constructed in a one-step self-assembly process of designed DNA probes (a S strand, a pair of hairpin probes). The presence of target miRNA initiates CHA process to form double-strand DNA (dsDNA) complex between the pair of hairpin probes, exposing nicking enzyme recognizing site. Nb.BbvCI identifies recognizing site in dsDNA section and generates a nick. With DNA polymerase based chain extension and replacement, liberated chain attends a next CHA process and a signal circle is formed. Compared with CHA, the method significant improves the sensitivity for miRNA-21 detection in vitro, showing a wider application for trace sample detection." @default.
- W4200302166 created "2021-12-31" @default.
- W4200302166 creator A5012167465 @default.
- W4200302166 creator A5016219123 @default.
- W4200302166 date "2022-03-01" @default.
- W4200302166 modified "2023-10-17" @default.
- W4200302166 title "Nicking site enzyme assisted catalytic hairpin assembly based scaffold for sensitive monitoring of miRNA-21" @default.
- W4200302166 cites W1968235736 @default.
- W4200302166 cites W2160210255 @default.
- W4200302166 cites W2470277042 @default.
- W4200302166 cites W2729786396 @default.
- W4200302166 cites W2753408816 @default.
- W4200302166 cites W2771980683 @default.
- W4200302166 cites W2775162699 @default.
- W4200302166 cites W2884720384 @default.
- W4200302166 cites W2888503917 @default.
- W4200302166 cites W2900597665 @default.
- W4200302166 cites W2911377111 @default.
- W4200302166 cites W2938163732 @default.
- W4200302166 cites W2973499684 @default.
- W4200302166 cites W2998639792 @default.
- W4200302166 cites W3037480077 @default.
- W4200302166 cites W3046086628 @default.
- W4200302166 cites W3092238661 @default.
- W4200302166 cites W3111550603 @default.
- W4200302166 cites W3114835926 @default.
- W4200302166 cites W3137883960 @default.
- W4200302166 doi "https://doi.org/10.1016/j.microc.2021.107059" @default.
- W4200302166 hasPublicationYear "2022" @default.
- W4200302166 type Work @default.
- W4200302166 citedByCount "2" @default.
- W4200302166 countsByYear W42003021662022 @default.
- W4200302166 countsByYear W42003021662023 @default.
- W4200302166 crossrefType "journal-article" @default.
- W4200302166 hasAuthorship W4200302166A5012167465 @default.
- W4200302166 hasAuthorship W4200302166A5016219123 @default.
- W4200302166 hasConcept C104317684 @default.
- W4200302166 hasConcept C145059251 @default.
- W4200302166 hasConcept C153911025 @default.
- W4200302166 hasConcept C185592680 @default.
- W4200302166 hasConcept C190232843 @default.
- W4200302166 hasConcept C512185932 @default.
- W4200302166 hasConcept C552990157 @default.
- W4200302166 hasConcept C55493867 @default.
- W4200302166 hasConcept C67705224 @default.
- W4200302166 hasConcept C70721500 @default.
- W4200302166 hasConcept C86803240 @default.
- W4200302166 hasConceptScore W4200302166C104317684 @default.
- W4200302166 hasConceptScore W4200302166C145059251 @default.
- W4200302166 hasConceptScore W4200302166C153911025 @default.
- W4200302166 hasConceptScore W4200302166C185592680 @default.
- W4200302166 hasConceptScore W4200302166C190232843 @default.
- W4200302166 hasConceptScore W4200302166C512185932 @default.
- W4200302166 hasConceptScore W4200302166C552990157 @default.
- W4200302166 hasConceptScore W4200302166C55493867 @default.
- W4200302166 hasConceptScore W4200302166C67705224 @default.
- W4200302166 hasConceptScore W4200302166C70721500 @default.
- W4200302166 hasConceptScore W4200302166C86803240 @default.
- W4200302166 hasLocation W42003021661 @default.
- W4200302166 hasOpenAccess W4200302166 @default.
- W4200302166 hasPrimaryLocation W42003021661 @default.
- W4200302166 hasRelatedWork W1657463452 @default.
- W4200302166 hasRelatedWork W1976736675 @default.
- W4200302166 hasRelatedWork W2002473487 @default.
- W4200302166 hasRelatedWork W2054652705 @default.
- W4200302166 hasRelatedWork W2088503398 @default.
- W4200302166 hasRelatedWork W2316086147 @default.
- W4200302166 hasRelatedWork W2387605231 @default.
- W4200302166 hasRelatedWork W2594860815 @default.
- W4200302166 hasRelatedWork W3117169687 @default.
- W4200302166 hasRelatedWork W3136905963 @default.
- W4200302166 hasVolume "174" @default.
- W4200302166 isParatext "false" @default.
- W4200302166 isRetracted "false" @default.
- W4200302166 workType "article" @default.