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- W4200308674 endingPage "13332" @default.
- W4200308674 startingPage "13332" @default.
- W4200308674 abstract "Proteins, lipids, and carbohydrates from the harmful algal bloom (HAB)-causing organism Pyrodinium bahamense were characterized to obtain insights into the biochemical processes in this environmentally relevant dinoflagellate. Shotgun proteomics using label-free quantitation followed by proteome mapping using the P. bahamense transcriptome and translated protein databases of Marinovum algicola, Alexandrium sp., Cylindrospermopsis raciborskii, and Symbiodinium kawagutii for annotation enabled the characterization of the proteins in P. bahamense. The highest number of annotated hits were obtained from M. algicola and highlighted the contribution of microorganisms associated with P. bahamense. Proteins involved in dimethylsulfoniopropionate (DMSP) degradation such as propionyl CoA synthethase and acryloyl-CoA reductase were identified, suggesting the DMSP cleavage pathway as the preferred route in this dinoflagellate. Most of the annotated proteins were involved in amino acid biosynthesis and carbohydrate degradation and metabolism, indicating the active roles of these molecules in the vegetative stage of P. bahamense. This characterization provides baseline information on the cellular machinery and the molecular basis of the ecophysiology of P. bahamense." @default.
- W4200308674 created "2021-12-31" @default.
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- W4200308674 creator A5089641524 @default.
- W4200308674 date "2021-12-11" @default.
- W4200308674 modified "2023-09-25" @default.
- W4200308674 title "Biochemical Mapping of Pyrodinium bahamense Unveils Molecular Underpinnings behind Organismal Processes" @default.
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