Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200311172> ?p ?o ?g. }
- W4200311172 abstract "MicroRNA156 (miR156) is an important conserved miRNA family in plants. Recently, we revealed VvmiR156a could involve in the modulation of gibberellin (GA)-mediated flower and berry development process of grapevine (Vitis vinifera L.). However, how to manipulate this process is unclear. For this, we used the GA-induced grapevine parthenocarpy system to investigate the regulatory roles of VvmiR156a during this process. Here, we cloned the mature and precursor sequences of VvmiR156a in Wink grape and identified its potential target gene VvAGL80, which belongs to the MADS-box gene family. Moreover, using RNA ligase-mediated 5' rapid amplification of cDNA ends (RLM-RACE) and poly(A)polymerase-mediated 3' rapid amplification of cDNA (PPM-RACE) technologies, it confirmed that VvAGL80 was the true target gene of VvmiR159a. Analysis of promoter cis-elements and β-glucuronidase (GUS) staining showed that both VvmiR156a and VvAGL80 contained GA-responsive elements and could respond to GA treatments. Quantitative real-time-polymerase chain reaction (qRT-PCR) analysis exhibited the VvmiR156a and VvAGL80 showed opposite expression trends during grapevine flower and berry development, indicating that VvmiR156a negatively regulated the expression of VvAGL80 during this process. After GA treatment, the expression of miR156 in flowers was downregulated significantly, while that of VvAGL80 was upregulated, thereby accelerating grapevine flowering. Furthermore, GA treatment enhanced the negative regulation of VvmiR156a on VvAGL80 in seed, especially at the seed-coat hardening stage, which was the key period of seed growth and development. Our findings enriched the knowledge of the regulatory mechanism of the miRNA-mediated grapevine parthenocarpy process." @default.
- W4200311172 created "2021-12-31" @default.
- W4200311172 creator A5013038112 @default.
- W4200311172 creator A5018688675 @default.
- W4200311172 creator A5026199531 @default.
- W4200311172 creator A5030246181 @default.
- W4200311172 creator A5035694858 @default.
- W4200311172 creator A5058171957 @default.
- W4200311172 creator A5064311978 @default.
- W4200311172 creator A5065495869 @default.
- W4200311172 creator A5072537482 @default.
- W4200311172 creator A5075086498 @default.
- W4200311172 creator A5085695020 @default.
- W4200311172 creator A5086529957 @default.
- W4200311172 date "2021-12-09" @default.
- W4200311172 modified "2023-10-17" @default.
- W4200311172 title "Characterization and regulatory mechanism analysis of VvmiR156a– <i>VvAGL80</i> pair during grapevine flowering and parthenocarpy process induced by gibberellin" @default.
- W4200311172 cites W1980286688 @default.
- W4200311172 cites W1992956019 @default.
- W4200311172 cites W1998743995 @default.
- W4200311172 cites W2001836421 @default.
- W4200311172 cites W2003479420 @default.
- W4200311172 cites W2005993045 @default.
- W4200311172 cites W2007842740 @default.
- W4200311172 cites W2009539279 @default.
- W4200311172 cites W2011146068 @default.
- W4200311172 cites W2014832104 @default.
- W4200311172 cites W2027503834 @default.
- W4200311172 cites W2030989926 @default.
- W4200311172 cites W2032529493 @default.
- W4200311172 cites W2041122198 @default.
- W4200311172 cites W2047858731 @default.
- W4200311172 cites W2050460033 @default.
- W4200311172 cites W2052120683 @default.
- W4200311172 cites W2061499230 @default.
- W4200311172 cites W2063875041 @default.
- W4200311172 cites W2077440693 @default.
- W4200311172 cites W2078613795 @default.
- W4200311172 cites W2093095455 @default.
- W4200311172 cites W2098842350 @default.
- W4200311172 cites W2102924901 @default.
- W4200311172 cites W2119764005 @default.
- W4200311172 cites W2122575917 @default.
- W4200311172 cites W2127277786 @default.
- W4200311172 cites W2133761506 @default.
- W4200311172 cites W2134053059 @default.
- W4200311172 cites W2136383371 @default.
- W4200311172 cites W2138438425 @default.
- W4200311172 cites W2149199504 @default.
- W4200311172 cites W2150909263 @default.
- W4200311172 cites W2156257668 @default.
- W4200311172 cites W2162900827 @default.
- W4200311172 cites W2167681032 @default.
- W4200311172 cites W2172221673 @default.
- W4200311172 cites W2172245948 @default.
- W4200311172 cites W2333147647 @default.
- W4200311172 cites W2522637623 @default.
- W4200311172 cites W2592852964 @default.
- W4200311172 cites W2616375346 @default.
- W4200311172 cites W2735953210 @default.
- W4200311172 cites W2755900479 @default.
- W4200311172 cites W2770947706 @default.
- W4200311172 cites W2807067483 @default.
- W4200311172 cites W2807949675 @default.
- W4200311172 cites W2810976463 @default.
- W4200311172 cites W2940719422 @default.
- W4200311172 cites W3153307527 @default.
- W4200311172 doi "https://doi.org/10.1002/tpg2.20181" @default.
- W4200311172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34882981" @default.
- W4200311172 hasPublicationYear "2021" @default.
- W4200311172 type Work @default.
- W4200311172 citedByCount "2" @default.
- W4200311172 countsByYear W42003111722023 @default.
- W4200311172 crossrefType "journal-article" @default.
- W4200311172 hasAuthorship W4200311172A5013038112 @default.
- W4200311172 hasAuthorship W4200311172A5018688675 @default.
- W4200311172 hasAuthorship W4200311172A5026199531 @default.
- W4200311172 hasAuthorship W4200311172A5030246181 @default.
- W4200311172 hasAuthorship W4200311172A5035694858 @default.
- W4200311172 hasAuthorship W4200311172A5058171957 @default.
- W4200311172 hasAuthorship W4200311172A5064311978 @default.
- W4200311172 hasAuthorship W4200311172A5065495869 @default.
- W4200311172 hasAuthorship W4200311172A5072537482 @default.
- W4200311172 hasAuthorship W4200311172A5075086498 @default.
- W4200311172 hasAuthorship W4200311172A5085695020 @default.
- W4200311172 hasAuthorship W4200311172A5086529957 @default.
- W4200311172 hasBestOaLocation W42003111721 @default.
- W4200311172 hasConcept C104317684 @default.
- W4200311172 hasConcept C143065580 @default.
- W4200311172 hasConcept C156951646 @default.
- W4200311172 hasConcept C160956161 @default.
- W4200311172 hasConcept C187882448 @default.
- W4200311172 hasConcept C2779491563 @default.
- W4200311172 hasConcept C54355233 @default.
- W4200311172 hasConcept C59822182 @default.
- W4200311172 hasConcept C83740816 @default.
- W4200311172 hasConcept C86803240 @default.
- W4200311172 hasConceptScore W4200311172C104317684 @default.
- W4200311172 hasConceptScore W4200311172C143065580 @default.
- W4200311172 hasConceptScore W4200311172C156951646 @default.