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• W4200316174 endingPage "289" @default.
• W4200316174 startingPage "274" @default.
• W4200316174 abstract "The Mla (Mildew resistance locus a) of barley (Hordeum vulgare L.) is an effective model for cereal immunity against fungal pathogens. Like many resistance proteins, variants of the MLA coiled-coil nucleotide-binding leucine-rich repeat (CC-NLR) receptor often require the HRS complex (HSP90, RAR1, and SGT1) to function. However, functional analysis of Sgt1 has been particularly difficult, as deletions are often lethal. Recently, we identified rar3 (required for Mla6 resistance 3), an in-frame Sgt1ΔKL308-309 mutation in the SGT1-specific domain, that alters resistance conferred by MLA but without lethality. Here, we use autoactive MLA6 and recombinant yeast-two-hybrid strains with stably integrated HvRar1 and HvHsp90 to determine that this mutation weakens but does not entirely disrupt the interaction between SGT1 and MLA. This causes a concomitant reduction in MLA6 protein accumulation below the apparent threshold required for effective resistance. The ΔKL308-309 deletion had a lesser effect on intramolecular interactions than alanine or arginine substitutions, and MLA variants that display diminished interactions with SGT1 appear to be disproportionately affected by the SGT1ΔKL308-309 mutation. We hypothesize that those dimeric plant CC-NLRs that appear unaffected by Sgt1 silencing are those with the strongest intermolecular interactions with it. Combining our data with recent work in CC-NLRs, we propose a cyclical model of the MLA-HRS resistosome interactions.[Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 No Rights Reserved license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2022." @default.
• W4200316174 created "2021-12-31" @default.
• W4200316174 creator A5013925783 @default.
• W4200316174 creator A5026417439 @default.
• W4200316174 creator A5032426331 @default.
• W4200316174 creator A5055769588 @default.
• W4200316174 creator A5068125276 @default.
• W4200316174 date "2022-03-01" @default.
• W4200316174 modified "2023-10-18" @default.
• W4200316174 title "SGT1-Specific Domain Mutations Impair Interactions with the Barley MLA6 Immune Receptor in Association with Loss of NLR Protein" @default.
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• W4200316174 doi "https://doi.org/10.1094/mpmi-08-21-0217-r" @default.
• W4200316174 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34889653" @default.
• W4200316174 hasPublicationYear "2022" @default.

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