FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200327258> ?p ?o ?g. }

• W4200327258 endingPage "e12642" @default.
• W4200327258 startingPage "e12642" @default.
• W4200327258 abstract "Beak and feather disease virus (BFDV) is a circovirus that infects captive and wild psittacine birds, and is of conservation concern. The haemagglutination inhibition (HI) assay is used to determine antibody titres against BFDV, and the use of dried blood spots (DBS) on filter paper stored at room temperature has been suggested to be an equally valid technique to the use of frozen serum. However, research on other pathogens has found variable results when investigating the longevity of antibodies stored on DBS at room temperature. Consequently, we aimed to test the temporal stability of antibodies to BFDV in DBS samples stored long-term at room temperature. A further goal was to add to the current knowledge of antibody response to naturally acquired BFDV infection in crimson rosellas (Platycercus elegans).Blood was collected from wild P. elegans in Victoria, Australia, that had been live-trapped (n = 9) or necropsied (n = 11). BFDV virus load data were obtained from blood stored in ethanol by real-time quantitative PCR (qPCR); antibody titres were obtained by HI assay from either DBS or serum samples, which had been collected concurrently. All HI assays were performed commercially by the Veterinary Diagnostic Laboratory (VDL) in Charles Sturt University, Australia, who were blind to BFDV blood status.HI titres from DBS stored at room temperature declined significantly over time (~80 weeks). By contrast, frozen serum samples assayed after 80 weeks in storage all had high HI titres, only varying up to one dilution step from the initial HI titres obtained from DBS at 3-6 weeks after sampling. Weak HI titres from DBS samples all came back negative when the test was repeated only nine weeks later. Novel high HI titres were reported in P. elegans, and while most birds with high antibody titres had corresponding negative qPCR results, a single subadult presented with high HI titres and virus load simultaneously.Detection of antibodies on filter paper stored at room temperature decreases over time, increasing the chances of false negatives in these samples, and in repeated testing of samples with weak HI titres. Consequently, serum should be the preferred sample type to use for seroepidemiological studies on BFDV in parrots and other bird species. When not possible, it may help to store DBS on filter paper at -20 °C or lower. However, prompt testing of DBS samples (e.g., <6 weeks in storage) is recommended pending further research on antibody temporal stability. We also show that P. elegans, especially adults, can produce high antibody titres against BFDV, which may help them resist infection." @default.
• W4200327258 created "2021-12-31" @default.
• W4200327258 creator A5005691598 @default.
• W4200327258 creator A5049046480 @default.
• W4200327258 creator A5054154475 @default.
• W4200327258 creator A5069644923 @default.
• W4200327258 creator A5076161212 @default.
• W4200327258 date "2021-12-20" @default.
• W4200327258 modified "2023-09-23" @default.
• W4200327258 title "Ability to detect antibodies to beak and feather disease virus in blood on filter paper decreases with duration of storage" @default.
• W4200327258 cites W147206206 @default.
• W4200327258 cites W1857697770 @default.
• W4200327258 cites W1969413325 @default.
• W4200327258 cites W1969733959 @default.
• W4200327258 cites W1971389058 @default.
• W4200327258 cites W2000123580 @default.
• W4200327258 cites W2002863550 @default.
• W4200327258 cites W2002865740 @default.
• W4200327258 cites W2016590529 @default.
• W4200327258 cites W2028189497 @default.
• W4200327258 cites W2030865012 @default.
• W4200327258 cites W2037240245 @default.
• W4200327258 cites W2039754073 @default.
• W4200327258 cites W2044402365 @default.
• W4200327258 cites W2050727291 @default.
• W4200327258 cites W2060759286 @default.
• W4200327258 cites W2061114071 @default.
• W4200327258 cites W2130213398 @default.
• W4200327258 cites W2165558561 @default.
• W4200327258 cites W2169260123 @default.
• W4200327258 cites W2170810171 @default.
• W4200327258 cites W2250033442 @default.
• W4200327258 cites W2326376491 @default.
• W4200327258 cites W2399292674 @default.
• W4200327258 cites W2414694816 @default.
• W4200327258 cites W2764130197 @default.
• W4200327258 cites W2913773708 @default.
• W4200327258 cites W2965066857 @default.
• W4200327258 cites W2966571883 @default.
• W4200327258 cites W3034695756 @default.
• W4200327258 cites W3038144389 @default.
• W4200327258 cites W3038513683 @default.
• W4200327258 cites W3137778049 @default.
• W4200327258 cites W4290199722 @default.
• W4200327258 doi "https://doi.org/10.7717/peerj.12642" @default.
• W4200327258 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35036139" @default.
• W4200327258 hasPublicationYear "2021" @default.
• W4200327258 type Work @default.
• W4200327258 citedByCount "0" @default.
• W4200327258 crossrefType "journal-article" @default.
• W4200327258 hasAuthorship W4200327258A5005691598 @default.
• W4200327258 hasAuthorship W4200327258A5049046480 @default.
• W4200327258 hasAuthorship W4200327258A5054154475 @default.
• W4200327258 hasAuthorship W4200327258A5069644923 @default.
• W4200327258 hasAuthorship W4200327258A5076161212 @default.
• W4200327258 hasBestOaLocation W42003272581 @default.
• W4200327258 hasConcept C127745971 @default.
• W4200327258 hasConcept C142724271 @default.
• W4200327258 hasConcept C159047783 @default.
• W4200327258 hasConcept C159654299 @default.
• W4200327258 hasConcept C184112429 @default.
• W4200327258 hasConcept C185592680 @default.
• W4200327258 hasConcept C203014093 @default.
• W4200327258 hasConcept C204787440 @default.
• W4200327258 hasConcept C2522874641 @default.
• W4200327258 hasConcept C3017516299 @default.
• W4200327258 hasConcept C42972112 @default.
• W4200327258 hasConcept C43617362 @default.
• W4200327258 hasConcept C71924100 @default.
• W4200327258 hasConcept C86803240 @default.
• W4200327258 hasConcept C90856448 @default.
• W4200327258 hasConceptScore W4200327258C127745971 @default.
• W4200327258 hasConceptScore W4200327258C142724271 @default.
• W4200327258 hasConceptScore W4200327258C159047783 @default.
• W4200327258 hasConceptScore W4200327258C159654299 @default.
• W4200327258 hasConceptScore W4200327258C184112429 @default.
• W4200327258 hasConceptScore W4200327258C185592680 @default.
• W4200327258 hasConceptScore W4200327258C203014093 @default.
• W4200327258 hasConceptScore W4200327258C204787440 @default.
• W4200327258 hasConceptScore W4200327258C2522874641 @default.
• W4200327258 hasConceptScore W4200327258C3017516299 @default.
• W4200327258 hasConceptScore W4200327258C42972112 @default.
• W4200327258 hasConceptScore W4200327258C43617362 @default.
• W4200327258 hasConceptScore W4200327258C71924100 @default.
• W4200327258 hasConceptScore W4200327258C86803240 @default.
• W4200327258 hasConceptScore W4200327258C90856448 @default.
• W4200327258 hasFunder F4320334704 @default.
• W4200327258 hasLocation W42003272581 @default.
• W4200327258 hasLocation W42003272582 @default.
• W4200327258 hasLocation W42003272583 @default.
• W4200327258 hasLocation W42003272584 @default.
• W4200327258 hasOpenAccess W4200327258 @default.
• W4200327258 hasPrimaryLocation W42003272581 @default.
• W4200327258 hasRelatedWork W1487857142 @default.
• W4200327258 hasRelatedWork W1547625320 @default.
• W4200327258 hasRelatedWork W1561127041 @default.
• W4200327258 hasRelatedWork W2062279822 @default.
• W4200327258 hasRelatedWork W2099932062 @default.

• next