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- W4200397046 abstract "Summary Cleavage Under Targets & Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA Polymerase II (Pol2 Serine-5 phosphate) and an antibody to repressive Polycomb domains (H3K27me3) followed by computational signal deconvolution to produce high-resolution maps of both the active and repressive regulomes in single cells. The ability to seamlessly map active promoters, enhancers and repressive regulatory elements using a single workflow provides a complete regulome profiling strategy suitable for high-throughput single-cell platforms." @default.
- W4200397046 created "2021-12-31" @default.
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- W4200397046 date "2021-12-21" @default.
- W4200397046 modified "2023-10-03" @default.
- W4200397046 title "Simultaneous CUT&Tag profiling of the accessible and silenced regulome in single cells" @default.
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- W4200397046 doi "https://doi.org/10.1101/2021.12.19.473377" @default.
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