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• W4200399051 abstract "The production of (R)-α-phenylethylamine ((R)-α-PEA) from acetophenone is a classic reaction for the characterization of transaminases. However, developing a commercially viable transaminase process to manufacture (R)-α-PEA usually suffers from two drawbacks. One is related to the biocatalyst itself, since transaminases are easily inhibited by (R)-α-PEA at low concentrations. The other drawback is a common low space-time yield of typical transaminase processes, because the reaction equilibrium greatly favors the formation of acetophenone over (R)-α-PEA. In this study, an (R)-selective amine transaminase (TA) from Aspergillus fumigatus Af293 was engineered by a directed evolution for an efficient process to produce (R)-α-PEA. The evolved variant showed an over 3000-fold increase in activity and a tolerance with 2.0 M isopropylamine as well as the complete absence of inhibition by (R)-α-PEA. At the same time, using this evolved TA variant, a continuous neat organic process using whole-cells was developed where the biocatalyst and remaining acetophenone can be efficiently separated from (R)-α-PEA and reused repetitively. This not only decreases the overall cost and waste generation but also achieves a very high space-time yield of up to 168 g L–1 d–1 of (R)-α-PEA in an industrial pilot scale setup." @default.
• W4200399051 created "2021-12-31" @default.
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• W4200399051 date "2021-12-17" @default.
• W4200399051 modified "2023-10-06" @default.
• W4200399051 title "Transaminase Engineering and Process Development for a Whole-Cell Neat Organic Process to Produce (<i>R</i>)-α-Phenylethylamine" @default.
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• W4200399051 doi "https://doi.org/10.1021/acs.oprd.1c00409" @default.
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