Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200423353> ?p ?o ?g. }
- W4200423353 endingPage "1387" @default.
- W4200423353 startingPage "1387" @default.
- W4200423353 abstract "Peridinin-Chlorophyll a-Proteins (PCP) are the major light harvesting proteins in photosynthetic dinoflagellates. PCP shows great variation in protein length, pigment ratio, sequence, and spectroscopic properties. PCP conjugates (PerCP) are widely used as fluorescent probes for cellular and tissue analysis in the biomedical field. PCP consists of a peridinin carotenoid; thereby, it can potentially be used as a bioactive compound in pharmaceutical applications. However, the biological activities of PCP are yet to be explored. In this study, we extracted, purified, and partially characterised the PCP from Symbiodinium tridacnidorum (CS-73) and explored its antioxidant, anti-cancer and anti-inflammation bioactivities. The PCP was purified using an ÄKTA™ PURE system and predicted to be of 17.3 kDa molecular weight (confirmed as a single band on SDS-PAGE) with an isoelectric point (pI) 5.6. LC-MS/MS and bioinformatic analysis of purified PCP digested with trypsin indicated it was 164 amino acids long with >90% sequence similarity to PCP of SymA3.s6014_g3 (belonging to clade A of Symbiodinium sp.) confirmed with 59 peptide combinations matched across its protein sequence. The spectroscopic properties of purified PCP showed a slight shift in absorption and emission spectra to previously documented analysis in Symbiodinium species possibly due to variation in amino acid sequences that interact with chl a and peridinin. Purified PCP consisted of a 19-amino-acid-long signal peptide at its N terminal and nine helixes in its secondary structure, with several protein binding sites and no DNA/RNA binding site. Furthermore, purified PCP exhibited antioxidant and in vitro anti-inflammation bioactivities, and anti-cancer activities against human metastatic breast adenocarcinoma (MDA-MB-231) and human colorectal (HTC-15) cancer cell lines. Together, all these findings present PCP as a promising candidate for continued investigations for pharmaceutical applications to cure chronic diseases, apart from its existing application as a fluorescent-probe." @default.
- W4200423353 created "2021-12-31" @default.
- W4200423353 creator A5005034764 @default.
- W4200423353 creator A5016706027 @default.
- W4200423353 creator A5027568735 @default.
- W4200423353 creator A5030916597 @default.
- W4200423353 creator A5031307025 @default.
- W4200423353 creator A5050728421 @default.
- W4200423353 creator A5051149520 @default.
- W4200423353 creator A5053653555 @default.
- W4200423353 date "2021-12-06" @default.
- W4200423353 modified "2023-10-06" @default.
- W4200423353 title "Characterisation and Bioactivity Analysis of Peridinin-Chlorophyll a-Protein (PCP) Isolated from Symbiodinium tridacnidorum CS-73" @default.
- W4200423353 cites W1819502878 @default.
- W4200423353 cites W1965103740 @default.
- W4200423353 cites W1967867527 @default.
- W4200423353 cites W1982590711 @default.
- W4200423353 cites W2002200219 @default.
- W4200423353 cites W2004761977 @default.
- W4200423353 cites W2005368460 @default.
- W4200423353 cites W2007744435 @default.
- W4200423353 cites W2011598158 @default.
- W4200423353 cites W2012991711 @default.
- W4200423353 cites W2015490964 @default.
- W4200423353 cites W2033369384 @default.
- W4200423353 cites W2035816489 @default.
- W4200423353 cites W2037021175 @default.
- W4200423353 cites W2037665090 @default.
- W4200423353 cites W2038104480 @default.
- W4200423353 cites W2042817066 @default.
- W4200423353 cites W2044034583 @default.
- W4200423353 cites W2063987723 @default.
- W4200423353 cites W2066171146 @default.
- W4200423353 cites W2066859752 @default.
- W4200423353 cites W2068504832 @default.
- W4200423353 cites W2088259092 @default.
- W4200423353 cites W2089386118 @default.
- W4200423353 cites W2094458603 @default.
- W4200423353 cites W2106126535 @default.
- W4200423353 cites W2107707009 @default.
- W4200423353 cites W2109439026 @default.
- W4200423353 cites W2109884288 @default.
- W4200423353 cites W2118345984 @default.
- W4200423353 cites W2118810921 @default.
- W4200423353 cites W2119457180 @default.
- W4200423353 cites W2119757130 @default.
- W4200423353 cites W2127003168 @default.
- W4200423353 cites W2134668199 @default.
- W4200423353 cites W2136382837 @default.
- W4200423353 cites W2137826335 @default.
- W4200423353 cites W2154501851 @default.
- W4200423353 cites W2163751138 @default.
- W4200423353 cites W2167127749 @default.
- W4200423353 cites W2280346092 @default.
- W4200423353 cites W2493799904 @default.
- W4200423353 cites W2500005321 @default.
- W4200423353 cites W2611792444 @default.
- W4200423353 cites W2759477252 @default.
- W4200423353 cites W2788929286 @default.
- W4200423353 cites W2807342767 @default.
- W4200423353 cites W2808216887 @default.
- W4200423353 cites W2896610842 @default.
- W4200423353 cites W2911389002 @default.
- W4200423353 cites W2943551407 @default.
- W4200423353 cites W2963772184 @default.
- W4200423353 cites W3034813253 @default.
- W4200423353 cites W3040637243 @default.
- W4200423353 cites W3092526147 @default.
- W4200423353 cites W3121057999 @default.
- W4200423353 cites W3168034583 @default.
- W4200423353 doi "https://doi.org/10.3390/jmse9121387" @default.
- W4200423353 hasPublicationYear "2021" @default.
- W4200423353 type Work @default.
- W4200423353 citedByCount "6" @default.
- W4200423353 countsByYear W42004233532022 @default.
- W4200423353 countsByYear W42004233532023 @default.
- W4200423353 crossrefType "journal-article" @default.
- W4200423353 hasAuthorship W4200423353A5005034764 @default.
- W4200423353 hasAuthorship W4200423353A5016706027 @default.
- W4200423353 hasAuthorship W4200423353A5027568735 @default.
- W4200423353 hasAuthorship W4200423353A5030916597 @default.
- W4200423353 hasAuthorship W4200423353A5031307025 @default.
- W4200423353 hasAuthorship W4200423353A5050728421 @default.
- W4200423353 hasAuthorship W4200423353A5051149520 @default.
- W4200423353 hasAuthorship W4200423353A5053653555 @default.
- W4200423353 hasBestOaLocation W42004233531 @default.
- W4200423353 hasConcept C104317684 @default.
- W4200423353 hasConcept C167625842 @default.
- W4200423353 hasConcept C181199279 @default.
- W4200423353 hasConcept C2776408758 @default.
- W4200423353 hasConcept C2779281246 @default.
- W4200423353 hasConcept C2780340462 @default.
- W4200423353 hasConcept C2781200551 @default.
- W4200423353 hasConcept C2781231385 @default.
- W4200423353 hasConcept C28781525 @default.
- W4200423353 hasConcept C43144210 @default.
- W4200423353 hasConcept C515207424 @default.
- W4200423353 hasConcept C523546767 @default.