Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200427854> ?p ?o ?g. }
- W4200427854 endingPage "100126" @default.
- W4200427854 startingPage "100126" @default.
- W4200427854 abstract "Nucleic acid purification is a critical aspect of biomedical research and a multibillion-dollar industry. Here we establish sequence-selective RNA capture, release, and isolation using conformationally responsive DNA nanoswitches. We validate purification of specific RNAs ranging in size from 22 to 401 nt with up to 75% recovery and 99.98% purity in a benchtop process with minimal expense and equipment. Our method compared favorably with bead-based extraction of an endogenous microRNA from cellular total RNA, and can be programmed for multiplexed purification of multiple individual RNA targets from one sample. Coupling our approach with downstream LC/MS, we analyzed RNA modifications in 5.8S ribosomal RNA, and found 2′-O-methylguanosine, 2′-O-methyluridine, and pseudouridine in a ratio of ∼1:7:22. The simplicity, low cost, and low sample requirements of our method make it suitable for easy adoption, and the versatility of the approach provides opportunities to expand the strategy to other biomolecules." @default.
- W4200427854 created "2021-12-31" @default.
- W4200427854 creator A5003569237 @default.
- W4200427854 creator A5006114608 @default.
- W4200427854 creator A5008102971 @default.
- W4200427854 creator A5026888998 @default.
- W4200427854 creator A5037674579 @default.
- W4200427854 creator A5038030210 @default.
- W4200427854 creator A5040584832 @default.
- W4200427854 creator A5049808782 @default.
- W4200427854 creator A5049844296 @default.
- W4200427854 creator A5063928870 @default.
- W4200427854 creator A5064805798 @default.
- W4200427854 date "2021-12-01" @default.
- W4200427854 modified "2023-10-14" @default.
- W4200427854 title "Sequence-selective purification of biological RNAs using DNA nanoswitches" @default.
- W4200427854 cites W1492857902 @default.
- W4200427854 cites W1967317528 @default.
- W4200427854 cites W1971181518 @default.
- W4200427854 cites W1988941884 @default.
- W4200427854 cites W1991807334 @default.
- W4200427854 cites W1992598298 @default.
- W4200427854 cites W1996313751 @default.
- W4200427854 cites W2005877554 @default.
- W4200427854 cites W2011107379 @default.
- W4200427854 cites W2019403558 @default.
- W4200427854 cites W2042363053 @default.
- W4200427854 cites W2049055414 @default.
- W4200427854 cites W2061240424 @default.
- W4200427854 cites W2070777085 @default.
- W4200427854 cites W2082856142 @default.
- W4200427854 cites W2083537672 @default.
- W4200427854 cites W2126961173 @default.
- W4200427854 cites W2139316730 @default.
- W4200427854 cites W2144610319 @default.
- W4200427854 cites W2149017671 @default.
- W4200427854 cites W2161960347 @default.
- W4200427854 cites W2176803664 @default.
- W4200427854 cites W2189360969 @default.
- W4200427854 cites W2281682032 @default.
- W4200427854 cites W2312331183 @default.
- W4200427854 cites W2416700317 @default.
- W4200427854 cites W2557904026 @default.
- W4200427854 cites W2566723880 @default.
- W4200427854 cites W2611629280 @default.
- W4200427854 cites W2627064270 @default.
- W4200427854 cites W2769453731 @default.
- W4200427854 cites W2771458369 @default.
- W4200427854 cites W2789243590 @default.
- W4200427854 cites W2891167139 @default.
- W4200427854 cites W2897379742 @default.
- W4200427854 cites W2904330302 @default.
- W4200427854 cites W2915489297 @default.
- W4200427854 cites W2922214118 @default.
- W4200427854 cites W2948979661 @default.
- W4200427854 cites W2950280376 @default.
- W4200427854 cites W2951657988 @default.
- W4200427854 cites W2979086681 @default.
- W4200427854 cites W3006306973 @default.
- W4200427854 cites W3021255846 @default.
- W4200427854 cites W3080536083 @default.
- W4200427854 cites W3087172845 @default.
- W4200427854 cites W3118319793 @default.
- W4200427854 cites W4256485542 @default.
- W4200427854 doi "https://doi.org/10.1016/j.crmeth.2021.100126" @default.
- W4200427854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35072148" @default.
- W4200427854 hasPublicationYear "2021" @default.
- W4200427854 type Work @default.
- W4200427854 citedByCount "4" @default.
- W4200427854 countsByYear W42004278542022 @default.
- W4200427854 countsByYear W42004278542023 @default.
- W4200427854 crossrefType "journal-article" @default.
- W4200427854 hasAuthorship W4200427854A5003569237 @default.
- W4200427854 hasAuthorship W4200427854A5006114608 @default.
- W4200427854 hasAuthorship W4200427854A5008102971 @default.
- W4200427854 hasAuthorship W4200427854A5026888998 @default.
- W4200427854 hasAuthorship W4200427854A5037674579 @default.
- W4200427854 hasAuthorship W4200427854A5038030210 @default.
- W4200427854 hasAuthorship W4200427854A5040584832 @default.
- W4200427854 hasAuthorship W4200427854A5049808782 @default.
- W4200427854 hasAuthorship W4200427854A5049844296 @default.
- W4200427854 hasAuthorship W4200427854A5063928870 @default.
- W4200427854 hasAuthorship W4200427854A5064805798 @default.
- W4200427854 hasBestOaLocation W42004278541 @default.
- W4200427854 hasConcept C104317684 @default.
- W4200427854 hasConcept C112190416 @default.
- W4200427854 hasConcept C185592680 @default.
- W4200427854 hasConcept C21951064 @default.
- W4200427854 hasConcept C24107716 @default.
- W4200427854 hasConcept C43617362 @default.
- W4200427854 hasConcept C49853544 @default.
- W4200427854 hasConcept C552990157 @default.
- W4200427854 hasConcept C55493867 @default.
- W4200427854 hasConcept C67705224 @default.
- W4200427854 hasConcept C70721500 @default.
- W4200427854 hasConcept C86803240 @default.
- W4200427854 hasConceptScore W4200427854C104317684 @default.
- W4200427854 hasConceptScore W4200427854C112190416 @default.