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- W4200433257 abstract "Abstract Background Hair follicle (HF) undergo periodic growth and development in mammals, which regulated by dermal papilla cells (DPCs) are reported to play an important role in the HF morphogenesis and development. However, primary DPCs have low proliferative activity, age quickly, and fresh cell isolation is both time-consuming and laborious. Method In this study, we introduced SV40LT into dissociated early passage rabbit vibrissae DPCs with lentiviral vectors and established seven immortalized DP cell lines (R-1, R-2, R-3, R-4, R-5, R-6 and R-7). Result These cell lines displayed early passage morphology and displayed high alkaline phosphatase activity. RT-PCR and immunofluorescence staining showed that all the immortalized cell lines expressed the DPC markers ( α-SMA ,IGF1 , ALPL , FGF2 , BMP2 and TGFβ2 ; α-SMA and VIM protein), but α-SMA was only expressed well in R-3, R-4, and R-7. Furthermore, it was found that R-7 was the only line to survive beyond 50 passages. Compared to melanoma cells, R-7 did not undergo malignant transformation. Karyotyping and cell growth viability analysis illustrated that the R-7 cell line preserved the basic characteristics of primary DPCs. Conclusion The R-7 DPCs established have potential application for future hair research. The study provides the theoretical basis in the cell research of HF growth and development." @default.
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- W4200433257 date "2021-12-07" @default.
- W4200433257 modified "2023-10-07" @default.
- W4200433257 title "Establishment and Functional Characterization of Immortalized Rabbit Dermal Papilla Cell Lines" @default.
- W4200433257 doi "https://doi.org/10.21203/rs.3.rs-1105281/v1" @default.
- W4200433257 hasPublicationYear "2021" @default.
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