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- W4200445802 abstract "Entamoeba histolytica is the causative agent of amoebic dysentery and liver abscess in humans. The parasitic lifestyle and the virulence of the protist requires elaborate biological processes, including vesicular traffic and stress management against a variety of reactive oxygen and nitrogen species produced by host immune response. Although the mechanisms for intracellular traffic of representative virulence factors have been investigated at the molecular levels, it remains poorly understood whether and how intracellular traffic is involved in the defense against reactive oxygen and nitrogen species. Here, we demonstrate that EhArfX2, one of the Arf family of GTPases known to be involved in the regulation of vesicular traffic, was identified by comparative transcriptomic analysis of two isogenic strains: an animal-passaged highly virulent HM-1:IMSS Cl6 and in vitro maintained attenuated avirulent strain. ArfA2 was identified as one of the most highly upregulated genes in the highly virulent strain. EhArfX2 was localized to small vesicle-like structures, and largely colocalized with the markers for the trans-Golgi network SNARE, Ykt6, but neither with the ER-resident chaperon, Bip, nor the cis-Golgi SNARE, EhSed5, and Golgi-luminal galactosyl transferase, EhGalT. Expression of the dominant-active mutant form of EhArfX2 caused an increase in the number of lysosomes, while expression of the dominant-negative mutant led to defect in lysosome formation and cysteine protease transport to lysosomes. Expression of the dominant-negative mutant in the virulent E. histolytica strain caused reduction of the size of liver abscesses in a hamster model. This defect in liver abscess formation was likely at least partially attributed to reduce resistance to nitrosative, but not oxidative stress in vitro. These results showed that the ArfX2-mediated traffic is necessary for the nitrosative stress response and virulence in the host." @default.
- W4200445802 created "2021-12-31" @default.
- W4200445802 date "2021-12-17" @default.
- W4200445802 modified "2023-09-29" @default.
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- W4200445802 doi "https://doi.org/10.3389/fcimb.2021.794152.s007" @default.
- W4200445802 hasPublicationYear "2021" @default.
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