Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200458532> ?p ?o ?g. }
- W4200458532 endingPage "114368" @default.
- W4200458532 startingPage "114368" @default.
- W4200458532 abstract "The hormone autotrophic cell (HAC) line of Aralia elata grows quickly in vitro without a plant-growth regulator and is thus a potential platform cell line for high-value metabolite production. In this study, a suspension culture of this HAC line was established by optimizing the concentration of sucrose (30–90 g/L), the inoculation amount, and the strength of the Murashige and Skoog (MS) medium. Then, different concentrations of the elicitors methyl jasmonate (MJ; 0, 50, 100, 250, and 500 μmol/L) and salicylic acid (SA; 0, 10, 25, 50, 100 and 250 mg/L) were used to improve its oleanolic acid accumulation, and the precursors phenylalanine, sodium acetate, and cinnamic acid were screened to enhance flavonoid accumulation. The optimal conditions for HAC line growth were 1.0 g HACs suspended in 100 mL liquid MS medium (in a 250-mL flask) with the addition of 70 g/L sucrose, and the time-points for the highest accumulations of oleanolic acid and total flavonoids were 18 and 21 days, respectively. The treatment of HACs with 250 μmol/L MJ for 6 days or 25 mg/L SA for 3 days improved oleanolic acid accumulation efficiency by 1.597-times (12.065 μg/d) and 3.59-times (22.860 μg/d) that of the control, respectively. The most effective concentrations of the three precursors were 50 mg/L for phenylalanine and sodium acetate, and 5 mg/L for cinnamic acid. Of these, sodium acetate treatment for 14 days proved most effective, providing a total flavonoids content (4.124 mg/L) 1.87-times that of the control and an accumulation efficiency 2.37-times that of the control. Therefore, the most effective treatments for HACs after 10 days of culturing were 25 mg/L SA for 3 days and 5 mg/L sodium acetate for 14 days, which improve the accumulations of oleanolic acid and total flavonoids, respectively." @default.
- W4200458532 created "2021-12-31" @default.
- W4200458532 creator A5016723814 @default.
- W4200458532 creator A5040529742 @default.
- W4200458532 creator A5051201595 @default.
- W4200458532 creator A5057737324 @default.
- W4200458532 creator A5067321240 @default.
- W4200458532 creator A5075679556 @default.
- W4200458532 date "2022-02-01" @default.
- W4200458532 modified "2023-09-27" @default.
- W4200458532 title "A suspension culture of the hormone autotrophic cell line of Aralia elata (Miq.) Seem. for production of oleanolic acid and flavonoids" @default.
- W4200458532 cites W1843080077 @default.
- W4200458532 cites W1964656084 @default.
- W4200458532 cites W1978045066 @default.
- W4200458532 cites W1979238416 @default.
- W4200458532 cites W1979879526 @default.
- W4200458532 cites W1984291507 @default.
- W4200458532 cites W2014444134 @default.
- W4200458532 cites W2024556021 @default.
- W4200458532 cites W2025404024 @default.
- W4200458532 cites W2037786098 @default.
- W4200458532 cites W2049698074 @default.
- W4200458532 cites W2052019842 @default.
- W4200458532 cites W2053475743 @default.
- W4200458532 cites W2079734238 @default.
- W4200458532 cites W2089150025 @default.
- W4200458532 cites W2091794990 @default.
- W4200458532 cites W2094477618 @default.
- W4200458532 cites W2097043803 @default.
- W4200458532 cites W2111359577 @default.
- W4200458532 cites W2170551352 @default.
- W4200458532 cites W2178297348 @default.
- W4200458532 cites W2305703339 @default.
- W4200458532 cites W2508036849 @default.
- W4200458532 cites W2516476685 @default.
- W4200458532 cites W2569756946 @default.
- W4200458532 cites W2802447663 @default.
- W4200458532 cites W2883474297 @default.
- W4200458532 cites W2886031409 @default.
- W4200458532 cites W2887686774 @default.
- W4200458532 cites W2965976033 @default.
- W4200458532 cites W3015135689 @default.
- W4200458532 cites W3025546635 @default.
- W4200458532 cites W3028455568 @default.
- W4200458532 cites W3088801833 @default.
- W4200458532 cites W3107514733 @default.
- W4200458532 cites W3118275827 @default.
- W4200458532 cites W3182115552 @default.
- W4200458532 doi "https://doi.org/10.1016/j.indcrop.2021.114368" @default.
- W4200458532 hasPublicationYear "2022" @default.
- W4200458532 type Work @default.
- W4200458532 citedByCount "1" @default.
- W4200458532 countsByYear W42004585322023 @default.
- W4200458532 crossrefType "journal-article" @default.
- W4200458532 hasAuthorship W4200458532A5016723814 @default.
- W4200458532 hasAuthorship W4200458532A5040529742 @default.
- W4200458532 hasAuthorship W4200458532A5051201595 @default.
- W4200458532 hasAuthorship W4200458532A5057737324 @default.
- W4200458532 hasAuthorship W4200458532A5067321240 @default.
- W4200458532 hasAuthorship W4200458532A5075679556 @default.
- W4200458532 hasConcept C104317684 @default.
- W4200458532 hasConcept C142724271 @default.
- W4200458532 hasConcept C157890192 @default.
- W4200458532 hasConcept C185592680 @default.
- W4200458532 hasConcept C200806820 @default.
- W4200458532 hasConcept C202751555 @default.
- W4200458532 hasConcept C204787440 @default.
- W4200458532 hasConcept C2776673659 @default.
- W4200458532 hasConcept C2777130120 @default.
- W4200458532 hasConcept C2777431362 @default.
- W4200458532 hasConcept C2777477808 @default.
- W4200458532 hasConcept C2777942875 @default.
- W4200458532 hasConcept C2778221352 @default.
- W4200458532 hasConcept C2778597767 @default.
- W4200458532 hasConcept C2778831176 @default.
- W4200458532 hasConcept C2778858181 @default.
- W4200458532 hasConcept C31903555 @default.
- W4200458532 hasConcept C43617362 @default.
- W4200458532 hasConcept C515207424 @default.
- W4200458532 hasConcept C55493867 @default.
- W4200458532 hasConcept C59822182 @default.
- W4200458532 hasConcept C71924100 @default.
- W4200458532 hasConcept C86803240 @default.
- W4200458532 hasConceptScore W4200458532C104317684 @default.
- W4200458532 hasConceptScore W4200458532C142724271 @default.
- W4200458532 hasConceptScore W4200458532C157890192 @default.
- W4200458532 hasConceptScore W4200458532C185592680 @default.
- W4200458532 hasConceptScore W4200458532C200806820 @default.
- W4200458532 hasConceptScore W4200458532C202751555 @default.
- W4200458532 hasConceptScore W4200458532C204787440 @default.
- W4200458532 hasConceptScore W4200458532C2776673659 @default.
- W4200458532 hasConceptScore W4200458532C2777130120 @default.
- W4200458532 hasConceptScore W4200458532C2777431362 @default.
- W4200458532 hasConceptScore W4200458532C2777477808 @default.
- W4200458532 hasConceptScore W4200458532C2777942875 @default.
- W4200458532 hasConceptScore W4200458532C2778221352 @default.
- W4200458532 hasConceptScore W4200458532C2778597767 @default.
- W4200458532 hasConceptScore W4200458532C2778831176 @default.