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• W4200464768 abstract "Abstract One of the main challenges in modern tissue engineering is to design biocompatible scaffolds with finely tuned porous architecture and capacity to load bioactive molecules that guide the growth and differentiation of the cells during tissue reconstruction. This work proposes a strategy to design porous alginate scaffolds (PAS) with well‐tuned architecture by leaching of sacrificial vaterite CaCO 3 microspheres packed in alginate. Pore size and interconnectivity depend on CaCO 3 sphere dimensions and packing as well as alginate concentration. Varying of these parameters, almost hundred percent pore interconnectivity (or, by contrast, a zero pore interconnectivity) can be achieved. Junctions between interconnected pores are about 50–70% of the pore dimensions that provides molecular transport through the PASs potentially ensuring diffusion of nutrition, oxygen and metabolic products when cell seeding. An opportunity to fabricate a multifunctional scaffold is demonstrated by encapsulation of desired macromolecules into the individual pores of a scaffold (is illustrated by dextran loading). Mechanical properties of PASs are found typical for soft and hydrated structures (Young's modulus of 19 ± 15 kPa) which is appropriate for cell seeding. The three cell lines (HeLa, HEK293, and L929) are cultured on different alginate scaffolds to examine cell viability and adhesiveness." @default.
• W4200464768 created "2021-12-31" @default.
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• W4200464768 date "2021-12-18" @default.
• W4200464768 modified "2023-10-16" @default.
• W4200464768 title "Porous Alginate Scaffolds Designed by Calcium Carbonate Leaching Technique" @default.
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• W4200464768 doi "https://doi.org/10.1002/adfm.202109824" @default.
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