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• W4200475519 abstract "GSH, Cys, Hcy, and H2S are important biothiols and play important roles in the living systems. Quantitative and simultaneous determination of these biothiols under physiological conditions is still a challenge. Herein, we developed an effective 19F-reactive tag that readily interacts with these four biothiols for the generation of stable thioether products that have distinguishable 19F-chemical shifts. These thioester compounds encode the characteristic fingerprint profiles of each biothiols, allowing one to simultaneously quantify and determine these biothiols by 1D 19F NMR spectroscopy. The intra-/extracellular GSH in live cells was assessed by the established strategy, and remarkable variations in the GSH stability were determined between the normal mammalian cells and cancer cells. It is notable that GSH hydrolyzes efficiently in the out-membrane of the cancer cells and the lysates. In contrast, GSH remains stable in the tested normal cells." @default.
• W4200475519 created "2021-12-31" @default.
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• W4200475519 date "2021-12-27" @default.
• W4200475519 modified "2023-10-14" @default.
• W4200475519 title "Simultaneous Quantification of Biothiols and Deciphering Diverse GSH Stability in Different Live Cells by ¹⁹F-Tag" @default.
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• W4200475519 doi "https://doi.org/10.1021/acs.analchem.1c03673" @default.
• W4200475519 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34958555" @default.
• W4200475519 hasPublicationYear "2021" @default.
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