FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200483721> ?p ?o ?g. }

• W4200483721 endingPage "55" @default.
• W4200483721 startingPage "49" @default.
• W4200483721 abstract "The amniotic fluid contains a large population of stem keratinocytes demonstrating minimal immunological rejection. Recent evidence suggests that stem cells from the amniotic fluid can be employed in the field of tissue engineering. In this work we identified precursors of the epithelial cells and expanded them in vitro.After collecting samples of amniotic fluid and separating the cells via centrifugation, we seeded a portion of these cells in selection media to analyze the proliferation of epithelial cells. The stem cells precursors of keratinocytes were identified through specific markers. The expression of these markers was evaluated by immunofluorescence and reverse transcription polymerase chain reaction (PCR).The stem cells demonstrated 90% confluence, after undergoing proliferation in the selection medium for 15 days. Most of these cells tested positive for the keratinocyte-specific markers, but negative for stem cell specific markers. Of note, the identity of the keratinocytes was well established even after several subcultures.These results suggested that it is feasible to isolate and expand differentiated cell populations in the amniotic fluid from precursor cells. Furthermore, amniotic membranes can be utilized as scaffolds to grow keratinocytes, which can be potentially exploited in areas of skin ulcer transplantation and tissue engineering interventions." @default.
• W4200483721 created "2021-12-31" @default.
• W4200483721 creator A5002842523 @default.
• W4200483721 creator A5003317806 @default.
• W4200483721 creator A5006269103 @default.
• W4200483721 creator A5015449528 @default.
• W4200483721 creator A5023347140 @default.
• W4200483721 creator A5031820628 @default.
• W4200483721 creator A5039703810 @default.
• W4200483721 creator A5045120276 @default.
• W4200483721 creator A5047979524 @default.
• W4200483721 creator A5054668928 @default.
• W4200483721 creator A5058964694 @default.
• W4200483721 creator A5063436761 @default.
• W4200483721 creator A5077273016 @default.
• W4200483721 creator A5078636303 @default.
• W4200483721 creator A5084568089 @default.
• W4200483721 date "2021-12-01" @default.
• W4200483721 modified "2023-10-17" @default.
• W4200483721 title "In vitro cell culture of amniotic fluid keratinocytes on amniotic membrane: the ideal tissue for repairing skin ulcers." @default.
• W4200483721 doi "https://doi.org/10.26355/eurrev_202112_27333" @default.
• W4200483721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34890034" @default.
• W4200483721 hasPublicationYear "2021" @default.
• W4200483721 type Work @default.
• W4200483721 citedByCount "0" @default.
• W4200483721 crossrefType "journal-article" @default.
• W4200483721 hasAuthorship W4200483721A5002842523 @default.
• W4200483721 hasAuthorship W4200483721A5003317806 @default.
• W4200483721 hasAuthorship W4200483721A5006269103 @default.
• W4200483721 hasAuthorship W4200483721A5015449528 @default.
• W4200483721 hasAuthorship W4200483721A5023347140 @default.
• W4200483721 hasAuthorship W4200483721A5031820628 @default.
• W4200483721 hasAuthorship W4200483721A5039703810 @default.
• W4200483721 hasAuthorship W4200483721A5045120276 @default.
• W4200483721 hasAuthorship W4200483721A5047979524 @default.
• W4200483721 hasAuthorship W4200483721A5054668928 @default.
• W4200483721 hasAuthorship W4200483721A5058964694 @default.
• W4200483721 hasAuthorship W4200483721A5063436761 @default.
• W4200483721 hasAuthorship W4200483721A5077273016 @default.
• W4200483721 hasAuthorship W4200483721A5078636303 @default.
• W4200483721 hasAuthorship W4200483721A5084568089 @default.
• W4200483721 hasConcept C109661977 @default.
• W4200483721 hasConcept C123012128 @default.
• W4200483721 hasConcept C138851942 @default.
• W4200483721 hasConcept C141071460 @default.
• W4200483721 hasConcept C172680121 @default.
• W4200483721 hasConcept C185592680 @default.
• W4200483721 hasConcept C202751555 @default.
• W4200483721 hasConcept C203014093 @default.
• W4200483721 hasConcept C20875687 @default.
• W4200483721 hasConcept C2777074287 @default.
• W4200483721 hasConcept C2778194526 @default.
• W4200483721 hasConcept C2779234561 @default.
• W4200483721 hasConcept C28328180 @default.
• W4200483721 hasConcept C2908647359 @default.
• W4200483721 hasConcept C2911091166 @default.
• W4200483721 hasConcept C54355233 @default.
• W4200483721 hasConcept C55493867 @default.
• W4200483721 hasConcept C71924100 @default.
• W4200483721 hasConcept C86803240 @default.
• W4200483721 hasConcept C95444343 @default.
• W4200483721 hasConcept C99454951 @default.
• W4200483721 hasConceptScore W4200483721C109661977 @default.
• W4200483721 hasConceptScore W4200483721C123012128 @default.
• W4200483721 hasConceptScore W4200483721C138851942 @default.
• W4200483721 hasConceptScore W4200483721C141071460 @default.
• W4200483721 hasConceptScore W4200483721C172680121 @default.
• W4200483721 hasConceptScore W4200483721C185592680 @default.
• W4200483721 hasConceptScore W4200483721C202751555 @default.
• W4200483721 hasConceptScore W4200483721C203014093 @default.
• W4200483721 hasConceptScore W4200483721C20875687 @default.
• W4200483721 hasConceptScore W4200483721C2777074287 @default.
• W4200483721 hasConceptScore W4200483721C2778194526 @default.
• W4200483721 hasConceptScore W4200483721C2779234561 @default.
• W4200483721 hasConceptScore W4200483721C28328180 @default.
• W4200483721 hasConceptScore W4200483721C2908647359 @default.
• W4200483721 hasConceptScore W4200483721C2911091166 @default.
• W4200483721 hasConceptScore W4200483721C54355233 @default.
• W4200483721 hasConceptScore W4200483721C55493867 @default.
• W4200483721 hasConceptScore W4200483721C71924100 @default.
• W4200483721 hasConceptScore W4200483721C86803240 @default.
• W4200483721 hasConceptScore W4200483721C95444343 @default.
• W4200483721 hasConceptScore W4200483721C99454951 @default.
• W4200483721 hasIssue "1 Suppl" @default.
• W4200483721 hasLocation W42004837211 @default.
• W4200483721 hasOpenAccess W4200483721 @default.
• W4200483721 hasPrimaryLocation W42004837211 @default.
• W4200483721 hasRelatedWork W10550394 @default.
• W4200483721 hasRelatedWork W12034155 @default.
• W4200483721 hasRelatedWork W2970846 @default.
• W4200483721 hasRelatedWork W301943 @default.
• W4200483721 hasRelatedWork W6369080 @default.
• W4200483721 hasRelatedWork W6614145 @default.
• W4200483721 hasRelatedWork W6668492 @default.
• W4200483721 hasRelatedWork W6853142 @default.
• W4200483721 hasRelatedWork W7441089 @default.
• W4200483721 hasRelatedWork W407774 @default.
• W4200483721 hasVolume "25" @default.

• next