Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200486071> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W4200486071 endingPage "147" @default.
- W4200486071 startingPage "137" @default.
- W4200486071 abstract "Although it has been reported that the anti-diabetic drug metformin has multiple extra-hypoglycemic activities, such as anti-oxidation, anti-aging and even anti-tumor, topical metformin also can induce hair regeneration, but the precise mechanism involved in that process is still unclear.To assess the effect of metformin on hair growth in a mouse hair follicle reconstitution model generated by in vitro self-assembled three-dimensional aggregates of epidermal and dermal cells (3D aggregates).Epidermal cells and dermal cells were isolated and cultured from the mouse skin of fifty C57BL/6 mouse pups (1-day-old). For tracing the distribution of dermal cells during the self-assembly process of 3D aggregates, the dermal cells were labeled with Vybrant Dil cell-labelling solution and mixed with epidermal cells at 1:1 ratio. Formed 3D aggregates were treated with 10 mM metformin and then were grafted into recipient BALB/c nude mice. The biomarkers (HGF, CD133, ALP, β-catenin and SOX2) associated with the hair-inductive activity of dermal cells were detected in the grafted skin tissues and in cultured 3D aggregates treated with metformin using immunofluorescent staining, quantitative real-time RT-PCR (qRT-PCR), and western blotting. Furthermore, the expression levels of CD133 were also examined in dermal cells with different passage numbers using qRT-PCR and western blotting.Metformin directly stimulates the activity of alkaline phosphatase (ALP) of cultured 3D aggregates, upregulates both the protein and mRNA expression levels of molecular markers (HGF, CD133, ALP, β-catenin and SOX2) and improves the survival rate of reconstituted hair follicles. Moreover, we also found that metformin increases the expression of CD133 in dermal cells thus maintaining their trichogenic capacity that would normally be lost by serial subculture.These results suggest that metformin can promote hair follicle regeneration in vitro through up-regulation of the hair inductive capability of dermal cells, warranting further evaluation in the clinical treatment of male or female pattern hair loss." @default.
- W4200486071 created "2021-12-31" @default.
- W4200486071 creator A5022687954 @default.
- W4200486071 creator A5029964155 @default.
- W4200486071 creator A5041838267 @default.
- W4200486071 creator A5047956894 @default.
- W4200486071 creator A5055315525 @default.
- W4200486071 creator A5081830070 @default.
- W4200486071 date "2021-12-09" @default.
- W4200486071 modified "2023-10-16" @default.
- W4200486071 title "Metformin Promotes the Hair-Inductive Activity of Three-Dimensional Aggregates of Epidermal and Dermal Cells Self-Assembled in vitro" @default.
- W4200486071 cites W1501867271 @default.
- W4200486071 cites W1631740935 @default.
- W4200486071 cites W1975847309 @default.
- W4200486071 cites W2139245193 @default.
- W4200486071 cites W2490742460 @default.
- W4200486071 cites W2539225005 @default.
- W4200486071 cites W2605523477 @default.
- W4200486071 cites W2751676921 @default.
- W4200486071 cites W2763358172 @default.
- W4200486071 cites W2783544481 @default.
- W4200486071 cites W2890302300 @default.
- W4200486071 cites W2968810751 @default.
- W4200486071 cites W3002878877 @default.
- W4200486071 cites W3016485033 @default.
- W4200486071 cites W3183145297 @default.
- W4200486071 doi "https://doi.org/10.1159/000521400" @default.
- W4200486071 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34883492" @default.
- W4200486071 hasPublicationYear "2021" @default.
- W4200486071 type Work @default.
- W4200486071 citedByCount "5" @default.
- W4200486071 countsByYear W42004860712023 @default.
- W4200486071 crossrefType "journal-article" @default.
- W4200486071 hasAuthorship W4200486071A5022687954 @default.
- W4200486071 hasAuthorship W4200486071A5029964155 @default.
- W4200486071 hasAuthorship W4200486071A5041838267 @default.
- W4200486071 hasAuthorship W4200486071A5047956894 @default.
- W4200486071 hasAuthorship W4200486071A5055315525 @default.
- W4200486071 hasAuthorship W4200486071A5081830070 @default.
- W4200486071 hasConcept C104317684 @default.
- W4200486071 hasConcept C134018914 @default.
- W4200486071 hasConcept C153911025 @default.
- W4200486071 hasConcept C160160445 @default.
- W4200486071 hasConcept C181199279 @default.
- W4200486071 hasConcept C185592680 @default.
- W4200486071 hasConcept C202751555 @default.
- W4200486071 hasConcept C2777074287 @default.
- W4200486071 hasConcept C2777216303 @default.
- W4200486071 hasConcept C2777933648 @default.
- W4200486071 hasConcept C2780323712 @default.
- W4200486071 hasConcept C2781085983 @default.
- W4200486071 hasConcept C55493867 @default.
- W4200486071 hasConcept C555293320 @default.
- W4200486071 hasConcept C85265743 @default.
- W4200486071 hasConcept C86339819 @default.
- W4200486071 hasConcept C86803240 @default.
- W4200486071 hasConcept C95444343 @default.
- W4200486071 hasConceptScore W4200486071C104317684 @default.
- W4200486071 hasConceptScore W4200486071C134018914 @default.
- W4200486071 hasConceptScore W4200486071C153911025 @default.
- W4200486071 hasConceptScore W4200486071C160160445 @default.
- W4200486071 hasConceptScore W4200486071C181199279 @default.
- W4200486071 hasConceptScore W4200486071C185592680 @default.
- W4200486071 hasConceptScore W4200486071C202751555 @default.
- W4200486071 hasConceptScore W4200486071C2777074287 @default.
- W4200486071 hasConceptScore W4200486071C2777216303 @default.
- W4200486071 hasConceptScore W4200486071C2777933648 @default.
- W4200486071 hasConceptScore W4200486071C2780323712 @default.
- W4200486071 hasConceptScore W4200486071C2781085983 @default.
- W4200486071 hasConceptScore W4200486071C55493867 @default.
- W4200486071 hasConceptScore W4200486071C555293320 @default.
- W4200486071 hasConceptScore W4200486071C85265743 @default.
- W4200486071 hasConceptScore W4200486071C86339819 @default.
- W4200486071 hasConceptScore W4200486071C86803240 @default.
- W4200486071 hasConceptScore W4200486071C95444343 @default.
- W4200486071 hasIssue "3" @default.
- W4200486071 hasLocation W42004860711 @default.
- W4200486071 hasLocation W42004860712 @default.
- W4200486071 hasOpenAccess W4200486071 @default.
- W4200486071 hasPrimaryLocation W42004860711 @default.
- W4200486071 hasRelatedWork W1997194464 @default.
- W4200486071 hasRelatedWork W2013584852 @default.
- W4200486071 hasRelatedWork W2015770429 @default.
- W4200486071 hasRelatedWork W2038128569 @default.
- W4200486071 hasRelatedWork W2039077395 @default.
- W4200486071 hasRelatedWork W2041819610 @default.
- W4200486071 hasRelatedWork W2073750598 @default.
- W4200486071 hasRelatedWork W2120165404 @default.
- W4200486071 hasRelatedWork W2138610498 @default.
- W4200486071 hasRelatedWork W2520362836 @default.
- W4200486071 hasVolume "35" @default.
- W4200486071 isParatext "false" @default.
- W4200486071 isRetracted "false" @default.
- W4200486071 workType "article" @default.