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- W4200499711 abstract "Abstract The use of propagation-invariant Airy beams enables a light-sheet microscopy with a large field-of-view. Without relying upon two-photon excitation or deconvolution-based processing to eliminate out-of focus blur caused by the side lobes, here, we present how the subtraction method is applied to enhance the image quality in digital scanned light-sheet microscopy with Airy beam. In the proposed method, planar Airy beam with the symmetric transversal structure is used to excite the sample. A hollow Airy beam with zero intensity at the focal plane is created, which is mainly used to excite the out-of-focus signal. By scanning the sample twice with the normal planar Airy beam and the hollow Airy beam, digital post-processing of the obtained images by subtraction allows for the rejection of out-of-focus blur and improves the optical sectioning, the axial resolution and the intensity distribution uniformity of the light-sheet microscopy." @default.
- W4200499711 created "2021-12-31" @default.
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- W4200499711 date "2022-01-04" @default.
- W4200499711 modified "2023-09-26" @default.
- W4200499711 title "Enhancement of image quality in planar Airy light-sheet microscopy via subtraction method" @default.
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- W4200499711 doi "https://doi.org/10.1088/2040-8986/ac4437" @default.
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