Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200506368> ?p ?o ?g. }
- W4200506368 abstract "Klebsiella oxytoca is an important nosocomial and community-acquired opportunistic pathogenic Klebsiella and has become the second most prevalent strain in the clinic after K. pneumoniae. However, there have been few reports of bacteriophages used for treating K. oxytoca. In this study, a novel bacteriophage, vB_Kox_ZX8, which specifically infects K. oxytoca AD3, was isolated for the first time from human fecal samples. The biological characteristics of vB_Kox_ZX8 showed an incubation period of 10 min, a burst size of 74 PFU/cell, and a stable pH range of 3-11. Genomic bioinformatics studies of vB_Kox_ZX8 showed that it belongs to the genus Przondovirus, subfamily Studiervirinae, family Autographiviridae. The genome of vB_Kox_ZX8 is 39,398 bp in length and contains 46 putative open reading frames encoding functional proteins, such as DNA degradation, packaging, structural, lysin-holin, and hypothetical proteins. We further investigated the efficacy of vB_Kox_ZX8 phage in the treatment of mice with bacteremia caused by K. oxytoca infection. The results showed that vB_Kox_ZX8 (5 × 109 PFU/mouse) injected intraperitoneally alone was metabolized rapidly in BALB/c mice, and no significant side effects were observed in the control and treatment groups. Importantly, intraperitoneal injection with a single dose of phage vB_Kox_ZX8 (5 × 107 PFU/mouse) for 1 h post-infection saved 100% of BALB/c mice from bacteremia induced by intraperitoneal challenge with a minimum lethal dose of K. oxytoca AD3. However, all negative control mice injected with PBS alone died. Owing to its good safety, narrow host infectivity, high lysis efficiency in vitro, and good in vivo therapeutic effect, phage vB_Kox_ZX8 has the potential to be an excellent antibacterial agent for clinical K. oxytoca-caused infections." @default.
- W4200506368 created "2021-12-31" @default.
- W4200506368 creator A5033767927 @default.
- W4200506368 creator A5036254574 @default.
- W4200506368 creator A5073975203 @default.
- W4200506368 creator A5084313840 @default.
- W4200506368 date "2021-12-03" @default.
- W4200506368 modified "2023-10-17" @default.
- W4200506368 title "Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia" @default.
- W4200506368 cites W1911515552 @default.
- W4200506368 cites W1992551495 @default.
- W4200506368 cites W2004548026 @default.
- W4200506368 cites W2008046969 @default.
- W4200506368 cites W2043036082 @default.
- W4200506368 cites W2043174271 @default.
- W4200506368 cites W2046795415 @default.
- W4200506368 cites W2059794305 @default.
- W4200506368 cites W2064214240 @default.
- W4200506368 cites W2064610693 @default.
- W4200506368 cites W2077764360 @default.
- W4200506368 cites W2087513475 @default.
- W4200506368 cites W2088317054 @default.
- W4200506368 cites W2114853012 @default.
- W4200506368 cites W2120108890 @default.
- W4200506368 cites W2124088653 @default.
- W4200506368 cites W2133707787 @default.
- W4200506368 cites W2142343447 @default.
- W4200506368 cites W2145069900 @default.
- W4200506368 cites W2146728678 @default.
- W4200506368 cites W2155802702 @default.
- W4200506368 cites W2156495516 @default.
- W4200506368 cites W2162408998 @default.
- W4200506368 cites W2162940218 @default.
- W4200506368 cites W2233510100 @default.
- W4200506368 cites W2235238101 @default.
- W4200506368 cites W2259283542 @default.
- W4200506368 cites W2393535458 @default.
- W4200506368 cites W2409517729 @default.
- W4200506368 cites W2443992611 @default.
- W4200506368 cites W2467323928 @default.
- W4200506368 cites W2470892681 @default.
- W4200506368 cites W2474887017 @default.
- W4200506368 cites W2561202938 @default.
- W4200506368 cites W2562044150 @default.
- W4200506368 cites W2612642053 @default.
- W4200506368 cites W2613205665 @default.
- W4200506368 cites W2614917539 @default.
- W4200506368 cites W2623963207 @default.
- W4200506368 cites W2725995969 @default.
- W4200506368 cites W2731127457 @default.
- W4200506368 cites W2741628575 @default.
- W4200506368 cites W2743296461 @default.
- W4200506368 cites W2748628637 @default.
- W4200506368 cites W2749301992 @default.
- W4200506368 cites W2751588083 @default.
- W4200506368 cites W2789366937 @default.
- W4200506368 cites W2793753655 @default.
- W4200506368 cites W2891902492 @default.
- W4200506368 cites W2892211216 @default.
- W4200506368 cites W2901036397 @default.
- W4200506368 cites W2911236522 @default.
- W4200506368 cites W2911362701 @default.
- W4200506368 cites W2923626201 @default.
- W4200506368 cites W2924109378 @default.
- W4200506368 cites W2924332698 @default.
- W4200506368 cites W2953305767 @default.
- W4200506368 cites W2953611986 @default.
- W4200506368 cites W2956957157 @default.
- W4200506368 cites W2963167594 @default.
- W4200506368 cites W2979075412 @default.
- W4200506368 cites W2979358136 @default.
- W4200506368 cites W2979416990 @default.
- W4200506368 cites W2982325569 @default.
- W4200506368 cites W2982810269 @default.
- W4200506368 cites W2983593436 @default.
- W4200506368 cites W2993639552 @default.
- W4200506368 cites W3007228197 @default.
- W4200506368 cites W3007431575 @default.
- W4200506368 cites W3009624086 @default.
- W4200506368 cites W3014262525 @default.
- W4200506368 cites W3023328519 @default.
- W4200506368 cites W3031591096 @default.
- W4200506368 cites W3045118558 @default.
- W4200506368 cites W3092937837 @default.
- W4200506368 cites W3130386497 @default.
- W4200506368 cites W3132606228 @default.
- W4200506368 cites W3135840475 @default.
- W4200506368 cites W3139237337 @default.
- W4200506368 cites W3143679334 @default.
- W4200506368 cites W3153485154 @default.
- W4200506368 cites W3156591468 @default.
- W4200506368 cites W3157105683 @default.
- W4200506368 cites W3165061606 @default.
- W4200506368 cites W3194630976 @default.
- W4200506368 doi "https://doi.org/10.3389/fmicb.2021.763136" @default.
- W4200506368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34925270" @default.
- W4200506368 hasPublicationYear "2021" @default.
- W4200506368 type Work @default.
- W4200506368 citedByCount "8" @default.
- W4200506368 countsByYear W42005063682022 @default.