Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200509905> ?p ?o ?g. }
- W4200509905 endingPage "1129" @default.
- W4200509905 startingPage "1119" @default.
- W4200509905 abstract "Bimolecular fluorescence complementation (BiFC) and its derivative molecular biosensor systems provide effective tools for visualizing biomolecular interactions. The introduction of red and near-infrared fluorescence emission proteins has expanded the spectrum of signal generating modules, enabling BiFC for in vivo imaging. However, the large size of the signal module of BiFC can hinder the interaction between proteins under investigation. In this study, we constructed the near-infrared BiFC and TriFC systems by splitting miRFP670nano, the smallest cyanobacteriochrome-evolved phytochrome available. The miRFP670nano-BiFC sensor system identified and enabled visualization of protein-protein interactions in living cells and live mice, and afforded a faster maturation rate and higher photostability and cellular stability when compared with those of reported near-infrared BiFC systems. We used the miRFP670nano-BiFC sensor system to identify interactions between the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and cellular stress granule proteins in living cells and found that the N protein downregulated the expression level of granule protein G3BP1. With the advantages of small size and long wavelength emission of the signal module, the proposed molecular biosensor system should be suitable for various applications in cell imaging studies." @default.
- W4200509905 created "2021-12-31" @default.
- W4200509905 creator A5017365002 @default.
- W4200509905 creator A5025719276 @default.
- W4200509905 creator A5065279811 @default.
- W4200509905 creator A5067228959 @default.
- W4200509905 date "2022-01-01" @default.
- W4200509905 modified "2023-10-13" @default.
- W4200509905 title "The smallest near-infrared fluorescence complementation system for imaging protein–protein and RNA–protein interactions" @default.
- W4200509905 cites W1034557034 @default.
- W4200509905 cites W1965169361 @default.
- W4200509905 cites W1972919132 @default.
- W4200509905 cites W1979451155 @default.
- W4200509905 cites W1980989869 @default.
- W4200509905 cites W2004872614 @default.
- W4200509905 cites W2011766905 @default.
- W4200509905 cites W2063559185 @default.
- W4200509905 cites W2088090032 @default.
- W4200509905 cites W2088335428 @default.
- W4200509905 cites W2097055018 @default.
- W4200509905 cites W2114506550 @default.
- W4200509905 cites W2123924700 @default.
- W4200509905 cites W2124304575 @default.
- W4200509905 cites W2128707434 @default.
- W4200509905 cites W2148542126 @default.
- W4200509905 cites W2508704470 @default.
- W4200509905 cites W2885573416 @default.
- W4200509905 cites W2886432125 @default.
- W4200509905 cites W2898199658 @default.
- W4200509905 cites W2911513195 @default.
- W4200509905 cites W2971991951 @default.
- W4200509905 cites W3004280078 @default.
- W4200509905 cites W3036339225 @default.
- W4200509905 cites W3107526275 @default.
- W4200509905 cites W3110571029 @default.
- W4200509905 cites W3125800928 @default.
- W4200509905 cites W3136830552 @default.
- W4200509905 cites W3143867892 @default.
- W4200509905 cites W3152577942 @default.
- W4200509905 cites W3163180626 @default.
- W4200509905 cites W3179194243 @default.
- W4200509905 cites W3204581885 @default.
- W4200509905 doi "https://doi.org/10.1039/d1sc04839b" @default.
- W4200509905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35211278" @default.
- W4200509905 hasPublicationYear "2022" @default.
- W4200509905 type Work @default.
- W4200509905 citedByCount "4" @default.
- W4200509905 countsByYear W42005099052022 @default.
- W4200509905 countsByYear W42005099052023 @default.
- W4200509905 crossrefType "journal-article" @default.
- W4200509905 hasAuthorship W4200509905A5017365002 @default.
- W4200509905 hasAuthorship W4200509905A5025719276 @default.
- W4200509905 hasAuthorship W4200509905A5065279811 @default.
- W4200509905 hasAuthorship W4200509905A5067228959 @default.
- W4200509905 hasBestOaLocation W42005099051 @default.
- W4200509905 hasConcept C104317684 @default.
- W4200509905 hasConcept C11804247 @default.
- W4200509905 hasConcept C120665830 @default.
- W4200509905 hasConcept C121332964 @default.
- W4200509905 hasConcept C123243835 @default.
- W4200509905 hasConcept C12554922 @default.
- W4200509905 hasConcept C142613039 @default.
- W4200509905 hasConcept C143065580 @default.
- W4200509905 hasConcept C158355884 @default.
- W4200509905 hasConcept C160756335 @default.
- W4200509905 hasConcept C171250308 @default.
- W4200509905 hasConcept C185592680 @default.
- W4200509905 hasConcept C188082640 @default.
- W4200509905 hasConcept C192562407 @default.
- W4200509905 hasConcept C55493867 @default.
- W4200509905 hasConcept C86803240 @default.
- W4200509905 hasConcept C91881484 @default.
- W4200509905 hasConcept C95444343 @default.
- W4200509905 hasConceptScore W4200509905C104317684 @default.
- W4200509905 hasConceptScore W4200509905C11804247 @default.
- W4200509905 hasConceptScore W4200509905C120665830 @default.
- W4200509905 hasConceptScore W4200509905C121332964 @default.
- W4200509905 hasConceptScore W4200509905C123243835 @default.
- W4200509905 hasConceptScore W4200509905C12554922 @default.
- W4200509905 hasConceptScore W4200509905C142613039 @default.
- W4200509905 hasConceptScore W4200509905C143065580 @default.
- W4200509905 hasConceptScore W4200509905C158355884 @default.
- W4200509905 hasConceptScore W4200509905C160756335 @default.
- W4200509905 hasConceptScore W4200509905C171250308 @default.
- W4200509905 hasConceptScore W4200509905C185592680 @default.
- W4200509905 hasConceptScore W4200509905C188082640 @default.
- W4200509905 hasConceptScore W4200509905C192562407 @default.
- W4200509905 hasConceptScore W4200509905C55493867 @default.
- W4200509905 hasConceptScore W4200509905C86803240 @default.
- W4200509905 hasConceptScore W4200509905C91881484 @default.
- W4200509905 hasConceptScore W4200509905C95444343 @default.
- W4200509905 hasFunder F4320321001 @default.
- W4200509905 hasFunder F4320321133 @default.
- W4200509905 hasFunder F4320335777 @default.
- W4200509905 hasFunder F4320337111 @default.
- W4200509905 hasIssue "4" @default.
- W4200509905 hasLocation W42005099051 @default.
- W4200509905 hasLocation W42005099052 @default.