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• W4200511606 endingPage "133382" @default.
• W4200511606 startingPage "133382" @default.
• W4200511606 abstract "This study aims to present a simple and effective carrier matrix to immobilize laccase as opposed to complex and tedious immobilization processes and also to use it in the removal of textile dyes. For this purpose, Cobalt (Co) and Copper (Cu) based metal-organic frameworks (MOFs) were prepared and laccase was immobilized on two different MOFs via encapsulation. The characterization outcomes showed that laccase was well immobilized into MOF supports. Optimum pH and temperature were found for Lac/Co-MOF (pH 4.5 at 50 °C) and Lac/Cu-MOF (pH 5.0 at 50 °C). The Km (0.03 mM) and Vmax (97.4 μmol/min) values of Lac/Cu-MOF were lower than those of Lac/Co-MOF (Km = 0.13 mM, Vmax = 230.7 μmol/min). The immobilized laccases showed good reusability as well as improved resistance to temperature denaturation and high storage stability. For instance, the Lac/Co-MOF and Lac/Cu-MOF retained more than 58% activity after 4 weeks of storage at room temperature. Meanwhile, Lac/Co-MOF and Lac/Cu-MOF maintained 56.5% and 55.8% of their initial activity, respectively, after 12 reuse cycles. Moreover, thermal deactivation kinetic studies of immobilized laccases displayed lower k value, higher t1/2, and enhancement of thermodynamic parameters, which means better thermostability. Finally, the decolorization activities for the Lac/Co-MOF were 78% and 61% at the 5th cycle for Reactive Blue 171 and Reactive Blue 198, respectively. In conclusion, it can be inferred that the MOFs are more sustainable and beneficial support for laccase immobilization and they can be efficient for removing textile dyes from industrial wastes." @default.
• W4200511606 created "2021-12-31" @default.
• W4200511606 creator A5013155195 @default.
• W4200511606 creator A5013587360 @default.
• W4200511606 creator A5023620386 @default.
• W4200511606 creator A5059885776 @default.
• W4200511606 creator A5085081128 @default.
• W4200511606 creator A5086489032 @default.
• W4200511606 date "2022-04-01" @default.
• W4200511606 modified "2023-10-18" @default.
• W4200511606 title "Design of laccase–metal–organic framework hybrid constructs for biocatalytic removal of textile dyes" @default.
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• W4200511606 doi "https://doi.org/10.1016/j.chemosphere.2021.133382" @default.

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