Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200517103> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W4200517103 abstract "Turbidimetry is a laboratory technique that is applied to measure the aggregation of platelets suspended in either plasma (platelet-rich plasma, PRP) or in buffer (washed platelets), by the use of one or a combination of agonists. The use of washed platelets separated from their plasma environment and in the absence of anticoagulants allows for studying intrinsic platelet properties. Among the large panel of agonists, arachidonic acid (AA), adenosine di-phosphate (ADP), thrombin and collagen are the most frequently used. The aggregation response is quantified by measuring the relative optical density (OD) over time of platelet suspension under continuous stirring. Platelets in homogeneous suspension limit the passage of light after the addition of an agonist, platelet shape change occurs producing a small transitory increase in OD. Following this initial activation step, platelet clots form gradually, allowing the passage of light through the suspension as a result of decreased OD. The aggregation process is ultimately expressed as a percentage, compared to the OD of platelet-poor plasma or buffer. Rigorous calibration is thus essential at the beginning of each experiment. As a general rule: calibration to 0% is set by measuring the OD of a non-stimulated platelet suspension while measuring the OD of the suspension medium containing no platelets represents a value of 100%. Platelet aggregation is generally visualized as a real-time aggregation curve. Turbidimetry is one of the most commonly used laboratory techniques for the investigation of platelet function and is considered as the historical gold standard and used for the development of new pharmaceutical agents aimed at inhibiting platelet aggregation. Here, we describe detailed protocols for 1) preparation of human washed platelets and 2) turbidimetric analysis of collagen-induced aggregation of human washed platelets pretreated with the food dye Brilliant Blue FCF that was recently identified as an inhibitor of Pannexin1 (Panx1) channels." @default.
- W4200517103 created "2021-12-31" @default.
- W4200517103 creator A5005019343 @default.
- W4200517103 creator A5007297824 @default.
- W4200517103 creator A5059511936 @default.
- W4200517103 creator A5076412551 @default.
- W4200517103 date "2017-04-06" @default.
- W4200517103 modified "2023-10-16" @default.
- W4200517103 title "Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation" @default.
- W4200517103 doi "https://doi.org/10.3791/55525-v" @default.
- W4200517103 hasPublicationYear "2017" @default.
- W4200517103 type Work @default.
- W4200517103 citedByCount "0" @default.
- W4200517103 crossrefType "journal-article" @default.
- W4200517103 hasAuthorship W4200517103A5005019343 @default.
- W4200517103 hasAuthorship W4200517103A5007297824 @default.
- W4200517103 hasAuthorship W4200517103A5059511936 @default.
- W4200517103 hasAuthorship W4200517103A5076412551 @default.
- W4200517103 hasBestOaLocation W42005171032 @default.
- W4200517103 hasConcept C105341887 @default.
- W4200517103 hasConcept C12554922 @default.
- W4200517103 hasConcept C185592680 @default.
- W4200517103 hasConcept C202444582 @default.
- W4200517103 hasConcept C203014093 @default.
- W4200517103 hasConcept C2777292125 @default.
- W4200517103 hasConcept C2777399203 @default.
- W4200517103 hasConcept C2778914503 @default.
- W4200517103 hasConcept C2780076745 @default.
- W4200517103 hasConcept C2780777798 @default.
- W4200517103 hasConcept C2993802102 @default.
- W4200517103 hasConcept C33923547 @default.
- W4200517103 hasConcept C43617362 @default.
- W4200517103 hasConcept C5961521 @default.
- W4200517103 hasConcept C86803240 @default.
- W4200517103 hasConcept C89560881 @default.
- W4200517103 hasConceptScore W4200517103C105341887 @default.
- W4200517103 hasConceptScore W4200517103C12554922 @default.
- W4200517103 hasConceptScore W4200517103C185592680 @default.
- W4200517103 hasConceptScore W4200517103C202444582 @default.
- W4200517103 hasConceptScore W4200517103C203014093 @default.
- W4200517103 hasConceptScore W4200517103C2777292125 @default.
- W4200517103 hasConceptScore W4200517103C2777399203 @default.
- W4200517103 hasConceptScore W4200517103C2778914503 @default.
- W4200517103 hasConceptScore W4200517103C2780076745 @default.
- W4200517103 hasConceptScore W4200517103C2780777798 @default.
- W4200517103 hasConceptScore W4200517103C2993802102 @default.
- W4200517103 hasConceptScore W4200517103C33923547 @default.
- W4200517103 hasConceptScore W4200517103C43617362 @default.
- W4200517103 hasConceptScore W4200517103C5961521 @default.
- W4200517103 hasConceptScore W4200517103C86803240 @default.
- W4200517103 hasConceptScore W4200517103C89560881 @default.
- W4200517103 hasIssue "122" @default.
- W4200517103 hasLocation W42005171031 @default.
- W4200517103 hasLocation W42005171032 @default.
- W4200517103 hasOpenAccess W4200517103 @default.
- W4200517103 hasPrimaryLocation W42005171031 @default.
- W4200517103 hasRelatedWork W1969065772 @default.
- W4200517103 hasRelatedWork W1977136153 @default.
- W4200517103 hasRelatedWork W2056880539 @default.
- W4200517103 hasRelatedWork W2415339342 @default.
- W4200517103 hasRelatedWork W2418262560 @default.
- W4200517103 hasRelatedWork W2472963372 @default.
- W4200517103 hasRelatedWork W25390149 @default.
- W4200517103 hasRelatedWork W2605284420 @default.
- W4200517103 hasRelatedWork W2622472364 @default.
- W4200517103 hasRelatedWork W2804680820 @default.
- W4200517103 isParatext "false" @default.
- W4200517103 isRetracted "false" @default.
- W4200517103 workType "article" @default.