FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200523890> ?p ?o ?g. }

• W4200523890 abstract "The various advantages associated with the growth properties of Escherichia coli have justified their use in the production of genetically engineered vaccines. However, endotoxin contamination, plasmid vector instability, and the requirement for antibiotic supplementation are frequent bottlenecks in the successful production of recombinant proteins that are safe for industrial-scaled applications. To overcome these drawbacks, we focused on interrupting the expression of several key genes involved in the synthesis of lipopolysaccharide (LPS), an endotoxin frequently responsible for toxicity in recombinant proteins, to eliminate endotoxin contamination and produce better recombinant proteins with E. coli.Of 8 potential target genes associated with LPS synthesis, we successfully constructed 7 LPS biosynthesis-defective recombinant strains to reduce the production of LPS. The endotoxin residue in the protein products from these modified E. coli strains were about two orders of magnitude lower than that produced by the wild-type strain. Further, we found that 6 loci-lpxM, lpxP, lpxL, eptA, gutQ and kdsD-were suitable for chromosomal integrated expression of HPV L1 protein. We found that a single copy of the expression cassette conferred stable expression during long-term antibiotic-free cultivation as compared with the more variable protein production from plasmid-based expression. In large-scale fermentation, we found that recombinant strains bearing 3 to 5 copies of the expression cassette had 1.5- to 2-fold higher overall expression along with lower endotoxin levels as compared with the parental ER2566 strain. Finally, we engineered and constructed 9 recombinant E. coli strains for the later production of an HPV 9-valent capsid protein with desirable purity, VLP morphology, and antigenicity.Reengineering the LPS synthesis loci in the E. coli ER2566 strain through chromosomal integration of expression cassettes has potential uses for the production of a 9-valent HPV vaccine candidate, with markedly reduced residual endotoxin levels. Our results offer a new strategy for recombinant E. coli strain construction, engineering, and the development of suitable recombinant protein drugs." @default.
• W4200523890 created "2021-12-31" @default.
• W4200523890 creator A5005053576 @default.
• W4200523890 creator A5005358283 @default.
• W4200523890 creator A5010142228 @default.
• W4200523890 creator A5014283454 @default.
• W4200523890 creator A5017384752 @default.
• W4200523890 creator A5018093574 @default.
• W4200523890 creator A5024479009 @default.
• W4200523890 creator A5038086854 @default.
• W4200523890 creator A5041799888 @default.
• W4200523890 creator A5051881032 @default.
• W4200523890 creator A5067654958 @default.
• W4200523890 creator A5070168366 @default.
• W4200523890 creator A5076030734 @default.
• W4200523890 creator A5077554152 @default.
• W4200523890 creator A5078210046 @default.
• W4200523890 creator A5078732636 @default.
• W4200523890 creator A5085399047 @default.
• W4200523890 date "2021-12-01" @default.
• W4200523890 modified "2023-10-15" @default.
• W4200523890 title "Engineering for an HPV 9-valent vaccine candidate using genomic constitutive over-expression and low lipopolysaccharide levels in Escherichia coli cells" @default.
• W4200523890 cites W1553348964 @default.
• W4200523890 cites W1963674925 @default.
• W4200523890 cites W1967245701 @default.
• W4200523890 cites W1973432832 @default.
• W4200523890 cites W1985677799 @default.
• W4200523890 cites W1990577248 @default.
• W4200523890 cites W1992540782 @default.
• W4200523890 cites W2008487520 @default.
• W4200523890 cites W2024622044 @default.
• W4200523890 cites W2026754153 @default.
• W4200523890 cites W2027216673 @default.
• W4200523890 cites W2040941644 @default.
• W4200523890 cites W2041740586 @default.
• W4200523890 cites W2060873330 @default.
• W4200523890 cites W2063787905 @default.
• W4200523890 cites W2072461975 @default.
• W4200523890 cites W2115419697 @default.
• W4200523890 cites W2132960606 @default.
• W4200523890 cites W2138820253 @default.
• W4200523890 cites W2152413278 @default.
• W4200523890 cites W2153492724 @default.
• W4200523890 cites W2159826228 @default.
• W4200523890 cites W2159971058 @default.
• W4200523890 cites W2319351713 @default.
• W4200523890 cites W2610576462 @default.
• W4200523890 cites W2893560272 @default.
• W4200523890 cites W2945441427 @default.
• W4200523890 cites W3036647629 @default.
• W4200523890 cites W3045460309 @default.
• W4200523890 cites W3048484676 @default.
• W4200523890 cites W82178089 @default.
• W4200523890 doi "https://doi.org/10.1186/s12934-021-01719-8" @default.
• W4200523890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34930257" @default.
• W4200523890 hasPublicationYear "2021" @default.
• W4200523890 type Work @default.
• W4200523890 citedByCount "4" @default.
• W4200523890 countsByYear W42005238902022 @default.
• W4200523890 countsByYear W42005238902023 @default.
• W4200523890 crossrefType "journal-article" @default.
• W4200523890 hasAuthorship W4200523890A5005053576 @default.
• W4200523890 hasAuthorship W4200523890A5005358283 @default.
• W4200523890 hasAuthorship W4200523890A5010142228 @default.
• W4200523890 hasAuthorship W4200523890A5014283454 @default.
• W4200523890 hasAuthorship W4200523890A5017384752 @default.
• W4200523890 hasAuthorship W4200523890A5018093574 @default.
• W4200523890 hasAuthorship W4200523890A5024479009 @default.
• W4200523890 hasAuthorship W4200523890A5038086854 @default.
• W4200523890 hasAuthorship W4200523890A5041799888 @default.
• W4200523890 hasAuthorship W4200523890A5051881032 @default.
• W4200523890 hasAuthorship W4200523890A5067654958 @default.
• W4200523890 hasAuthorship W4200523890A5070168366 @default.
• W4200523890 hasAuthorship W4200523890A5076030734 @default.
• W4200523890 hasAuthorship W4200523890A5077554152 @default.
• W4200523890 hasAuthorship W4200523890A5078210046 @default.
• W4200523890 hasAuthorship W4200523890A5078732636 @default.
• W4200523890 hasAuthorship W4200523890A5085399047 @default.
• W4200523890 hasBestOaLocation W42005238901 @default.
• W4200523890 hasConcept C100012643 @default.
• W4200523890 hasConcept C104317684 @default.
• W4200523890 hasConcept C134018914 @default.
• W4200523890 hasConcept C22744801 @default.
• W4200523890 hasConcept C2778754761 @default.
• W4200523890 hasConcept C33161422 @default.
• W4200523890 hasConcept C40767141 @default.
• W4200523890 hasConcept C54355233 @default.
• W4200523890 hasConcept C547475151 @default.
• W4200523890 hasConcept C86803240 @default.
• W4200523890 hasConcept C89423630 @default.
• W4200523890 hasConcept C92087593 @default.
• W4200523890 hasConceptScore W4200523890C100012643 @default.
• W4200523890 hasConceptScore W4200523890C104317684 @default.
• W4200523890 hasConceptScore W4200523890C134018914 @default.
• W4200523890 hasConceptScore W4200523890C22744801 @default.
• W4200523890 hasConceptScore W4200523890C2778754761 @default.
• W4200523890 hasConceptScore W4200523890C33161422 @default.
• W4200523890 hasConceptScore W4200523890C40767141 @default.
• W4200523890 hasConceptScore W4200523890C54355233 @default.
• W4200523890 hasConceptScore W4200523890C547475151 @default.

• next