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- W4200524144 abstract "The purpose of this study was to identify proteins that regulate vascular remodeling in an ROP mouse model. Pups were subjected to fluctuating oxygen levels and retinas sampled during vessel regression (PN12) or neovascularization (PN17) for comparative SWATH-MS proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We developed a human retinal endothelial cell (HREC) ROP correlate to validate the expression of retina neovascular-specific markers. A total of 5191 proteins were identified in OIR retinas with 498 significantly regulated in elevated oxygen and 345 after a return to normoxia. A total of 122 proteins were uniquely regulated during vessel regression and 69 during neovascularization (FC ≥ 1.5; p ≤ 0.05), with several validated by western blot analyses. Expressions of 56/69 neovascular-specific proteins were confirmed in hypoxic HRECs with 23 regulated in the same direction as OIR neovascular retinas. These proteins control angiogenesis-related processes including matrix remodeling, cell migration, adhesion, and proliferation. RNAi and transfection overexpression studies confirmed that VASP and ECH1, showing the highest levels in hypoxic HRECs, promoted human umbilical vein (HUVEC) and HREC cell proliferation, while SNX1 and CD109, showing the lowest levels, inhibited their proliferation. These proteins are potential biomarkers and exploitable intervention tools for vascular-related disorders. The proteomics data set generated has been deposited to the ProteomeXchange/iProX Consortium with the Identifier:PXD029208." @default.
- W4200524144 created "2021-12-31" @default.
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- W4200524144 date "2021-12-17" @default.
- W4200524144 modified "2023-09-24" @default.
- W4200524144 title "Novel Regulators of Retina Neovascularization: A Proteomics Approach" @default.
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- W4200524144 cites W1967004211 @default.
- W4200524144 cites W1977532673 @default.
- W4200524144 cites W1988169813 @default.
- W4200524144 cites W1990555365 @default.
- W4200524144 cites W1995340584 @default.
- W4200524144 cites W1998726711 @default.
- W4200524144 cites W2000099545 @default.
- W4200524144 cites W2001884742 @default.
- W4200524144 cites W2003786504 @default.
- W4200524144 cites W2003855294 @default.
- W4200524144 cites W2009399774 @default.
- W4200524144 cites W2013809269 @default.
- W4200524144 cites W2015920746 @default.
- W4200524144 cites W2016203088 @default.
- W4200524144 cites W2022084143 @default.
- W4200524144 cites W2037517553 @default.
- W4200524144 cites W2040125739 @default.
- W4200524144 cites W2053270619 @default.
- W4200524144 cites W2067194066 @default.
- W4200524144 cites W2072897672 @default.
- W4200524144 cites W2080627917 @default.
- W4200524144 cites W2086080780 @default.
- W4200524144 cites W2092104135 @default.
- W4200524144 cites W2093915706 @default.
- W4200524144 cites W2094844827 @default.
- W4200524144 cites W2106868003 @default.
- W4200524144 cites W2134467433 @default.
- W4200524144 cites W2135713425 @default.
- W4200524144 cites W2139761967 @default.
- W4200524144 cites W2141816935 @default.
- W4200524144 cites W2147966608 @default.
- W4200524144 cites W2155628054 @default.
- W4200524144 cites W2155645904 @default.
- W4200524144 cites W2168097635 @default.
- W4200524144 cites W2169632112 @default.
- W4200524144 cites W2293662776 @default.
- W4200524144 cites W2324539199 @default.
- W4200524144 cites W2340928134 @default.
- W4200524144 cites W2385884905 @default.
- W4200524144 cites W2520941279 @default.
- W4200524144 cites W2582367431 @default.
- W4200524144 cites W2601431472 @default.
- W4200524144 cites W2611061209 @default.
- W4200524144 cites W2726623879 @default.
- W4200524144 cites W2765972177 @default.
- W4200524144 cites W2767329860 @default.
- W4200524144 cites W2769102191 @default.
- W4200524144 cites W2778419227 @default.
- W4200524144 cites W2789587888 @default.
- W4200524144 cites W2789680594 @default.
- W4200524144 cites W2792913738 @default.
- W4200524144 cites W2796311674 @default.
- W4200524144 cites W2796597850 @default.
- W4200524144 cites W2805262141 @default.
- W4200524144 cites W2876744403 @default.
- W4200524144 cites W2883673917 @default.
- W4200524144 cites W2889463119 @default.
- W4200524144 cites W2889846670 @default.
- W4200524144 cites W2893186772 @default.
- W4200524144 cites W2910656364 @default.
- W4200524144 cites W2912781730 @default.
- W4200524144 cites W2940407405 @default.
- W4200524144 cites W2957575643 @default.
- W4200524144 cites W2957854257 @default.
- W4200524144 cites W2996184770 @default.
- W4200524144 cites W2998498622 @default.
- W4200524144 cites W3008851651 @default.
- W4200524144 cites W3009956843 @default.
- W4200524144 cites W3010072286 @default.
- W4200524144 cites W3021782557 @default.
- W4200524144 cites W3025363008 @default.
- W4200524144 cites W3081187361 @default.
- W4200524144 cites W3089709611 @default.
- W4200524144 cites W3092360522 @default.
- W4200524144 cites W4252028598 @default.
- W4200524144 doi "https://doi.org/10.1021/acs.jproteome.1c00547" @default.
- W4200524144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34919406" @default.
- W4200524144 hasPublicationYear "2021" @default.
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