Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200527480> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W4200527480 endingPage "39" @default.
- W4200527480 startingPage "31" @default.
- W4200527480 abstract "
 
 Pancreatitis-associated protein (PAP) is a pancreatic stress protein that is not produced in a healthy pancreas but is highly synthesized in pancreatic acinar cells in response to acute and chronic pancreatitis, hypoxia, toxins, diabetes, lipopolysaccharides hypotransferrinemia and organ transplantation. Changes in the PAP levels in serum are an important biological marker in the early stage of pancreatic diseases. In this study, the recombinant human PAP protein, which has the potential to be used as a diagnostic marker and as research material in proliferation, apoptosis, cell migration, cell invasion, and immunoassay studies, was expressed efficiently under the control of the AOX1 gene promoter in the Komagataella phaffii (Pichia pastoris) (K. phaffii) X33 strain. We describe the conditions required for the efficient production of PAP protein by methanol induction and its use without purification. The produced unpurified protein was tested in sandwich ELISA and showed consistent results with the commercial product. These results are encouraging that the protein produced can be used as a biomarker standard in ELISA tests without the cost and labor of purification.
 
 
 
 
" @default.
- W4200527480 created "2021-12-31" @default.
- W4200527480 creator A5030949274 @default.
- W4200527480 creator A5040869022 @default.
- W4200527480 creator A5067119354 @default.
- W4200527480 creator A5091551936 @default.
- W4200527480 date "2021-12-28" @default.
- W4200527480 modified "2023-10-16" @default.
- W4200527480 title "Production of Human Pancreatitis-Associated Protein (hPAP) in Komagataella phaffii (Pichia pastoris)" @default.
- W4200527480 doi "https://doi.org/10.31383/ga.vol5iss2pp31-39" @default.
- W4200527480 hasPublicationYear "2021" @default.
- W4200527480 type Work @default.
- W4200527480 citedByCount "0" @default.
- W4200527480 crossrefType "journal-article" @default.
- W4200527480 hasAuthorship W4200527480A5030949274 @default.
- W4200527480 hasAuthorship W4200527480A5040869022 @default.
- W4200527480 hasAuthorship W4200527480A5067119354 @default.
- W4200527480 hasAuthorship W4200527480A5091551936 @default.
- W4200527480 hasBestOaLocation W42005274801 @default.
- W4200527480 hasConcept C104317684 @default.
- W4200527480 hasConcept C126322002 @default.
- W4200527480 hasConcept C159654299 @default.
- W4200527480 hasConcept C203014093 @default.
- W4200527480 hasConcept C2775967933 @default.
- W4200527480 hasConcept C2779703480 @default.
- W4200527480 hasConcept C2780420688 @default.
- W4200527480 hasConcept C2781372317 @default.
- W4200527480 hasConcept C40767141 @default.
- W4200527480 hasConcept C55493867 @default.
- W4200527480 hasConcept C71924100 @default.
- W4200527480 hasConcept C86803240 @default.
- W4200527480 hasConceptScore W4200527480C104317684 @default.
- W4200527480 hasConceptScore W4200527480C126322002 @default.
- W4200527480 hasConceptScore W4200527480C159654299 @default.
- W4200527480 hasConceptScore W4200527480C203014093 @default.
- W4200527480 hasConceptScore W4200527480C2775967933 @default.
- W4200527480 hasConceptScore W4200527480C2779703480 @default.
- W4200527480 hasConceptScore W4200527480C2780420688 @default.
- W4200527480 hasConceptScore W4200527480C2781372317 @default.
- W4200527480 hasConceptScore W4200527480C40767141 @default.
- W4200527480 hasConceptScore W4200527480C55493867 @default.
- W4200527480 hasConceptScore W4200527480C71924100 @default.
- W4200527480 hasConceptScore W4200527480C86803240 @default.
- W4200527480 hasIssue "2" @default.
- W4200527480 hasLocation W42005274801 @default.
- W4200527480 hasOpenAccess W4200527480 @default.
- W4200527480 hasPrimaryLocation W42005274801 @default.
- W4200527480 hasRelatedWork W2348094644 @default.
- W4200527480 hasRelatedWork W2363862718 @default.
- W4200527480 hasRelatedWork W2365855640 @default.
- W4200527480 hasRelatedWork W2368039950 @default.
- W4200527480 hasRelatedWork W2372962808 @default.
- W4200527480 hasRelatedWork W2374357896 @default.
- W4200527480 hasRelatedWork W2381034741 @default.
- W4200527480 hasRelatedWork W2383850870 @default.
- W4200527480 hasRelatedWork W2388679795 @default.
- W4200527480 hasRelatedWork W2912153741 @default.
- W4200527480 hasVolume "5" @default.
- W4200527480 isParatext "false" @default.
- W4200527480 isRetracted "false" @default.
- W4200527480 workType "article" @default.