FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200528099> ?p ?o ?g. }

• W4200528099 abstract "Abstract Nucleoli have viscoelastic gel-like condensate dynamics that are not well represented in vitro . Nucleoli models, such as those formed by nucleophosmin 1 (NPM1) and ribosomal RNA (rRNA), exhibit condensate dynamics orders of magnitude faster than in vivo nucleoli. Here we show that an interplay between magnesium ions (Mg 2+ ) and ATP governs rRNA dynamics, and this ultimately shapes the physical state of these condensates. Using quantitative fluorescence microscopy, we demonstrate that increased RNA compaction occurs in the condensates at high Mg 2+ concentrations, contributing to the slowed RNA dynamics. At Mg 2+ concentrations above 7 mM, rRNA is fully arrested and the condensates are gels. Below the critical gel point, NPM1-rRNA droplets age in a temperature-dependent manner, suggesting that condensates are viscoelastic materials, undergoing maturation driven by weak multivalent interactions. ATP addition reverses the dynamic arrest of rRNA, resulting in liquefaction of these gel-like structures. Surprisingly, ATP and Mg 2+ both act to increase partitioning of NPM1-proteins as well as rRNA, which influences the partitioning of small client molecules. By contrast, larger ribosomes form a halo around NPM1-rRNA coacervates when Mg 2+ concentrations are higher than ATP concentrations. Within cells, ATP levels fluctuate due to biomolecular reactions, and we demonstrate that a dissipative enzymatic reaction can control the biophysical properties of in vitro condensates through depletion of ATP. This enzymatic ATP depletion also reverses the formation of the ribosome halos. Our results illustrate how cells, by changing local ATP concentrations, may regulate the state and client partitioning of RNA-containing condensates such as the nucleolus. Significance Statement There is a significant discrepancy between the dynamics of in vitro nucleolus models and in vivo nucleoli – with the latter more gel-like. The interplay between Mg 2+ ions, ATP and the nucleolus components – specifically RNA – governs the dynamics, and ultimately the physical state, of nucleolus-like condensates. We show that the nucleolus are dynamically adapting condensates, responding to local ATP concentrations through Mg 2+ -induced compaction of the RNA, and reversible relaxation when ATP binds Mg 2+ again. Other condensates containing RNA probably respond in similar ways to Mg 2+ and ATP." @default.
• W4200528099 created "2021-12-31" @default.
• W4200528099 creator A5002172742 @default.
• W4200528099 creator A5013089775 @default.
• W4200528099 creator A5022792563 @default.
• W4200528099 creator A5029236232 @default.
• W4200528099 creator A5055835273 @default.
• W4200528099 creator A5073784581 @default.
• W4200528099 date "2021-12-22" @default.
• W4200528099 modified "2023-09-25" @default.
• W4200528099 title "ATP:Mg²⁺shapes condensate properties of rRNA-NPM1<i>in vitro</i>nucleolus model and its partitioning of ribosomes" @default.
• W4200528099 cites W1587454301 @default.
• W4200528099 cites W1977754603 @default.
• W4200528099 cites W1991753500 @default.
• W4200528099 cites W2001366340 @default.
• W4200528099 cites W2002373110 @default.
• W4200528099 cites W2026226960 @default.
• W4200528099 cites W2044939547 @default.
• W4200528099 cites W2075990344 @default.
• W4200528099 cites W2095032945 @default.
• W4200528099 cites W2110884351 @default.
• W4200528099 cites W2121997805 @default.
• W4200528099 cites W2129103509 @default.
• W4200528099 cites W2133059825 @default.
• W4200528099 cites W2255386862 @default.
• W4200528099 cites W2402791934 @default.
• W4200528099 cites W2412222674 @default.
• W4200528099 cites W2580785886 @default.
• W4200528099 cites W2783093261 @default.
• W4200528099 cites W2883179995 @default.
• W4200528099 cites W2905220927 @default.
• W4200528099 cites W2944659339 @default.
• W4200528099 cites W2947457847 @default.
• W4200528099 cites W2966973269 @default.
• W4200528099 cites W2970894940 @default.
• W4200528099 cites W2975526474 @default.
• W4200528099 cites W2989899924 @default.
• W4200528099 cites W3003193083 @default.
• W4200528099 cites W3016762840 @default.
• W4200528099 cites W3023602638 @default.
• W4200528099 cites W3082518512 @default.
• W4200528099 cites W3088252609 @default.
• W4200528099 cites W3090576304 @default.
• W4200528099 cites W3092577799 @default.
• W4200528099 cites W3096113641 @default.
• W4200528099 cites W3099738888 @default.
• W4200528099 cites W3105805391 @default.
• W4200528099 cites W3135083938 @default.
• W4200528099 cites W3135569509 @default.
• W4200528099 cites W3159964245 @default.
• W4200528099 cites W3171549870 @default.
• W4200528099 cites W3183669650 @default.
• W4200528099 cites W3198816848 @default.
• W4200528099 cites W3217333932 @default.
• W4200528099 cites W4206830860 @default.
• W4200528099 cites W4211158994 @default.
• W4200528099 doi "https://doi.org/10.1101/2021.12.22.473778" @default.
• W4200528099 hasPublicationYear "2021" @default.
• W4200528099 type Work @default.
• W4200528099 citedByCount "2" @default.
• W4200528099 countsByYear W42005280992022 @default.
• W4200528099 crossrefType "posted-content" @default.
• W4200528099 hasAuthorship W4200528099A5002172742 @default.
• W4200528099 hasAuthorship W4200528099A5013089775 @default.
• W4200528099 hasAuthorship W4200528099A5022792563 @default.
• W4200528099 hasAuthorship W4200528099A5029236232 @default.
• W4200528099 hasAuthorship W4200528099A5055835273 @default.
• W4200528099 hasAuthorship W4200528099A5073784581 @default.
• W4200528099 hasBestOaLocation W42005280991 @default.
• W4200528099 hasConcept C104317684 @default.
• W4200528099 hasConcept C10879258 @default.
• W4200528099 hasConcept C12554922 @default.
• W4200528099 hasConcept C183873130 @default.
• W4200528099 hasConcept C185592680 @default.
• W4200528099 hasConcept C190062978 @default.
• W4200528099 hasConcept C202751555 @default.
• W4200528099 hasConcept C55493867 @default.
• W4200528099 hasConcept C67705224 @default.
• W4200528099 hasConcept C67905577 @default.
• W4200528099 hasConcept C86803240 @default.
• W4200528099 hasConcept C88478588 @default.
• W4200528099 hasConcept C95444343 @default.
• W4200528099 hasConceptScore W4200528099C104317684 @default.
• W4200528099 hasConceptScore W4200528099C10879258 @default.
• W4200528099 hasConceptScore W4200528099C12554922 @default.
• W4200528099 hasConceptScore W4200528099C183873130 @default.
• W4200528099 hasConceptScore W4200528099C185592680 @default.
• W4200528099 hasConceptScore W4200528099C190062978 @default.
• W4200528099 hasConceptScore W4200528099C202751555 @default.
• W4200528099 hasConceptScore W4200528099C55493867 @default.
• W4200528099 hasConceptScore W4200528099C67705224 @default.
• W4200528099 hasConceptScore W4200528099C67905577 @default.
• W4200528099 hasConceptScore W4200528099C86803240 @default.
• W4200528099 hasConceptScore W4200528099C88478588 @default.
• W4200528099 hasConceptScore W4200528099C95444343 @default.
• W4200528099 hasLocation W42005280991 @default.
• W4200528099 hasOpenAccess W4200528099 @default.
• W4200528099 hasPrimaryLocation W42005280991 @default.
• W4200528099 hasRelatedWork W1526101099 @default.
• W4200528099 hasRelatedWork W1600443302 @default.

• next