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- W4200529156 abstract "Aldehyde dehydrogenases (ALDH), found in all kingdoms of life, form a superfamily of enzymes that primarily catalyse the oxidation of aldehydes to form carboxylic acid products, while utilising the cofactor NAD(P)+. Some superfamily members can also act as esterases using p-nitrophenyl esters as substrates. The ALDHTt from Thermus thermophilus was recombinantly expressed in E. coli and purified to obtain high yields (approximately 15-20 mg/L) and purity utilising an efficient heat treatment step coupled with IMAC and gel filtration chromatography. The use of the heat treatment step proved critical, in its absence decreased yield of 40% was observed. Characterisation of the thermophilic ALDHTt led to optimum enzymatic working conditions of 50 °C, and a pH of 8. ALDHTt possesses dual enzymatic activity, with the ability to act as a dehydrogenase and an esterase. ALDHTt possesses broad substrate specificity, displaying activity for a range of aldehydes, most notably hexanal and the synthetic dialdehyde, terephthalaldehyde. Interestingly, para-substituted benzaldehydes could be processed efficiently, but ortho-substitution resulted in no catalytic activity. Similarly, ALDHTt displayed activity for two different esterase substrates, p-nitrophenyl acetate and p-nitrophenyl butyrate, but with activities of 22.9% and 8.9%, respectively, compared to the activity towards hexanal." @default.
- W4200529156 created "2021-12-31" @default.
- W4200529156 creator A5018893421 @default.
- W4200529156 creator A5049955442 @default.
- W4200529156 creator A5074610031 @default.
- W4200529156 creator A5087699949 @default.
- W4200529156 date "2021-12-14" @default.
- W4200529156 modified "2023-09-30" @default.
- W4200529156 title "Study of ALDH from Thermus thermophilus—Expression, Purification and Characterisation of the Non-Substrate Specific, Thermophilic Enzyme Displaying Both Dehydrogenase and Esterase Activity" @default.
- W4200529156 cites W1565629087 @default.
- W4200529156 cites W1579417853 @default.
- W4200529156 cites W1604995875 @default.
- W4200529156 cites W1859443916 @default.
- W4200529156 cites W1904617196 @default.
- W4200529156 cites W1955249161 @default.
- W4200529156 cites W1963889064 @default.
- W4200529156 cites W1964401828 @default.
- W4200529156 cites W1966808628 @default.
- W4200529156 cites W1968105649 @default.
- W4200529156 cites W1969139245 @default.
- W4200529156 cites W1970150587 @default.
- W4200529156 cites W1971703766 @default.
- W4200529156 cites W1977444582 @default.
- W4200529156 cites W1978290357 @default.
- W4200529156 cites W1980199874 @default.
- W4200529156 cites W1982711007 @default.
- W4200529156 cites W1985164910 @default.
- W4200529156 cites W1990769556 @default.
- W4200529156 cites W1991691234 @default.
- W4200529156 cites W2003609346 @default.
- W4200529156 cites W2008237642 @default.
- W4200529156 cites W2008904725 @default.
- W4200529156 cites W2012719891 @default.
- W4200529156 cites W2020821598 @default.
- W4200529156 cites W2025650487 @default.
- W4200529156 cites W2028753394 @default.
- W4200529156 cites W2032136089 @default.
- W4200529156 cites W2032202673 @default.
- W4200529156 cites W2034110157 @default.
- W4200529156 cites W2038468745 @default.
- W4200529156 cites W2044212453 @default.
- W4200529156 cites W2047626540 @default.
- W4200529156 cites W2056215545 @default.
- W4200529156 cites W2061813962 @default.
- W4200529156 cites W2073656127 @default.
- W4200529156 cites W2077657779 @default.
- W4200529156 cites W2078461196 @default.
- W4200529156 cites W2081351575 @default.
- W4200529156 cites W2083042164 @default.
- W4200529156 cites W2094878571 @default.
- W4200529156 cites W2114892178 @default.
- W4200529156 cites W2115871167 @default.
- W4200529156 cites W2122742102 @default.
- W4200529156 cites W2143720549 @default.
- W4200529156 cites W2144190812 @default.
- W4200529156 cites W2151238119 @default.
- W4200529156 cites W2156769423 @default.
- W4200529156 cites W2156895606 @default.
- W4200529156 cites W2157851561 @default.
- W4200529156 cites W2298207820 @default.
- W4200529156 cites W2432093418 @default.
- W4200529156 cites W2515021511 @default.
- W4200529156 cites W2533419568 @default.
- W4200529156 cites W2619467199 @default.
- W4200529156 cites W2858783432 @default.
- W4200529156 cites W2889063371 @default.
- W4200529156 cites W2890629558 @default.
- W4200529156 cites W2905135446 @default.
- W4200529156 cites W2909429444 @default.
- W4200529156 cites W2910094671 @default.
- W4200529156 cites W2914434672 @default.
- W4200529156 cites W2920974307 @default.
- W4200529156 cites W2999967898 @default.
- W4200529156 cites W3004065784 @default.
- W4200529156 cites W3032881716 @default.
- W4200529156 cites W3040929635 @default.
- W4200529156 cites W3044918266 @default.
- W4200529156 cites W3045825584 @default.
- W4200529156 cites W3088964070 @default.
- W4200529156 cites W3093910874 @default.
- W4200529156 cites W3119126409 @default.
- W4200529156 cites W3132515687 @default.
- W4200529156 cites W3163560222 @default.
- W4200529156 cites W4256113555 @default.
- W4200529156 cites W4720463 @default.
- W4200529156 cites W934960873 @default.
- W4200529156 cites W1987974614 @default.
- W4200529156 doi "https://doi.org/10.3390/cells10123535" @default.
- W4200529156 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34944041" @default.
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