Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200533304> ?p ?o ?g. }
- W4200533304 endingPage "230" @default.
- W4200533304 startingPage "230" @default.
- W4200533304 abstract "DNA polymerase η (Polη) is a translesion synthesis polymerase that can bypass different DNA lesions with varying efficiency and fidelity. Its most well-known function is the error-free bypass of ultraviolet light-induced cyclobutane pyrimidine dimers. The lack of this unique ability in humans leads to the development of a cancer-predisposing disease, the variant form of xeroderma pigmentosum. Human Polη can insert rNTPs during DNA synthesis, though with much lower efficiency than dNTPs, and it can even extend an RNA chain with ribonucleotides. We have previously shown that Mn2+ is a specific activator of the RNA synthetic activity of yeast Polη that increases the efficiency of the reaction by several thousand-fold over Mg2+. In this study, our goal was to investigate the metal cofactor dependence of RNA synthesis by human Polη. We found that out of the investigated metal cations, only Mn2+ supported robust RNA synthesis. Steady state kinetic analysis showed that Mn2+ activated the reaction a thousand-fold compared to Mg2+, even during DNA damage bypass opposite 8-oxoG and TT dimer. Our results revealed a two order of magnitude higher affinity of human Polη towards ribonucleotides in the presence of Mn2+ compared to Mg2+. It is noteworthy that activation occurred without lowering the base selectivity of the enzyme on undamaged templates, whereas the fidelity decreased across a TT dimer. In summary, our data strongly suggest that, like with its yeast homolog, Mn2+ is the proper metal cofactor of hPolη during RNA chain extension, and selective metal cofactor utilization contributes to switching between its DNA and RNA synthetic activities." @default.
- W4200533304 created "2021-12-31" @default.
- W4200533304 creator A5016498563 @default.
- W4200533304 creator A5031981833 @default.
- W4200533304 date "2021-12-26" @default.
- W4200533304 modified "2023-10-06" @default.
- W4200533304 title "Manganese Is a Strong Specific Activator of the RNA Synthetic Activity of Human Polη" @default.
- W4200533304 cites W1522788044 @default.
- W4200533304 cites W1531823089 @default.
- W4200533304 cites W1970184264 @default.
- W4200533304 cites W2000774068 @default.
- W4200533304 cites W2004648009 @default.
- W4200533304 cites W2022265644 @default.
- W4200533304 cites W2023871617 @default.
- W4200533304 cites W2037845691 @default.
- W4200533304 cites W2059227077 @default.
- W4200533304 cites W2064639767 @default.
- W4200533304 cites W2065286203 @default.
- W4200533304 cites W2074050403 @default.
- W4200533304 cites W2077036683 @default.
- W4200533304 cites W2084616101 @default.
- W4200533304 cites W2098411964 @default.
- W4200533304 cites W2113356069 @default.
- W4200533304 cites W2142233919 @default.
- W4200533304 cites W2143064529 @default.
- W4200533304 cites W2146230109 @default.
- W4200533304 cites W2146289791 @default.
- W4200533304 cites W2149355104 @default.
- W4200533304 cites W2164402320 @default.
- W4200533304 cites W2172105250 @default.
- W4200533304 cites W2237287274 @default.
- W4200533304 cites W2323867229 @default.
- W4200533304 cites W2563101450 @default.
- W4200533304 cites W2594378117 @default.
- W4200533304 cites W2760828789 @default.
- W4200533304 cites W2762403097 @default.
- W4200533304 cites W2811434952 @default.
- W4200533304 cites W2912435840 @default.
- W4200533304 cites W2921362401 @default.
- W4200533304 cites W3095191815 @default.
- W4200533304 cites W3119311457 @default.
- W4200533304 cites W3200117166 @default.
- W4200533304 doi "https://doi.org/10.3390/ijms23010230" @default.
- W4200533304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35008656" @default.
- W4200533304 hasPublicationYear "2021" @default.
- W4200533304 type Work @default.
- W4200533304 citedByCount "1" @default.
- W4200533304 countsByYear W42005333042023 @default.
- W4200533304 crossrefType "journal-article" @default.
- W4200533304 hasAuthorship W4200533304A5016498563 @default.
- W4200533304 hasAuthorship W4200533304A5031981833 @default.
- W4200533304 hasBestOaLocation W42005333041 @default.
- W4200533304 hasConcept C104317684 @default.
- W4200533304 hasConcept C12554922 @default.
- W4200533304 hasConcept C143425029 @default.
- W4200533304 hasConcept C153911025 @default.
- W4200533304 hasConcept C167975912 @default.
- W4200533304 hasConcept C181199279 @default.
- W4200533304 hasConcept C185592680 @default.
- W4200533304 hasConcept C197957613 @default.
- W4200533304 hasConcept C552990157 @default.
- W4200533304 hasConcept C55493867 @default.
- W4200533304 hasConcept C67705224 @default.
- W4200533304 hasConcept C82381507 @default.
- W4200533304 hasConcept C86803240 @default.
- W4200533304 hasConceptScore W4200533304C104317684 @default.
- W4200533304 hasConceptScore W4200533304C12554922 @default.
- W4200533304 hasConceptScore W4200533304C143425029 @default.
- W4200533304 hasConceptScore W4200533304C153911025 @default.
- W4200533304 hasConceptScore W4200533304C167975912 @default.
- W4200533304 hasConceptScore W4200533304C181199279 @default.
- W4200533304 hasConceptScore W4200533304C185592680 @default.
- W4200533304 hasConceptScore W4200533304C197957613 @default.
- W4200533304 hasConceptScore W4200533304C552990157 @default.
- W4200533304 hasConceptScore W4200533304C55493867 @default.
- W4200533304 hasConceptScore W4200533304C67705224 @default.
- W4200533304 hasConceptScore W4200533304C82381507 @default.
- W4200533304 hasConceptScore W4200533304C86803240 @default.
- W4200533304 hasIssue "1" @default.
- W4200533304 hasLocation W42005333041 @default.
- W4200533304 hasLocation W42005333042 @default.
- W4200533304 hasLocation W42005333043 @default.
- W4200533304 hasLocation W42005333044 @default.
- W4200533304 hasLocation W42005333045 @default.
- W4200533304 hasOpenAccess W4200533304 @default.
- W4200533304 hasPrimaryLocation W42005333041 @default.
- W4200533304 hasRelatedWork W145051234 @default.
- W4200533304 hasRelatedWork W1976736675 @default.
- W4200533304 hasRelatedWork W1998007030 @default.
- W4200533304 hasRelatedWork W2002473487 @default.
- W4200533304 hasRelatedWork W2036669013 @default.
- W4200533304 hasRelatedWork W2045993401 @default.
- W4200533304 hasRelatedWork W2048925025 @default.
- W4200533304 hasRelatedWork W2052228805 @default.
- W4200533304 hasRelatedWork W2143246881 @default.
- W4200533304 hasRelatedWork W1966182317 @default.
- W4200533304 hasVolume "23" @default.
- W4200533304 isParatext "false" @default.