Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200556727> ?p ?o ?g. }
- W4200556727 abstract "Abstract Microtubules are major cytoskeletal filaments that drive chromosome separation during cell division, serve as rails for intracellular transport and as a scaffold for organelle positioning. Experimental manipulation of microtubules is widely used in cell and developmental biology, but tools for precise subcellular spatiotemporal control of microtubule integrity are currently lacking. Here, we exploit the dependence of the mammalian microtubule-severing protein katanin on microtubule-targeting co-factors to generate a light-activated system for localized microtubule disassembly that we named opto-katanin. Targeted illumination with blue light induces rapid and localized opto-katanin recruitment and local microtubule depolymerization, which is quickly reversible after stopping light-induced activation. Opto-katanin can be employed to locally perturb microtubule-based transport and organelle morphology in dividing cells and differentiated neurons with high spatiotemporal precision. We show that different microtubule-associated proteins can be used to recruit opto-katanin to microtubules and induce severing, paving the way for spatiotemporally precise manipulation of specific microtubule subpopulations." @default.
- W4200556727 created "2021-12-31" @default.
- W4200556727 creator A5002852262 @default.
- W4200556727 creator A5019567999 @default.
- W4200556727 creator A5025164063 @default.
- W4200556727 creator A5052178429 @default.
- W4200556727 creator A5087012385 @default.
- W4200556727 date "2021-12-23" @default.
- W4200556727 modified "2023-10-16" @default.
- W4200556727 title "Opto-Katanin: An Optogenetic Tool for Localized Microtubule Disassembly" @default.
- W4200556727 cites W1526729571 @default.
- W4200556727 cites W1755456091 @default.
- W4200556727 cites W1982409013 @default.
- W4200556727 cites W1985084749 @default.
- W4200556727 cites W1988840491 @default.
- W4200556727 cites W2034442307 @default.
- W4200556727 cites W2036008741 @default.
- W4200556727 cites W2039025686 @default.
- W4200556727 cites W2044820159 @default.
- W4200556727 cites W2047730219 @default.
- W4200556727 cites W2057660228 @default.
- W4200556727 cites W2063408699 @default.
- W4200556727 cites W2066486174 @default.
- W4200556727 cites W2071202520 @default.
- W4200556727 cites W2077214620 @default.
- W4200556727 cites W2084575956 @default.
- W4200556727 cites W2092782214 @default.
- W4200556727 cites W2095674737 @default.
- W4200556727 cites W2096938747 @default.
- W4200556727 cites W2099220194 @default.
- W4200556727 cites W2113144687 @default.
- W4200556727 cites W2117121945 @default.
- W4200556727 cites W2127901427 @default.
- W4200556727 cites W2136880905 @default.
- W4200556727 cites W2143764910 @default.
- W4200556727 cites W2144167875 @default.
- W4200556727 cites W2145033500 @default.
- W4200556727 cites W2152331121 @default.
- W4200556727 cites W2239857015 @default.
- W4200556727 cites W2313059359 @default.
- W4200556727 cites W2336101489 @default.
- W4200556727 cites W2336853363 @default.
- W4200556727 cites W2460798922 @default.
- W4200556727 cites W2511440169 @default.
- W4200556727 cites W2523225100 @default.
- W4200556727 cites W2560056367 @default.
- W4200556727 cites W2608056363 @default.
- W4200556727 cites W2766776262 @default.
- W4200556727 cites W2784080846 @default.
- W4200556727 cites W2792066084 @default.
- W4200556727 cites W2888209032 @default.
- W4200556727 cites W2892048355 @default.
- W4200556727 cites W2913674846 @default.
- W4200556727 cites W2962851207 @default.
- W4200556727 cites W2972266688 @default.
- W4200556727 cites W2982926407 @default.
- W4200556727 cites W3000358775 @default.
- W4200556727 cites W3005654419 @default.
- W4200556727 cites W3006603801 @default.
- W4200556727 cites W3006804533 @default.
- W4200556727 cites W3083369693 @default.
- W4200556727 cites W3087649796 @default.
- W4200556727 cites W3093807067 @default.
- W4200556727 cites W3102863060 @default.
- W4200556727 cites W3109677595 @default.
- W4200556727 cites W3135111030 @default.
- W4200556727 cites W3183692149 @default.
- W4200556727 cites W3194916623 @default.
- W4200556727 cites W3205049334 @default.
- W4200556727 cites W3213001096 @default.
- W4200556727 doi "https://doi.org/10.1101/2021.12.22.473806" @default.
- W4200556727 hasPublicationYear "2021" @default.
- W4200556727 type Work @default.
- W4200556727 citedByCount "1" @default.
- W4200556727 countsByYear W42005567272022 @default.
- W4200556727 crossrefType "posted-content" @default.
- W4200556727 hasAuthorship W4200556727A5002852262 @default.
- W4200556727 hasAuthorship W4200556727A5019567999 @default.
- W4200556727 hasAuthorship W4200556727A5025164063 @default.
- W4200556727 hasAuthorship W4200556727A5052178429 @default.
- W4200556727 hasAuthorship W4200556727A5087012385 @default.
- W4200556727 hasBestOaLocation W42005567271 @default.
- W4200556727 hasConcept C10447061 @default.
- W4200556727 hasConcept C142669718 @default.
- W4200556727 hasConcept C1491633281 @default.
- W4200556727 hasConcept C168240541 @default.
- W4200556727 hasConcept C20418707 @default.
- W4200556727 hasConcept C29537977 @default.
- W4200556727 hasConcept C55493867 @default.
- W4200556727 hasConcept C61081601 @default.
- W4200556727 hasConcept C86803240 @default.
- W4200556727 hasConcept C94921385 @default.
- W4200556727 hasConcept C95444343 @default.
- W4200556727 hasConceptScore W4200556727C10447061 @default.
- W4200556727 hasConceptScore W4200556727C142669718 @default.
- W4200556727 hasConceptScore W4200556727C1491633281 @default.
- W4200556727 hasConceptScore W4200556727C168240541 @default.
- W4200556727 hasConceptScore W4200556727C20418707 @default.
- W4200556727 hasConceptScore W4200556727C29537977 @default.
- W4200556727 hasConceptScore W4200556727C55493867 @default.