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• W4200575478 endingPage "13597" @default.
• W4200575478 startingPage "13597" @default.
• W4200575478 abstract "The human T-cell leukemia virus type 1 (HTLV-1)-encoded transactivator and oncoprotein Tax-1 is essential for HTLV-1 replication. We recently found that Tax-1 interacts with transcription elongation factor for RNA polymerase II 2, ELL2, which enhances Tax-1-mediated transactivation of the HTLV-1 promotor. Here, we characterize the Tax-1:ELL2 interaction and its impact on viral transactivation by confocal imaging, co-immunoprecipitation, and luciferase assays. We found that Tax-1 and ELL2 not only co-precipitate, but also co-localize in dot-like structures in the nucleus. Tax-1:ELL2 complex formation occurred independently of Tax-1 point mutations, which are crucial for post translational modifications (PTMs) of Tax-1, suggesting that these PTMs are irrelevant for Tax-1:ELL2 interaction. In contrast, Tax-1 deletion mutants lacking either N-terminal (aa 1-37) or C-terminal regions (aa 150-353) of Tax-1 were impaired in interacting with ELL2. Contrary to Tax-1, the related, non-oncogenic Tax-2B from HTLV-2B did not interact with ELL2. Finally, we found that ELL2-R1 (aa 1-353), which carries an RNA polymerase II binding domain, and ELL2-R3 (aa 515-640) are sufficient to interact with Tax-1; however, only ELL2-truncations expressing R1 could enhance Tax-1-mediated transactivation of the HTLV-1 promoter. Together, this study identifies domains in Tax-1 and ELL2 being required for Tax-1:ELL2 complex formation and for viral transactivation." @default.
• W4200575478 created "2021-12-31" @default.
• W4200575478 creator A5004357156 @default.
• W4200575478 creator A5065393453 @default.
• W4200575478 creator A5068913705 @default.
• W4200575478 creator A5069109467 @default.
• W4200575478 creator A5088718255 @default.
• W4200575478 creator A5088784630 @default.
• W4200575478 date "2021-12-18" @default.
• W4200575478 modified "2023-10-17" @default.
• W4200575478 title "Characterizing the Interaction between the HTLV-1 Transactivator Tax-1 with Transcription Elongation Factor ELL2 and Its Impact on Viral Transactivation" @default.
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• W4200575478 doi "https://doi.org/10.3390/ijms222413597" @default.
• W4200575478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34948391" @default.

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