FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200575596> ?p ?o ?g. }

• W4200575596 abstract "Oral squamous cell carcinoma (OSCC) has poor survival rates. There is a pressing need to develop more precise risk assessment methods to tailor clinical treatment. Epigenome-wide association studies in OSCC have not produced a viable biomarker. These studies have relied on methylation array platforms, which are limited in their ability to profile the methylome. In this study, we use MethylCap-Seq (MC-Seq), a comprehensive methylation quantification technique, and brush swab samples, to develop a noninvasive, readily translatable approach to profile the methylome in OSCC patients.Three OSCC patients underwent collection of cancer and contralateral normal tissue and brush swab biopsies, totaling 4 samples for each patient. Epigenome-wide DNA methylation quantification was performed using the SureSelectXT Methyl-Seq platform. DNA quality and methylation site resolution were compared between brush swab and tissue samples. Correlation and methylation value difference were determined for brush swabs vs. tissues for each respective patient and site (i.e., cancer or normal). Correlations were calculated between cancer and normal tissues and brush swab samples for each patient to determine the robustness of DNA methylation marks using brush swabs in clinical biomarker studies.There were no significant differences in DNA yield between tissue and brush swab samples. Mapping efficiency exceeded 90% across all samples, with no differences between tissue and brush swabs. The average number of CpG sites with at least 10x depth of coverage was 2,716,674 for brush swabs and 2,903,261 for tissues. Matched tissue and brush swabs had excellent correlation (r = 0.913 for cancer samples and r = 0.951 for normal samples). The methylation profile of the top 1000 CpGs was significantly different between cancer and normal samples (mean p-value = 0.00021) but not different between tissues and brush swabs (mean p-value = 0.11).Our results demonstrate that MC-Seq is an efficient platform for epigenome profiling in cancer biomarker studies, with broader methylome coverage than array-based platforms. Brush swab biopsy provides adequate DNA yield for MC-Seq, and taken together, our findings set the stage for development of a non-invasive methylome quantification technique for oral cancer with high translational potential." @default.
• W4200575596 created "2021-12-31" @default.
• W4200575596 creator A5000178954 @default.
• W4200575596 creator A5008145165 @default.
• W4200575596 creator A5011972670 @default.
• W4200575596 creator A5017674134 @default.
• W4200575596 creator A5021288344 @default.
• W4200575596 creator A5021429069 @default.
• W4200575596 creator A5028516814 @default.
• W4200575596 creator A5030395315 @default.
• W4200575596 creator A5031651994 @default.
• W4200575596 creator A5036741538 @default.
• W4200575596 creator A5039308697 @default.
• W4200575596 creator A5046173951 @default.
• W4200575596 creator A5055309934 @default.
• W4200575596 creator A5055492168 @default.
• W4200575596 creator A5065071537 @default.
• W4200575596 creator A5072286188 @default.
• W4200575596 creator A5072560123 @default.
• W4200575596 creator A5080341418 @default.
• W4200575596 creator A5082312520 @default.
• W4200575596 creator A5082834975 @default.
• W4200575596 creator A5086420028 @default.
• W4200575596 creator A5086627090 @default.
• W4200575596 creator A5090838237 @default.
• W4200575596 date "2021-12-01" @default.
• W4200575596 modified "2023-10-14" @default.
• W4200575596 title "Brush swab as a noninvasive surrogate for tissue biopsies in epigenomic profiling of oral cancer" @default.
• W4200575596 cites W112159788 @default.
• W4200575596 cites W1592123008 @default.
• W4200575596 cites W1972068299 @default.
• W4200575596 cites W1983102362 @default.
• W4200575596 cites W2012678233 @default.
• W4200575596 cites W2063638796 @default.
• W4200575596 cites W2111406963 @default.
• W4200575596 cites W2118150538 @default.
• W4200575596 cites W2137847072 @default.
• W4200575596 cites W2138804607 @default.
• W4200575596 cites W2154195517 @default.
• W4200575596 cites W2304496028 @default.
• W4200575596 cites W2427267007 @default.
• W4200575596 cites W2555498571 @default.
• W4200575596 cites W2579941249 @default.
• W4200575596 cites W2593073653 @default.
• W4200575596 cites W2750126117 @default.
• W4200575596 cites W2769970855 @default.
• W4200575596 cites W2792893163 @default.
• W4200575596 cites W2809466659 @default.
• W4200575596 cites W2971956569 @default.
• W4200575596 cites W3001917418 @default.
• W4200575596 cites W3024593376 @default.
• W4200575596 cites W3084773198 @default.
• W4200575596 cites W3107798130 @default.
• W4200575596 cites W3122075075 @default.
• W4200575596 cites W3168981837 @default.
• W4200575596 doi "https://doi.org/10.1186/s40364-021-00349-x" @default.
• W4200575596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34930473" @default.
• W4200575596 hasPublicationYear "2021" @default.
• W4200575596 type Work @default.
• W4200575596 citedByCount "5" @default.
• W4200575596 countsByYear W42005755962022 @default.
• W4200575596 countsByYear W42005755962023 @default.
• W4200575596 crossrefType "journal-article" @default.
• W4200575596 hasAuthorship W4200575596A5000178954 @default.
• W4200575596 hasAuthorship W4200575596A5008145165 @default.
• W4200575596 hasAuthorship W4200575596A5011972670 @default.
• W4200575596 hasAuthorship W4200575596A5017674134 @default.
• W4200575596 hasAuthorship W4200575596A5021288344 @default.
• W4200575596 hasAuthorship W4200575596A5021429069 @default.
• W4200575596 hasAuthorship W4200575596A5028516814 @default.
• W4200575596 hasAuthorship W4200575596A5030395315 @default.
• W4200575596 hasAuthorship W4200575596A5031651994 @default.
• W4200575596 hasAuthorship W4200575596A5036741538 @default.
• W4200575596 hasAuthorship W4200575596A5039308697 @default.
• W4200575596 hasAuthorship W4200575596A5046173951 @default.
• W4200575596 hasAuthorship W4200575596A5055309934 @default.
• W4200575596 hasAuthorship W4200575596A5055492168 @default.
• W4200575596 hasAuthorship W4200575596A5065071537 @default.
• W4200575596 hasAuthorship W4200575596A5072286188 @default.
• W4200575596 hasAuthorship W4200575596A5072560123 @default.
• W4200575596 hasAuthorship W4200575596A5080341418 @default.
• W4200575596 hasAuthorship W4200575596A5082312520 @default.
• W4200575596 hasAuthorship W4200575596A5082834975 @default.
• W4200575596 hasAuthorship W4200575596A5086420028 @default.
• W4200575596 hasAuthorship W4200575596A5086627090 @default.
• W4200575596 hasAuthorship W4200575596A5090838237 @default.
• W4200575596 hasBestOaLocation W42005755961 @default.
• W4200575596 hasConcept C104317684 @default.
• W4200575596 hasConcept C117717151 @default.
• W4200575596 hasConcept C119599485 @default.
• W4200575596 hasConcept C121608353 @default.
• W4200575596 hasConcept C121912465 @default.
• W4200575596 hasConcept C126322002 @default.
• W4200575596 hasConcept C127413603 @default.
• W4200575596 hasConcept C142724271 @default.
• W4200575596 hasConcept C150194340 @default.
• W4200575596 hasConcept C16108499 @default.
• W4200575596 hasConcept C190727270 @default.
• W4200575596 hasConcept C2775934546 @default.
• W4200575596 hasConcept C2781197716 @default.

• next