Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200602646> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W4200602646 endingPage "106395" @default.
- W4200602646 startingPage "106395" @default.
- W4200602646 abstract "Enterotoxaemia, a disease that affects domestic ruminants, is caused by the epsilon toxin of Clostridium perfringens type D and B. Control and prophylaxis are based on systemic vaccination of small ruminant herds with epsilon toxoid. Purified epsilon toxin is an essential material for vaccine evaluation. It is also necessary for diagnosis of enterotoxaemia disease in the field by in vitro tests including ELISA. The aim of this study was to set up a method for preparation of functional purified epsilon toxin of C. perfringens type D to be used in serum neutralization test. In this study, epsilon toxin was prepared from C. perfringens type D culture precipitated with ammonium sulfate, dialyzed against phosphate buffered saline (PBS) buffer and then, purified using chromatography system. Then, the purified epsilon toxin was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Toxin function was confirmed by cell culture and minimum lethal dose (MLD) assays. Also rabbits were immunized by vaccine in two turns with a 28-day interval. Then, blood samples were collected, and serum neutralization (SN) test was carried out. Results showed that the purified toxin was suitable for SN assay. Our purification method was simple, fast and cost-effective for preparation of epsilon toxin." @default.
- W4200602646 created "2021-12-31" @default.
- W4200602646 creator A5024744870 @default.
- W4200602646 creator A5038727242 @default.
- W4200602646 creator A5043339311 @default.
- W4200602646 date "2022-02-01" @default.
- W4200602646 modified "2023-10-17" @default.
- W4200602646 title "A simple method for purification of epsilon toxin of Clostridium perfringens type D for serum neutralization assay" @default.
- W4200602646 cites W1483281977 @default.
- W4200602646 cites W1775749144 @default.
- W4200602646 cites W1847045814 @default.
- W4200602646 cites W1943005411 @default.
- W4200602646 cites W1966689948 @default.
- W4200602646 cites W1968042696 @default.
- W4200602646 cites W1969209049 @default.
- W4200602646 cites W2032896244 @default.
- W4200602646 cites W2049872880 @default.
- W4200602646 cites W2061142159 @default.
- W4200602646 cites W2061481381 @default.
- W4200602646 cites W2084992616 @default.
- W4200602646 cites W2094413622 @default.
- W4200602646 cites W2097937789 @default.
- W4200602646 cites W2099792326 @default.
- W4200602646 cites W2102792821 @default.
- W4200602646 cites W2126451395 @default.
- W4200602646 cites W2126573195 @default.
- W4200602646 cites W2142095828 @default.
- W4200602646 cites W2162844206 @default.
- W4200602646 cites W2256756668 @default.
- W4200602646 cites W2426516991 @default.
- W4200602646 cites W3133912702 @default.
- W4200602646 cites W3155392107 @default.
- W4200602646 cites W956392526 @default.
- W4200602646 doi "https://doi.org/10.1016/j.mimet.2021.106395" @default.
- W4200602646 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34921869" @default.
- W4200602646 hasPublicationYear "2022" @default.
- W4200602646 type Work @default.
- W4200602646 citedByCount "2" @default.
- W4200602646 countsByYear W42006026462022 @default.
- W4200602646 countsByYear W42006026462023 @default.
- W4200602646 crossrefType "journal-article" @default.
- W4200602646 hasAuthorship W4200602646A5024744870 @default.
- W4200602646 hasAuthorship W4200602646A5038727242 @default.
- W4200602646 hasAuthorship W4200602646A5043339311 @default.
- W4200602646 hasConcept C116084860 @default.
- W4200602646 hasConcept C14086860 @default.
- W4200602646 hasConcept C153911025 @default.
- W4200602646 hasConcept C159047783 @default.
- W4200602646 hasConcept C185592680 @default.
- W4200602646 hasConcept C22070199 @default.
- W4200602646 hasConcept C2522874641 @default.
- W4200602646 hasConcept C2777351567 @default.
- W4200602646 hasConcept C2777367657 @default.
- W4200602646 hasConcept C2778913249 @default.
- W4200602646 hasConcept C2779116991 @default.
- W4200602646 hasConcept C2779841045 @default.
- W4200602646 hasConcept C43617362 @default.
- W4200602646 hasConcept C523546767 @default.
- W4200602646 hasConcept C54355233 @default.
- W4200602646 hasConcept C86803240 @default.
- W4200602646 hasConcept C89423630 @default.
- W4200602646 hasConceptScore W4200602646C116084860 @default.
- W4200602646 hasConceptScore W4200602646C14086860 @default.
- W4200602646 hasConceptScore W4200602646C153911025 @default.
- W4200602646 hasConceptScore W4200602646C159047783 @default.
- W4200602646 hasConceptScore W4200602646C185592680 @default.
- W4200602646 hasConceptScore W4200602646C22070199 @default.
- W4200602646 hasConceptScore W4200602646C2522874641 @default.
- W4200602646 hasConceptScore W4200602646C2777351567 @default.
- W4200602646 hasConceptScore W4200602646C2777367657 @default.
- W4200602646 hasConceptScore W4200602646C2778913249 @default.
- W4200602646 hasConceptScore W4200602646C2779116991 @default.
- W4200602646 hasConceptScore W4200602646C2779841045 @default.
- W4200602646 hasConceptScore W4200602646C43617362 @default.
- W4200602646 hasConceptScore W4200602646C523546767 @default.
- W4200602646 hasConceptScore W4200602646C54355233 @default.
- W4200602646 hasConceptScore W4200602646C86803240 @default.
- W4200602646 hasConceptScore W4200602646C89423630 @default.
- W4200602646 hasLocation W42006026461 @default.
- W4200602646 hasLocation W42006026462 @default.
- W4200602646 hasOpenAccess W4200602646 @default.
- W4200602646 hasPrimaryLocation W42006026461 @default.
- W4200602646 hasRelatedWork W114416092 @default.
- W4200602646 hasRelatedWork W1575120037 @default.
- W4200602646 hasRelatedWork W18255195 @default.
- W4200602646 hasRelatedWork W1970721807 @default.
- W4200602646 hasRelatedWork W2003815947 @default.
- W4200602646 hasRelatedWork W2095565040 @default.
- W4200602646 hasRelatedWork W2411817821 @default.
- W4200602646 hasRelatedWork W2468926082 @default.
- W4200602646 hasRelatedWork W2890318181 @default.
- W4200602646 hasRelatedWork W4200602646 @default.
- W4200602646 hasVolume "193" @default.
- W4200602646 isParatext "false" @default.
- W4200602646 isRetracted "false" @default.
- W4200602646 workType "article" @default.