Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200608399> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W4200608399 abstract "Urinary tract infection (UTI) is an infection caused by the presence and growth of microorganisms anywhere in the urinary tract. It is usually due to bacteria from the digestive tract which climb the opening of the urethra and begin to multiply to cause infection. However, UTI is more frequent in female than male, because of the short urethra, absence of prostatic secretion, and pregnancy.This study was aimed to detect extended spectrum beta lactamase and MCR-1genes from Gram negative bacterial clinical isolates from urinary tract infections. Ninety-one urine samples were collected in this study, then cultured on CLED agar and identified by conventional biochemical methods. Modified Kirby-Bauer method was used for sensitivity testing. Genomic DNA extracted by boiling method, and multiplex PCR was conducted to amplify TEM, AmpC, MCR-1, SHV, and CTX-M genes from all Gram-negative isolates.The result of the susceptibility test revealed that the highest resistant rates were 73% for Ceftazidime, followed by 63%, 56%, and 55% for Ciprofloxacin, Gentamicin, and Co-trimoxazole, respectively, and 21% were resistant to Imipenem. Moreover, for the presence of resistance genes, multiplex PCR results displayed that the TEM gene was present in 34% of bacteria, AmpC gene was found in 49.4% of isolates. Also, 38.5% and 6.6% were positive for MCR-1 gene and SHV gene, respectively. All Proteus species were negative to MCR-1 and TEM genes. Fifty E. coli, 7 Klebsiella pneumonia, two Pseudomonas aeruginosa, and five Proteus species were positive for CTX-M gene and all Citrobacter spp. were negative for CTX-M gene. Eighty-nine isolates were positive for one or more ESBL genes, while two isolates were negative to all genes. CTX-M gene is predominant among uropathogenic bacteria and imipenem is the best effective antibiotic.This recent study proved that the result of the susceptibility test revealed that the highest resistant rate were 73% for Ceftazidime, followed by 63%, 56%, and 55% for Ciprofloxacin, Gentamicin, and Co-trimoxazole, respectively, and 21% were resistant to imipenem." @default.
- W4200608399 created "2021-12-31" @default.
- W4200608399 creator A5000960109 @default.
- W4200608399 creator A5026967550 @default.
- W4200608399 creator A5030976538 @default.
- W4200608399 creator A5051642655 @default.
- W4200608399 creator A5069623347 @default.
- W4200608399 creator A5070218533 @default.
- W4200608399 creator A5077551177 @default.
- W4200608399 creator A5088258470 @default.
- W4200608399 date "2021-01-01" @default.
- W4200608399 modified "2023-09-26" @default.
- W4200608399 title "Detection of TEM, AmpC, SHV, CTX-M, and MCR-1 Genes in Gram Negative Isolates of Urinary Tract Infections" @default.
- W4200608399 doi "https://doi.org/10.7754/clin.lab.2021.210315" @default.
- W4200608399 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34910434" @default.
- W4200608399 hasPublicationYear "2021" @default.
- W4200608399 type Work @default.
- W4200608399 citedByCount "1" @default.
- W4200608399 countsByYear W42006083992023 @default.
- W4200608399 crossrefType "journal-article" @default.
- W4200608399 hasAuthorship W4200608399A5000960109 @default.
- W4200608399 hasAuthorship W4200608399A5026967550 @default.
- W4200608399 hasAuthorship W4200608399A5030976538 @default.
- W4200608399 hasAuthorship W4200608399A5051642655 @default.
- W4200608399 hasAuthorship W4200608399A5069623347 @default.
- W4200608399 hasAuthorship W4200608399A5070218533 @default.
- W4200608399 hasAuthorship W4200608399A5077551177 @default.
- W4200608399 hasAuthorship W4200608399A5088258470 @default.
- W4200608399 hasConcept C104317684 @default.
- W4200608399 hasConcept C134018914 @default.
- W4200608399 hasConcept C2775832221 @default.
- W4200608399 hasConcept C2776933286 @default.
- W4200608399 hasConcept C2776968632 @default.
- W4200608399 hasConcept C2777058267 @default.
- W4200608399 hasConcept C2777637488 @default.
- W4200608399 hasConcept C2778512257 @default.
- W4200608399 hasConcept C2779464495 @default.
- W4200608399 hasConcept C2779631663 @default.
- W4200608399 hasConcept C2780026642 @default.
- W4200608399 hasConcept C49105822 @default.
- W4200608399 hasConcept C501593827 @default.
- W4200608399 hasConcept C523546767 @default.
- W4200608399 hasConcept C54355233 @default.
- W4200608399 hasConcept C547475151 @default.
- W4200608399 hasConcept C55493867 @default.
- W4200608399 hasConcept C77411442 @default.
- W4200608399 hasConcept C86803240 @default.
- W4200608399 hasConcept C89423630 @default.
- W4200608399 hasConcept C90583042 @default.
- W4200608399 hasConcept C94665300 @default.
- W4200608399 hasConceptScore W4200608399C104317684 @default.
- W4200608399 hasConceptScore W4200608399C134018914 @default.
- W4200608399 hasConceptScore W4200608399C2775832221 @default.
- W4200608399 hasConceptScore W4200608399C2776933286 @default.
- W4200608399 hasConceptScore W4200608399C2776968632 @default.
- W4200608399 hasConceptScore W4200608399C2777058267 @default.
- W4200608399 hasConceptScore W4200608399C2777637488 @default.
- W4200608399 hasConceptScore W4200608399C2778512257 @default.
- W4200608399 hasConceptScore W4200608399C2779464495 @default.
- W4200608399 hasConceptScore W4200608399C2779631663 @default.
- W4200608399 hasConceptScore W4200608399C2780026642 @default.
- W4200608399 hasConceptScore W4200608399C49105822 @default.
- W4200608399 hasConceptScore W4200608399C501593827 @default.
- W4200608399 hasConceptScore W4200608399C523546767 @default.
- W4200608399 hasConceptScore W4200608399C54355233 @default.
- W4200608399 hasConceptScore W4200608399C547475151 @default.
- W4200608399 hasConceptScore W4200608399C55493867 @default.
- W4200608399 hasConceptScore W4200608399C77411442 @default.
- W4200608399 hasConceptScore W4200608399C86803240 @default.
- W4200608399 hasConceptScore W4200608399C89423630 @default.
- W4200608399 hasConceptScore W4200608399C90583042 @default.
- W4200608399 hasConceptScore W4200608399C94665300 @default.
- W4200608399 hasIssue "12/2021" @default.
- W4200608399 hasLocation W42006083991 @default.
- W4200608399 hasLocation W42006083992 @default.
- W4200608399 hasOpenAccess W4200608399 @default.
- W4200608399 hasPrimaryLocation W42006083991 @default.
- W4200608399 hasRelatedWork W2027425545 @default.
- W4200608399 hasRelatedWork W2095961354 @default.
- W4200608399 hasRelatedWork W2163903634 @default.
- W4200608399 hasRelatedWork W2351995062 @default.
- W4200608399 hasRelatedWork W2392629839 @default.
- W4200608399 hasRelatedWork W2477540175 @default.
- W4200608399 hasRelatedWork W2739179464 @default.
- W4200608399 hasRelatedWork W2974002513 @default.
- W4200608399 hasRelatedWork W4200608399 @default.
- W4200608399 hasRelatedWork W57856700 @default.
- W4200608399 hasVolume "67" @default.
- W4200608399 isParatext "false" @default.
- W4200608399 isRetracted "false" @default.
- W4200608399 workType "article" @default.