FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200621887> ?p ?o ?g. }

• W4200621887 abstract "Abstract Background Peanut stem rot is a serious plant disease that causes great economic losses. At present, there are no effective measures to prevent or control the occurrence of this plant disease. Biological control is one of the most promising plant disease control measures. In this study, Pseudomonas chlororaphis subsp. aurantiaca strain zm-1, a bacterial strain with potential biocontrol properties isolated by our team from the rhizosphere soil of Anemarrhena asphodeloides , was studied to control this plant disease. Methods We prepared extracts of P. chlororaphis zm-1 extracellular antibacterial compounds (PECEs), determined their antifungal activities by confrontation assay, and identified their components by UPLC-MS/MS. The gene knockout strains were constructed by homologous recombination, and the biocontrol efficacy of P. chlororaphis zm-1 and its mutant strains were evaluated by pot experiments under greenhouse conditions and plot experiments, respectively. Results P. chlororaphis zm-1 could produce extracellular antifungal substances and inhibit the growth of Sclerotium rolfsii , the main pathogenic fungus causing peanut stem rot. The components of PECEs identified by UPLC-MS/MS showed that three kinds of phenazine compounds, i.e., 1-hydroxyphenazine, phenazine-1-carboxylic acid (PCA), and the core phenazine, were the principal components. In particular, 1-hydroxyphenazine produced by P. chlororaphis zm-1 showed antifungal activities against S. rolfsii , but the previously reported 2-hydroxyphenazine did not. The extracellular compounds of two mutant strains, Δ phzH and Δ phzE , was analysed and showed that Δ phzE did not produce any phenazine compounds, and Δ phzH no longer produced 1-hydroxyphenazine but could still produce PCA and phenazine. Furthermore, the antagonistic ability of Δ phzH declined, and that of Δ phzE was almost completely abolished. According to the results of pot experiments under greenhouse conditions, the biocontrol efficacy of Δ phzH dramatically declined to 47.21% compared with that of wild-type P. chlororaphis zm-1 (75.63%). Moreover, Δ phzE almost completely lost its ability to inhibit S. rolfsii (its biocontrol efficacy was reduced to 6.19%). The results of the larger plot experiments were also consistent with these results. Conclusions P. chlororaphis zm-1 has the potential to prevent and control peanut stem rot disease. Phenazines produced and secreted by P. chlororaphis zm-1 play a key role in the control of peanut stem rot caused by S. rolfsii . These findings provide a new idea for the effective prevention and treatment of peanut stem rot." @default.
• W4200621887 created "2021-12-31" @default.
• W4200621887 creator A5004241428 @default.
• W4200621887 creator A5004502972 @default.
• W4200621887 creator A5017335378 @default.
• W4200621887 creator A5030921116 @default.
• W4200621887 creator A5051981465 @default.
• W4200621887 creator A5072603002 @default.
• W4200621887 creator A5076946330 @default.
• W4200621887 date "2021-11-05" @default.
• W4200621887 modified "2023-09-27" @default.
• W4200621887 title "Characteristics of biological control and mechanisms of Pseudomonas chlororaphis zm-1 against peanut stem rot" @default.
• W4200621887 doi "https://doi.org/10.21203/rs.3.rs-966091/v1" @default.
• W4200621887 hasPublicationYear "2021" @default.
• W4200621887 type Work @default.
• W4200621887 citedByCount "0" @default.
• W4200621887 crossrefType "posted-content" @default.
• W4200621887 hasAuthorship W4200621887A5004241428 @default.
• W4200621887 hasAuthorship W4200621887A5004502972 @default.
• W4200621887 hasAuthorship W4200621887A5017335378 @default.
• W4200621887 hasAuthorship W4200621887A5030921116 @default.
• W4200621887 hasAuthorship W4200621887A5051981465 @default.
• W4200621887 hasAuthorship W4200621887A5072603002 @default.
• W4200621887 hasAuthorship W4200621887A5076946330 @default.
• W4200621887 hasBestOaLocation W42006218871 @default.
• W4200621887 hasConcept C144027150 @default.
• W4200621887 hasConcept C2777040454 @default.
• W4200621887 hasConcept C2778774814 @default.
• W4200621887 hasConcept C2780476228 @default.
• W4200621887 hasConcept C2780624194 @default.
• W4200621887 hasConcept C2781463711 @default.
• W4200621887 hasConcept C523546767 @default.
• W4200621887 hasConcept C54355233 @default.
• W4200621887 hasConcept C59822182 @default.
• W4200621887 hasConcept C74600772 @default.
• W4200621887 hasConcept C86626682 @default.
• W4200621887 hasConcept C86803240 @default.
• W4200621887 hasConcept C89423630 @default.
• W4200621887 hasConceptScore W4200621887C144027150 @default.
• W4200621887 hasConceptScore W4200621887C2777040454 @default.
• W4200621887 hasConceptScore W4200621887C2778774814 @default.
• W4200621887 hasConceptScore W4200621887C2780476228 @default.
• W4200621887 hasConceptScore W4200621887C2780624194 @default.
• W4200621887 hasConceptScore W4200621887C2781463711 @default.
• W4200621887 hasConceptScore W4200621887C523546767 @default.
• W4200621887 hasConceptScore W4200621887C54355233 @default.
• W4200621887 hasConceptScore W4200621887C59822182 @default.
• W4200621887 hasConceptScore W4200621887C74600772 @default.
• W4200621887 hasConceptScore W4200621887C86626682 @default.
• W4200621887 hasConceptScore W4200621887C86803240 @default.
• W4200621887 hasConceptScore W4200621887C89423630 @default.
• W4200621887 hasLocation W42006218871 @default.
• W4200621887 hasOpenAccess W4200621887 @default.
• W4200621887 hasPrimaryLocation W42006218871 @default.
• W4200621887 hasRelatedWork W119874814 @default.
• W4200621887 hasRelatedWork W2041897324 @default.
• W4200621887 hasRelatedWork W2185543632 @default.
• W4200621887 hasRelatedWork W2331412625 @default.
• W4200621887 hasRelatedWork W2587959696 @default.
• W4200621887 hasRelatedWork W2774174923 @default.
• W4200621887 hasRelatedWork W3091438935 @default.
• W4200621887 hasRelatedWork W3116654428 @default.
• W4200621887 hasRelatedWork W3192654908 @default.
• W4200621887 hasRelatedWork W4200621887 @default.
• W4200621887 isParatext "false" @default.
• W4200621887 isRetracted "false" @default.
• W4200621887 workType "article" @default.