Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205120815> ?p ?o ?g. }
- W4205120815 endingPage "106044" @default.
- W4205120815 startingPage "106044" @default.
- W4205120815 abstract "This work reports the immobilization of a fibrinolytic protease (FP) from Mucor subtilissimus UCP 1262 on Fe3O4 magnetic nanoparticles (MNPs) produced by precipitation of FeCl3·6H2O and FeCl2·4H2O, coated with polyaniline and activated with glutaraldehyde. The FP was obtained by solid state fermentation, precipitated with 40-60% ammonium sulfate, and purified by DEAE-Sephadex A50 ion exchange chromatography. The FP immobilization procedure allowed for an enzyme retention of 52.13%. The fibrinolytic protease immobilized on magnetic nanoparticles (MNPs/FP) maintained more than 60% of activity at a temperature of 40 to 60 °C and at pH 7 to 10, when compared to the non-immobilized enzyme. MNPs and MNPs/FP did not show any cytotoxicity against HEK-293 and J774A.1 cells. MNPs/FP was not hemolytic and reduced the hemolysis induced by MNPs from 2.07% to 1.37%. Thrombus degradation by MNPs/FP demonstrated that the immobilization process guaranteed the thrombolytic activity of the enzyme. MNPs/FP showed a total degradation of the γ chain of human fibrinogen within 90 min. These results suggest that MNPs/FP may be used as an alternative strategy to treat cardiovascular diseases with a targeted release through an external magnetic field." @default.
- W4205120815 created "2022-01-26" @default.
- W4205120815 creator A5000435645 @default.
- W4205120815 creator A5007837472 @default.
- W4205120815 creator A5010262841 @default.
- W4205120815 creator A5015934081 @default.
- W4205120815 creator A5029985858 @default.
- W4205120815 creator A5030288447 @default.
- W4205120815 creator A5034398759 @default.
- W4205120815 creator A5035506286 @default.
- W4205120815 creator A5042047450 @default.
- W4205120815 creator A5054445203 @default.
- W4205120815 creator A5063776613 @default.
- W4205120815 creator A5064922076 @default.
- W4205120815 creator A5079347031 @default.
- W4205120815 creator A5086536254 @default.
- W4205120815 date "2022-04-01" @default.
- W4205120815 modified "2023-10-10" @default.
- W4205120815 title "Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles" @default.
- W4205120815 cites W1817123886 @default.
- W4205120815 cites W1973228336 @default.
- W4205120815 cites W1973809917 @default.
- W4205120815 cites W1974694242 @default.
- W4205120815 cites W1976462184 @default.
- W4205120815 cites W2007326548 @default.
- W4205120815 cites W2033901709 @default.
- W4205120815 cites W2034039747 @default.
- W4205120815 cites W2036980354 @default.
- W4205120815 cites W2037929136 @default.
- W4205120815 cites W2043451183 @default.
- W4205120815 cites W2045112474 @default.
- W4205120815 cites W2049293261 @default.
- W4205120815 cites W2053006484 @default.
- W4205120815 cites W2056208210 @default.
- W4205120815 cites W2071659125 @default.
- W4205120815 cites W2080617670 @default.
- W4205120815 cites W2089693485 @default.
- W4205120815 cites W2092651769 @default.
- W4205120815 cites W2092822462 @default.
- W4205120815 cites W2102552259 @default.
- W4205120815 cites W2107301824 @default.
- W4205120815 cites W2114918609 @default.
- W4205120815 cites W2126915932 @default.
- W4205120815 cites W2148562097 @default.
- W4205120815 cites W2156778371 @default.
- W4205120815 cites W2342995846 @default.
- W4205120815 cites W2394824555 @default.
- W4205120815 cites W2409459743 @default.
- W4205120815 cites W2488554348 @default.
- W4205120815 cites W2586687844 @default.
- W4205120815 cites W2612221010 @default.
- W4205120815 cites W2619353200 @default.
- W4205120815 cites W2752312135 @default.
- W4205120815 cites W2774266731 @default.
- W4205120815 cites W2785597087 @default.
- W4205120815 cites W2789864546 @default.
- W4205120815 cites W2792511810 @default.
- W4205120815 cites W2793124989 @default.
- W4205120815 cites W2794849029 @default.
- W4205120815 cites W2795690488 @default.
- W4205120815 cites W2889824478 @default.
- W4205120815 cites W2900017138 @default.
- W4205120815 cites W2901372639 @default.
- W4205120815 cites W2902912616 @default.
- W4205120815 cites W2904097792 @default.
- W4205120815 cites W2915298649 @default.
- W4205120815 cites W2920895847 @default.
- W4205120815 cites W2955864276 @default.
- W4205120815 cites W2962012440 @default.
- W4205120815 cites W3003715004 @default.
- W4205120815 cites W3011234123 @default.
- W4205120815 doi "https://doi.org/10.1016/j.pep.2022.106044" @default.
- W4205120815 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34998976" @default.
- W4205120815 hasPublicationYear "2022" @default.
- W4205120815 type Work @default.
- W4205120815 citedByCount "3" @default.
- W4205120815 countsByYear W42051208152023 @default.
- W4205120815 crossrefType "journal-article" @default.
- W4205120815 hasAuthorship W4205120815A5000435645 @default.
- W4205120815 hasAuthorship W4205120815A5007837472 @default.
- W4205120815 hasAuthorship W4205120815A5010262841 @default.
- W4205120815 hasAuthorship W4205120815A5015934081 @default.
- W4205120815 hasAuthorship W4205120815A5029985858 @default.
- W4205120815 hasAuthorship W4205120815A5030288447 @default.
- W4205120815 hasAuthorship W4205120815A5034398759 @default.
- W4205120815 hasAuthorship W4205120815A5035506286 @default.
- W4205120815 hasAuthorship W4205120815A5042047450 @default.
- W4205120815 hasAuthorship W4205120815A5054445203 @default.
- W4205120815 hasAuthorship W4205120815A5063776613 @default.
- W4205120815 hasAuthorship W4205120815A5064922076 @default.
- W4205120815 hasAuthorship W4205120815A5079347031 @default.
- W4205120815 hasAuthorship W4205120815A5086536254 @default.
- W4205120815 hasConcept C13965031 @default.
- W4205120815 hasConcept C155672457 @default.
- W4205120815 hasConcept C171250308 @default.
- W4205120815 hasConcept C181199279 @default.
- W4205120815 hasConcept C185592680 @default.
- W4205120815 hasConcept C192562407 @default.