FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205142613> ?p ?o ?g. }

• W4205142613 endingPage "301" @default.
• W4205142613 startingPage "301" @default.
• W4205142613 abstract "Doxorubicin (Dox) is an anthracycline chemotherapeutic agent used to treat breast, leukemia, and lymphoma malignancies. However, cardiotoxicity and inherent acquired resistance are major drawbacks, limiting its clinical application. We have previously shown that cyclic peptide [WR]9 containing alternate tryptophan (W) and arginine (R) residues acts as an efficient molecular transporter. An amphiphilic cyclic peptide containing a lysine (K) residue and alternative W and R was conjugated through a free side chain amino group with Dox via a glutarate linker to afford [(WR)8WKβA]-Dox conjugate. Antiproliferative assays were performed in different cancer cell lines using the conjugate and the corresponding physical mixture of the peptide and Dox to evaluate the effectiveness of synthesized conjugate compared to the parent drug alone. [(WR)8WKβA]-Dox conjugate showed higher antiproliferative activity at 10 µM and 5 µM than Dox alone at 5 μM. The conjugate inhibited the cell viability of ovarian adenocarcinoma (SK-OV-3) by 59% and the triple-negative breast cancer cells MDA-MB-231 and MCF-7 by 71% and 77%, respectively, at a concentration of 5 μM after 72 h of incubation. In contrast, Dox inhibited the proliferation of SK-OV-3, MDA-MB-231, and MCF-7 by 35%, 63%, and 57%, respectively. Furthermore, [(WR)8WKβA]-Dox conjugate (5 µM) inhibited the cell viability of Dox-resistant cells (MES-SA/MX2) by 92%, while the viability of cells incubated with free Dox was only 15% at 5 μM. Confocal microscopy images confirmed the ability of both Dox conjugate and the physical mixture of the peptide with the drug to deliver Dox through an endocytosis-independent pathway, as the uptake was not inhibited in the presence of endocytosis inhibitors. The stability of Dox conjugate was observed at different time intervals using analytical HPLC when the conjugate was incubated with 25% human serum. Half-life (t1/2) for [(WR)8WKβA]-Dox conjugate was (∼6 h), and more than 80% of the conjugate was degraded at 12 h. The release of free Dox was assessed intracellularly using the CCRF-CEM cell line. The experiment demonstrated that approximately 100% of free Dox was released from the conjugate intracellularly within 72 h. These data confirm the ability of the cyclic cell-penetrating peptide containing tryptophan and arginine residues as an efficient tool for delivery of Dox and for overcoming resistance to it." @default.
• W4205142613 created "2022-01-26" @default.
• W4205142613 creator A5016314740 @default.
• W4205142613 creator A5031842435 @default.
• W4205142613 creator A5033339559 @default.
• W4205142613 creator A5060177451 @default.
• W4205142613 date "2022-01-16" @default.
• W4205142613 modified "2023-10-14" @default.
• W4205142613 title "[(WR)8WKβA]-Doxorubicin Conjugate: A Delivery System to Overcome Multi-Drug Resistance against Doxorubicin" @default.
• W4205142613 cites W1533545977 @default.
• W4205142613 cites W1584683177 @default.
• W4205142613 cites W1825085207 @default.
• W4205142613 cites W1964180981 @default.
• W4205142613 cites W1974064124 @default.
• W4205142613 cites W1974695334 @default.
• W4205142613 cites W1980441078 @default.
• W4205142613 cites W1985126638 @default.
• W4205142613 cites W1998000924 @default.
• W4205142613 cites W2003032820 @default.
• W4205142613 cites W2009124763 @default.
• W4205142613 cites W2015704490 @default.
• W4205142613 cites W2025152609 @default.
• W4205142613 cites W2031606126 @default.
• W4205142613 cites W2034371347 @default.
• W4205142613 cites W2038720675 @default.
• W4205142613 cites W2042015955 @default.
• W4205142613 cites W2044164984 @default.
• W4205142613 cites W2055904567 @default.
• W4205142613 cites W2062063474 @default.
• W4205142613 cites W2064282122 @default.
• W4205142613 cites W2067794181 @default.
• W4205142613 cites W2083466846 @default.
• W4205142613 cites W2089333071 @default.
• W4205142613 cites W2097202791 @default.
• W4205142613 cites W2118425554 @default.
• W4205142613 cites W2123735085 @default.
• W4205142613 cites W2130168443 @default.
• W4205142613 cites W2139436990 @default.
• W4205142613 cites W2146039804 @default.
• W4205142613 cites W2155001082 @default.
• W4205142613 cites W2162689981 @default.
• W4205142613 cites W2166618356 @default.
• W4205142613 cites W2169302611 @default.
• W4205142613 cites W2301188205 @default.
• W4205142613 cites W2334523147 @default.
• W4205142613 cites W2444240157 @default.
• W4205142613 cites W2472306545 @default.
• W4205142613 cites W2555627105 @default.
• W4205142613 cites W2766181274 @default.
• W4205142613 cites W2810037075 @default.
• W4205142613 cites W2902903020 @default.
• W4205142613 cites W2939519670 @default.
• W4205142613 cites W2971924556 @default.
• W4205142613 cites W3116766475 @default.
• W4205142613 cites W3160601127 @default.
• W4205142613 cites W3200517152 @default.
• W4205142613 cites W4239761639 @default.
• W4205142613 doi "https://doi.org/10.3390/cells11020301" @default.
• W4205142613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35053417" @default.
• W4205142613 hasPublicationYear "2022" @default.
• W4205142613 type Work @default.
• W4205142613 citedByCount "7" @default.
• W4205142613 countsByYear W42051426132022 @default.
• W4205142613 countsByYear W42051426132023 @default.
• W4205142613 crossrefType "journal-article" @default.
• W4205142613 hasAuthorship W4205142613A5016314740 @default.
• W4205142613 hasAuthorship W4205142613A5031842435 @default.
• W4205142613 hasAuthorship W4205142613A5033339559 @default.
• W4205142613 hasAuthorship W4205142613A5060177451 @default.
• W4205142613 hasBestOaLocation W42051426131 @default.
• W4205142613 hasConcept C109316439 @default.
• W4205142613 hasConcept C134306372 @default.
• W4205142613 hasConcept C178790620 @default.
• W4205142613 hasConcept C185592680 @default.
• W4205142613 hasConcept C197336794 @default.
• W4205142613 hasConcept C202751555 @default.
• W4205142613 hasConcept C2776260442 @default.
• W4205142613 hasConcept C2776694085 @default.
• W4205142613 hasConcept C2779820397 @default.
• W4205142613 hasConcept C2781303535 @default.
• W4205142613 hasConcept C33923547 @default.
• W4205142613 hasConcept C53227056 @default.
• W4205142613 hasConcept C54355233 @default.
• W4205142613 hasConcept C55493867 @default.
• W4205142613 hasConcept C86803240 @default.
• W4205142613 hasConcept C98274493 @default.
• W4205142613 hasConceptScore W4205142613C109316439 @default.
• W4205142613 hasConceptScore W4205142613C134306372 @default.
• W4205142613 hasConceptScore W4205142613C178790620 @default.
• W4205142613 hasConceptScore W4205142613C185592680 @default.
• W4205142613 hasConceptScore W4205142613C197336794 @default.
• W4205142613 hasConceptScore W4205142613C202751555 @default.
• W4205142613 hasConceptScore W4205142613C2776260442 @default.
• W4205142613 hasConceptScore W4205142613C2776694085 @default.
• W4205142613 hasConceptScore W4205142613C2779820397 @default.
• W4205142613 hasConceptScore W4205142613C2781303535 @default.
• W4205142613 hasConceptScore W4205142613C33923547 @default.
• W4205142613 hasConceptScore W4205142613C53227056 @default.

• next