Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205156964> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W4205156964 abstract "The prototype fexA gene confers combined resistance to chloramphenicol and florfenicol. However, fexA variants mediating resistance only to chloramphenicol have been identified, such as in the case of a Staphylococcus aureus isolate recovered from poultry meat illegally imported to Germany. The effects of the individual mutations detected in the fexA sequence of this isolate were investigated in this study. A total of 11 fexA variants, including prototype fexA and variants containing the different previously described mutations either alone or in different combinations, were generated by on-chip gene synthesis and site-directed mutagenesis. The constructs were inserted into a shuttle vector and transformed into three recipient strains (Escherichia coli, Staphylococcus aureus, and Salmonella Typhimurium). Subsequently, minimal inhibitory concentrations (MIC) of florfenicol and chloramphenicol were determined. In addition, protein modeling was used to predict the structural effects of the mutations. The lack of florfenicol-resistance mediating properties of the fexA variants could be attributed to the presence of a C110T and/or G98C mutation. Transformants carrying fexA variants containing either of these mutations, or both, showed a reduction of florfenicol MICs compared to those transformants carrying prototype fexA or any of the other variants. The significance of these mutations was supported by the generated protein models, indicating a substitution toward more voluminous amino-acids in the substrate-binding site of FexA. The remaining mutations, A391G and C961A, did not result in lower florfenicol-resistance compared to prototype fexA." @default.
- W4205156964 created "2022-01-26" @default.
- W4205156964 creator A5001173964 @default.
- W4205156964 creator A5007726682 @default.
- W4205156964 creator A5009505239 @default.
- W4205156964 creator A5050662903 @default.
- W4205156964 creator A5079572315 @default.
- W4205156964 date "2022-01-07" @default.
- W4205156964 modified "2023-09-25" @default.
- W4205156964 title "Mutations in the Phenicol Exporter Gene fexA Impact Resistance Levels in Three Bacterial Hosts According to Susceptibility Testing and Protein Modeling" @default.
- W4205156964 cites W1987087864 @default.
- W4205156964 cites W2038621083 @default.
- W4205156964 cites W2067910615 @default.
- W4205156964 cites W2088371041 @default.
- W4205156964 cites W2090971845 @default.
- W4205156964 cites W2098874489 @default.
- W4205156964 cites W2122082432 @default.
- W4205156964 cites W2124576329 @default.
- W4205156964 cites W2127248218 @default.
- W4205156964 cites W2145871643 @default.
- W4205156964 cites W2153453937 @default.
- W4205156964 cites W2161285490 @default.
- W4205156964 cites W2279892490 @default.
- W4205156964 cites W2418036698 @default.
- W4205156964 cites W2560266418 @default.
- W4205156964 cites W2902821158 @default.
- W4205156964 cites W3005708520 @default.
- W4205156964 cites W3046197522 @default.
- W4205156964 cites W3177828909 @default.
- W4205156964 cites W3206384650 @default.
- W4205156964 doi "https://doi.org/10.3389/fmicb.2021.794435" @default.
- W4205156964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35069492" @default.
- W4205156964 hasPublicationYear "2022" @default.
- W4205156964 type Work @default.
- W4205156964 citedByCount "1" @default.
- W4205156964 countsByYear W42051569642022 @default.
- W4205156964 crossrefType "journal-article" @default.
- W4205156964 hasAuthorship W4205156964A5001173964 @default.
- W4205156964 hasAuthorship W4205156964A5007726682 @default.
- W4205156964 hasAuthorship W4205156964A5009505239 @default.
- W4205156964 hasAuthorship W4205156964A5050662903 @default.
- W4205156964 hasAuthorship W4205156964A5079572315 @default.
- W4205156964 hasBestOaLocation W42051569641 @default.
- W4205156964 hasConcept C104317684 @default.
- W4205156964 hasConcept C145640855 @default.
- W4205156964 hasConcept C16318435 @default.
- W4205156964 hasConcept C2778473333 @default.
- W4205156964 hasConcept C2779489039 @default.
- W4205156964 hasConcept C2780663544 @default.
- W4205156964 hasConcept C2781065037 @default.
- W4205156964 hasConcept C40767141 @default.
- W4205156964 hasConcept C501593827 @default.
- W4205156964 hasConcept C501734568 @default.
- W4205156964 hasConcept C523546767 @default.
- W4205156964 hasConcept C54355233 @default.
- W4205156964 hasConcept C547475151 @default.
- W4205156964 hasConcept C86803240 @default.
- W4205156964 hasConcept C89423630 @default.
- W4205156964 hasConcept C92087593 @default.
- W4205156964 hasConceptScore W4205156964C104317684 @default.
- W4205156964 hasConceptScore W4205156964C145640855 @default.
- W4205156964 hasConceptScore W4205156964C16318435 @default.
- W4205156964 hasConceptScore W4205156964C2778473333 @default.
- W4205156964 hasConceptScore W4205156964C2779489039 @default.
- W4205156964 hasConceptScore W4205156964C2780663544 @default.
- W4205156964 hasConceptScore W4205156964C2781065037 @default.
- W4205156964 hasConceptScore W4205156964C40767141 @default.
- W4205156964 hasConceptScore W4205156964C501593827 @default.
- W4205156964 hasConceptScore W4205156964C501734568 @default.
- W4205156964 hasConceptScore W4205156964C523546767 @default.
- W4205156964 hasConceptScore W4205156964C54355233 @default.
- W4205156964 hasConceptScore W4205156964C547475151 @default.
- W4205156964 hasConceptScore W4205156964C86803240 @default.
- W4205156964 hasConceptScore W4205156964C89423630 @default.
- W4205156964 hasConceptScore W4205156964C92087593 @default.
- W4205156964 hasLocation W42051569641 @default.
- W4205156964 hasLocation W42051569642 @default.
- W4205156964 hasLocation W42051569643 @default.
- W4205156964 hasLocation W42051569644 @default.
- W4205156964 hasOpenAccess W4205156964 @default.
- W4205156964 hasPrimaryLocation W42051569641 @default.
- W4205156964 hasRelatedWork W1969328010 @default.
- W4205156964 hasRelatedWork W2009647017 @default.
- W4205156964 hasRelatedWork W2077490993 @default.
- W4205156964 hasRelatedWork W2133882122 @default.
- W4205156964 hasRelatedWork W2148762476 @default.
- W4205156964 hasRelatedWork W2313387403 @default.
- W4205156964 hasRelatedWork W2374936410 @default.
- W4205156964 hasRelatedWork W2383245328 @default.
- W4205156964 hasRelatedWork W2618383668 @default.
- W4205156964 hasRelatedWork W2941294170 @default.
- W4205156964 hasVolume "12" @default.
- W4205156964 isParatext "false" @default.
- W4205156964 isRetracted "false" @default.
- W4205156964 workType "article" @default.