Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205255717> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4205255717 endingPage "101101" @default.
- W4205255717 startingPage "101101" @default.
- W4205255717 abstract "The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to isolate high-affinity nanobodies from common framework libraries. This approach involves shuffling the CDRs of low-affinity variants during the sorting of yeast-displayed libraries to directly isolate high-affinity nanobodies without the need for lead isolation and optimization. We expect this approach, which we demonstrate for SARS-CoV-2 neutralizing nanobodies, will simplify the generation of high-affinity nanobodies. For complete details on the use and execution of this profile, please refer to Zupancic et al. (2021)." @default.
- W4205255717 created "2022-01-26" @default.
- W4205255717 creator A5029310606 @default.
- W4205255717 creator A5032830604 @default.
- W4205255717 creator A5065340545 @default.
- W4205255717 date "2022-03-01" @default.
- W4205255717 modified "2023-10-11" @default.
- W4205255717 title "Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis" @default.
- W4205255717 cites W2107409328 @default.
- W4205255717 cites W2786832567 @default.
- W4205255717 cites W2890351601 @default.
- W4205255717 cites W2928162137 @default.
- W4205255717 cites W3135072281 @default.
- W4205255717 cites W3173700036 @default.
- W4205255717 doi "https://doi.org/10.1016/j.xpro.2021.101101" @default.
- W4205255717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35098159" @default.
- W4205255717 hasPublicationYear "2022" @default.
- W4205255717 type Work @default.
- W4205255717 citedByCount "4" @default.
- W4205255717 countsByYear W42052557172022 @default.
- W4205255717 countsByYear W42052557172023 @default.
- W4205255717 crossrefType "journal-article" @default.
- W4205255717 hasAuthorship W4205255717A5029310606 @default.
- W4205255717 hasAuthorship W4205255717A5032830604 @default.
- W4205255717 hasAuthorship W4205255717A5065340545 @default.
- W4205255717 hasBestOaLocation W42052557171 @default.
- W4205255717 hasConcept C104317684 @default.
- W4205255717 hasConcept C147816474 @default.
- W4205255717 hasConcept C159654299 @default.
- W4205255717 hasConcept C16318435 @default.
- W4205255717 hasConcept C181199279 @default.
- W4205255717 hasConcept C185592680 @default.
- W4205255717 hasConcept C202269582 @default.
- W4205255717 hasConcept C2781377902 @default.
- W4205255717 hasConcept C41008148 @default.
- W4205255717 hasConcept C501734568 @default.
- W4205255717 hasConcept C54355233 @default.
- W4205255717 hasConcept C55493867 @default.
- W4205255717 hasConcept C70721500 @default.
- W4205255717 hasConcept C86803240 @default.
- W4205255717 hasConceptScore W4205255717C104317684 @default.
- W4205255717 hasConceptScore W4205255717C147816474 @default.
- W4205255717 hasConceptScore W4205255717C159654299 @default.
- W4205255717 hasConceptScore W4205255717C16318435 @default.
- W4205255717 hasConceptScore W4205255717C181199279 @default.
- W4205255717 hasConceptScore W4205255717C185592680 @default.
- W4205255717 hasConceptScore W4205255717C202269582 @default.
- W4205255717 hasConceptScore W4205255717C2781377902 @default.
- W4205255717 hasConceptScore W4205255717C41008148 @default.
- W4205255717 hasConceptScore W4205255717C501734568 @default.
- W4205255717 hasConceptScore W4205255717C54355233 @default.
- W4205255717 hasConceptScore W4205255717C55493867 @default.
- W4205255717 hasConceptScore W4205255717C70721500 @default.
- W4205255717 hasConceptScore W4205255717C86803240 @default.
- W4205255717 hasIssue "1" @default.
- W4205255717 hasLocation W42052557171 @default.
- W4205255717 hasLocation W42052557172 @default.
- W4205255717 hasLocation W42052557173 @default.
- W4205255717 hasLocation W42052557174 @default.
- W4205255717 hasOpenAccess W4205255717 @default.
- W4205255717 hasPrimaryLocation W42052557171 @default.
- W4205255717 hasRelatedWork W1983164158 @default.
- W4205255717 hasRelatedWork W2018295245 @default.
- W4205255717 hasRelatedWork W2090914505 @default.
- W4205255717 hasRelatedWork W2403301484 @default.
- W4205255717 hasRelatedWork W2478713127 @default.
- W4205255717 hasRelatedWork W2502312529 @default.
- W4205255717 hasRelatedWork W2885204620 @default.
- W4205255717 hasRelatedWork W53478023 @default.
- W4205255717 hasRelatedWork W1977887826 @default.
- W4205255717 hasRelatedWork W2521293589 @default.
- W4205255717 hasVolume "3" @default.
- W4205255717 isParatext "false" @default.
- W4205255717 isRetracted "false" @default.
- W4205255717 workType "article" @default.