Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205399285> ?p ?o ?g. }
- W4205399285 abstract "Rhamnogalacturonan lyase (RGL) cleaves backbone α-1,4 glycosidic bonds between L-rhamnose and D-galacturonic acid residues in type I rhamnogalacturonan (RG-I) by β-elimination to generate RG oligosaccharides with various degrees of polymerization. Here, we cloned, expressed, purified and biochemically characterized two RGLs (Bo3128 and Bo4416) in the PL11 family from Bacteroides ovatus ATCC 8483. Bo3128 and Bo4416 displayed maximal activity at pH 9.5 and pH 6.5, respectively. Whereas the activity of Bo3128 could be increased 1.5 fold in the presence of 5 mM Ca2+, Bo4416 required divalent metal ions to show any enzymatic activity. Both of RGLs showed a substrate preference for RG-I compared to other pectin domains. Bo4416 and Bo3128 primarily yielded unsaturated RG oligosaccharides, with Bo3128 also producing them with short side chains, with yields of 32.4 and 62.4%, respectively. Characterization of both RGLs contribute to the preparation of rhamnogalacturonan oligosaccharides, as well as for the analysis of the fine structure of RG-I pectins." @default.
- W4205399285 created "2022-01-25" @default.
- W4205399285 creator A5001922805 @default.
- W4205399285 creator A5005071062 @default.
- W4205399285 creator A5008943595 @default.
- W4205399285 creator A5011713074 @default.
- W4205399285 creator A5012810451 @default.
- W4205399285 creator A5013791721 @default.
- W4205399285 creator A5031890019 @default.
- W4205399285 creator A5035702264 @default.
- W4205399285 creator A5042429449 @default.
- W4205399285 date "2022-01-11" @default.
- W4205399285 modified "2023-10-05" @default.
- W4205399285 title "Biochemical Characterization of Two Rhamnogalacturonan Lyases From Bacteroides ovatus ATCC 8483 With Preference for RG-I Substrates" @default.
- W4205399285 cites W1640611800 @default.
- W4205399285 cites W1981206470 @default.
- W4205399285 cites W1981361900 @default.
- W4205399285 cites W1994423695 @default.
- W4205399285 cites W1998022085 @default.
- W4205399285 cites W1998783353 @default.
- W4205399285 cites W1999577767 @default.
- W4205399285 cites W2012912350 @default.
- W4205399285 cites W2022395923 @default.
- W4205399285 cites W2029740040 @default.
- W4205399285 cites W2062549379 @default.
- W4205399285 cites W2071280215 @default.
- W4205399285 cites W2084751582 @default.
- W4205399285 cites W2087409461 @default.
- W4205399285 cites W2102587353 @default.
- W4205399285 cites W2104046482 @default.
- W4205399285 cites W2111090332 @default.
- W4205399285 cites W2115206422 @default.
- W4205399285 cites W2117306479 @default.
- W4205399285 cites W2118277235 @default.
- W4205399285 cites W2121091188 @default.
- W4205399285 cites W2134807027 @default.
- W4205399285 cites W2150675192 @default.
- W4205399285 cites W2157407884 @default.
- W4205399285 cites W2158794108 @default.
- W4205399285 cites W2170687446 @default.
- W4205399285 cites W2171309029 @default.
- W4205399285 cites W2289787696 @default.
- W4205399285 cites W2559730022 @default.
- W4205399285 cites W2763947858 @default.
- W4205399285 cites W2772940414 @default.
- W4205399285 cites W2891485726 @default.
- W4205399285 cites W2892169121 @default.
- W4205399285 cites W2910300811 @default.
- W4205399285 cites W2954920564 @default.
- W4205399285 cites W2990056741 @default.
- W4205399285 cites W3035129734 @default.
- W4205399285 cites W3043289170 @default.
- W4205399285 cites W3086512668 @default.
- W4205399285 cites W3163851623 @default.
- W4205399285 cites W4245461700 @default.
- W4205399285 doi "https://doi.org/10.3389/fmicb.2021.799875" @default.
- W4205399285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35087500" @default.
- W4205399285 hasPublicationYear "2022" @default.
- W4205399285 type Work @default.
- W4205399285 citedByCount "2" @default.
- W4205399285 countsByYear W42053992852022 @default.
- W4205399285 countsByYear W42053992852023 @default.
- W4205399285 crossrefType "journal-article" @default.
- W4205399285 hasAuthorship W4205399285A5001922805 @default.
- W4205399285 hasAuthorship W4205399285A5005071062 @default.
- W4205399285 hasAuthorship W4205399285A5008943595 @default.
- W4205399285 hasAuthorship W4205399285A5011713074 @default.
- W4205399285 hasAuthorship W4205399285A5012810451 @default.
- W4205399285 hasAuthorship W4205399285A5013791721 @default.
- W4205399285 hasAuthorship W4205399285A5031890019 @default.
- W4205399285 hasAuthorship W4205399285A5035702264 @default.
- W4205399285 hasAuthorship W4205399285A5042429449 @default.
- W4205399285 hasBestOaLocation W42053992851 @default.
- W4205399285 hasConcept C150487720 @default.
- W4205399285 hasConcept C178790620 @default.
- W4205399285 hasConcept C181199279 @default.
- W4205399285 hasConcept C185592680 @default.
- W4205399285 hasConcept C18903297 @default.
- W4205399285 hasConcept C2777289219 @default.
- W4205399285 hasConcept C2778168646 @default.
- W4205399285 hasConcept C523546767 @default.
- W4205399285 hasConcept C54355233 @default.
- W4205399285 hasConcept C55493867 @default.
- W4205399285 hasConcept C71240020 @default.
- W4205399285 hasConcept C74884574 @default.
- W4205399285 hasConcept C86803240 @default.
- W4205399285 hasConceptScore W4205399285C150487720 @default.
- W4205399285 hasConceptScore W4205399285C178790620 @default.
- W4205399285 hasConceptScore W4205399285C181199279 @default.
- W4205399285 hasConceptScore W4205399285C185592680 @default.
- W4205399285 hasConceptScore W4205399285C18903297 @default.
- W4205399285 hasConceptScore W4205399285C2777289219 @default.
- W4205399285 hasConceptScore W4205399285C2778168646 @default.
- W4205399285 hasConceptScore W4205399285C523546767 @default.
- W4205399285 hasConceptScore W4205399285C54355233 @default.
- W4205399285 hasConceptScore W4205399285C55493867 @default.
- W4205399285 hasConceptScore W4205399285C71240020 @default.
- W4205399285 hasConceptScore W4205399285C74884574 @default.
- W4205399285 hasConceptScore W4205399285C86803240 @default.
- W4205399285 hasFunder F4320321001 @default.