FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205478965> ?p ?o ?g. }

• W4205478965 abstract "Canine distemper virus (CDV), which is highly infectious, has caused outbreaks of varying scales in domestic and wild animals worldwide, so the development of a high-efficiency vaccine has broad application prospects. Currently, the commercial vaccine of CDV is an attenuated vaccine, which has the disadvantages of a complex preparation process, high cost and safety risk. It is necessary to develop a safe and effective CDV vaccine that is easy to produce on a large scale. In this study, sequences of CDV haemagglutinin (HA) from the Yanaka strain were aligned, and three potential linear sequences, termed YaH3, YaH4, and YaH5, were collected. To increase the immunogenicity of the epitopes, ferritin was employed as a self-assembling nanoparticle element. The ferritin-coupled forms were termed YaH3F, YaH4F, and YaH5F, respectively. A full-length HA sequence coupled with ferritin was also constructed as a DNA vaccine to compare the immunogenicity of nanoparticles in prokaryotic expression.The self-assembly morphology of the proteins from prokaryotic expression was verified by transmission electron microscopy. All the proteins self-assembled into nanoparticles. The expression of the DNA vaccine YaHF in HEK-293T cells was also confirmed in vitro. After subcutaneous injection of epitope nanoparticles or intramuscular injection of DNA YaHF, all vaccines induced strong serum titres, and long-term potency of antibodies in serum could be detected after 84 days. Strong anti-CDV neutralizing activities were observed in both the YaH4F group and YaHF group. According to antibody typing and cytokine detection, YaH4F can induce both Th1 and Th2 immune responses. The results of flow cytometry detection indicated that compared with the control group, all the immunogens elicited an increase in CD3. Simultaneously, the serum antibodies induced by YaH4F and YaHF could significantly enhance the ADCC effect compared with the control group, indicating that the antibodies in the serum effectively recognized the antigens on the cell surface and induced NK cells to kill infected cells directly.YaH4F self-assembling nanoparticle obtained by prokaryotic expression has no less of an immune effect than YaHF, and H4 has great potential to become a key target for the easy and rapid preparation of epitope vaccines." @default.
• W4205478965 created "2022-01-26" @default.
• W4205478965 creator A5044053727 @default.
• W4205478965 creator A5049085663 @default.
• W4205478965 creator A5051663366 @default.
• W4205478965 creator A5054699705 @default.
• W4205478965 creator A5061084605 @default.
• W4205478965 creator A5061757283 @default.
• W4205478965 creator A5068003384 @default.
• W4205478965 creator A5073353234 @default.
• W4205478965 creator A5075329922 @default.
• W4205478965 creator A5079021767 @default.
• W4205478965 creator A5079705327 @default.
• W4205478965 creator A5089562070 @default.
• W4205478965 creator A5090849020 @default.
• W4205478965 date "2022-01-10" @default.
• W4205478965 modified "2023-10-01" @default.
• W4205478965 title "Self-assembling ferritin nanoparticles coupled with linear sequences from canine distemper virus haemagglutinin protein elicit robust immune responses" @default.
• W4205478965 cites W1460910896 @default.
• W4205478965 cites W1526839960 @default.
• W4205478965 cites W1830154927 @default.
• W4205478965 cites W1832268633 @default.
• W4205478965 cites W1967431849 @default.
• W4205478965 cites W1970966964 @default.
• W4205478965 cites W1993568862 @default.
• W4205478965 cites W2002844537 @default.
• W4205478965 cites W2020016826 @default.
• W4205478965 cites W2021112819 @default.
• W4205478965 cites W2041649802 @default.
• W4205478965 cites W2071759593 @default.
• W4205478965 cites W2072794252 @default.
• W4205478965 cites W2074903174 @default.
• W4205478965 cites W2076228730 @default.
• W4205478965 cites W2086168958 @default.
• W4205478965 cites W2093096499 @default.
• W4205478965 cites W2110572977 @default.
• W4205478965 cites W2160397907 @default.
• W4205478965 cites W2357123482 @default.
• W4205478965 cites W2571363236 @default.
• W4205478965 cites W2585072088 @default.
• W4205478965 cites W2611314002 @default.
• W4205478965 cites W2790611816 @default.
• W4205478965 cites W2803384616 @default.
• W4205478965 cites W2911675607 @default.
• W4205478965 cites W2921040792 @default.
• W4205478965 cites W2922325963 @default.
• W4205478965 cites W2947966265 @default.
• W4205478965 cites W2995969395 @default.
• W4205478965 cites W3019605001 @default.
• W4205478965 cites W3020860985 @default.
• W4205478965 cites W3035071403 @default.
• W4205478965 cites W3092813154 @default.
• W4205478965 cites W3157093814 @default.
• W4205478965 cites W3165516549 @default.
• W4205478965 cites W3199281996 @default.
• W4205478965 cites W881350307 @default.
• W4205478965 cites W2341244163 @default.
• W4205478965 doi "https://doi.org/10.1186/s12951-021-01229-0" @default.
• W4205478965 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35012571" @default.
• W4205478965 hasPublicationYear "2022" @default.
• W4205478965 type Work @default.
• W4205478965 citedByCount "4" @default.
• W4205478965 countsByYear W42054789652022 @default.
• W4205478965 countsByYear W42054789652023 @default.
• W4205478965 crossrefType "journal-article" @default.
• W4205478965 hasAuthorship W4205478965A5044053727 @default.
• W4205478965 hasAuthorship W4205478965A5049085663 @default.
• W4205478965 hasAuthorship W4205478965A5051663366 @default.
• W4205478965 hasAuthorship W4205478965A5054699705 @default.
• W4205478965 hasAuthorship W4205478965A5061084605 @default.
• W4205478965 hasAuthorship W4205478965A5061757283 @default.
• W4205478965 hasAuthorship W4205478965A5068003384 @default.
• W4205478965 hasAuthorship W4205478965A5073353234 @default.
• W4205478965 hasAuthorship W4205478965A5075329922 @default.
• W4205478965 hasAuthorship W4205478965A5079021767 @default.
• W4205478965 hasAuthorship W4205478965A5079705327 @default.
• W4205478965 hasAuthorship W4205478965A5089562070 @default.
• W4205478965 hasAuthorship W4205478965A5090849020 @default.
• W4205478965 hasBestOaLocation W42054789651 @default.
• W4205478965 hasConcept C153911025 @default.
• W4205478965 hasConcept C159047783 @default.
• W4205478965 hasConcept C159654299 @default.
• W4205478965 hasConcept C167508917 @default.
• W4205478965 hasConcept C195616568 @default.
• W4205478965 hasConcept C203014093 @default.
• W4205478965 hasConcept C2522874641 @default.
• W4205478965 hasConcept C2776040883 @default.
• W4205478965 hasConcept C2779261636 @default.
• W4205478965 hasConcept C2780801004 @default.
• W4205478965 hasConcept C2780868878 @default.
• W4205478965 hasConcept C86803240 @default.
• W4205478965 hasConcept C8891405 @default.
• W4205478965 hasConceptScore W4205478965C153911025 @default.
• W4205478965 hasConceptScore W4205478965C159047783 @default.
• W4205478965 hasConceptScore W4205478965C159654299 @default.
• W4205478965 hasConceptScore W4205478965C167508917 @default.
• W4205478965 hasConceptScore W4205478965C195616568 @default.
• W4205478965 hasConceptScore W4205478965C203014093 @default.
• W4205478965 hasConceptScore W4205478965C2522874641 @default.
• W4205478965 hasConceptScore W4205478965C2776040883 @default.

• next