Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205500932> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4205500932 abstract "The serotonin transporter is a sodium and chloride-coupled transporter that pumps extracellular serotonin into cells. S-citalopram is a drug used to treat depression and anxiety by binding to the serotonin transporter with high-affinity, blocking serotonin reuptake. Here we report an efficient procedure and a set of tools to stabilize, express, purify, and crystallize serotonin transporter-antibody complexes bound to S-citalopram and other antidepressants. Mutations which stabilize the serotonin transporter were identified using an S-citalopram binding assay. Serotonin transporter expressed in baculovirus-transduced HEK293S GnTI- cells, was reconstituted into proteoliposomes and used to raise high-affinity antibodies. We have developed a strategy to discover antibodies that are useful for structural studies. A straightforward approach for the expression of antibody fragments in Sf9 cells has also been established. Transporter-antibody complexes purified using this procedure are well-behaved and readily crystallize, producing complexes with S-citalopram that diffract X-rays to 3-4 Å resolution. The strategies developed here can be utilized to determine the structure of other challenging membrane proteins." @default.
- W4205500932 created "2022-01-25" @default.
- W4205500932 creator A5008623516 @default.
- W4205500932 creator A5017505330 @default.
- W4205500932 creator A5088125571 @default.
- W4205500932 date "2016-11-27" @default.
- W4205500932 modified "2023-10-14" @default.
- W4205500932 title "Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to <em>S</em>-citalopram" @default.
- W4205500932 doi "https://doi.org/10.3791/54792-v" @default.
- W4205500932 hasPublicationYear "2016" @default.
- W4205500932 type Work @default.
- W4205500932 citedByCount "0" @default.
- W4205500932 crossrefType "journal-article" @default.
- W4205500932 hasAuthorship W4205500932A5008623516 @default.
- W4205500932 hasAuthorship W4205500932A5017505330 @default.
- W4205500932 hasAuthorship W4205500932A5088125571 @default.
- W4205500932 hasBestOaLocation W42055009321 @default.
- W4205500932 hasConcept C104317684 @default.
- W4205500932 hasConcept C149011108 @default.
- W4205500932 hasConcept C170493617 @default.
- W4205500932 hasConcept C185592680 @default.
- W4205500932 hasConcept C2775864247 @default.
- W4205500932 hasConcept C2776134451 @default.
- W4205500932 hasConcept C2777490545 @default.
- W4205500932 hasConcept C2778897192 @default.
- W4205500932 hasConcept C2780092697 @default.
- W4205500932 hasConcept C2908747647 @default.
- W4205500932 hasConcept C2910584757 @default.
- W4205500932 hasConcept C55493867 @default.
- W4205500932 hasConcept C86803240 @default.
- W4205500932 hasConcept C98274493 @default.
- W4205500932 hasConcept C99631742 @default.
- W4205500932 hasConceptScore W4205500932C104317684 @default.
- W4205500932 hasConceptScore W4205500932C149011108 @default.
- W4205500932 hasConceptScore W4205500932C170493617 @default.
- W4205500932 hasConceptScore W4205500932C185592680 @default.
- W4205500932 hasConceptScore W4205500932C2775864247 @default.
- W4205500932 hasConceptScore W4205500932C2776134451 @default.
- W4205500932 hasConceptScore W4205500932C2777490545 @default.
- W4205500932 hasConceptScore W4205500932C2778897192 @default.
- W4205500932 hasConceptScore W4205500932C2780092697 @default.
- W4205500932 hasConceptScore W4205500932C2908747647 @default.
- W4205500932 hasConceptScore W4205500932C2910584757 @default.
- W4205500932 hasConceptScore W4205500932C55493867 @default.
- W4205500932 hasConceptScore W4205500932C86803240 @default.
- W4205500932 hasConceptScore W4205500932C98274493 @default.
- W4205500932 hasConceptScore W4205500932C99631742 @default.
- W4205500932 hasIssue "117" @default.
- W4205500932 hasLocation W42055009321 @default.
- W4205500932 hasOpenAccess W4205500932 @default.
- W4205500932 hasPrimaryLocation W42055009321 @default.
- W4205500932 hasRelatedWork W2021185951 @default.
- W4205500932 hasRelatedWork W2028937236 @default.
- W4205500932 hasRelatedWork W2072955418 @default.
- W4205500932 hasRelatedWork W2131849270 @default.
- W4205500932 hasRelatedWork W2135913045 @default.
- W4205500932 hasRelatedWork W2556587095 @default.
- W4205500932 hasRelatedWork W2744352683 @default.
- W4205500932 hasRelatedWork W3168860169 @default.
- W4205500932 hasRelatedWork W3177286069 @default.
- W4205500932 hasRelatedWork W4283362117 @default.
- W4205500932 isParatext "false" @default.
- W4205500932 isRetracted "false" @default.
- W4205500932 workType "article" @default.