FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205533213> ?p ?o ?g. }

• W4205533213 endingPage "369" @default.
• W4205533213 startingPage "357" @default.
• W4205533213 abstract "Raddeanin A (RA) has indicated suppressive effects on various human tumor cells, and insufficient vitamin D was associated with human papillomavirus (HPV) persistence and gynecological tumors. However, combined effects of RA and vitamin D on HPV-positive cells remain elusive. Herein, we aimed to investigate the combined effects of RA and 1ɑ,25(OH)2D3 (VD3) on cellular viability and modulation of HPV18E6/E7, programmed cell death 1 ligand (PD-L1) and vitamin D receptor (VDR) expression in HeLa cells in vitro. HeLa cells were treated with RA alone or VD3 combined with RA. Cell viability was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and apoptosis was detected by flow cytometry. Real-time PCR (qRT-PCR) and Western blot were used to determine the gene/protein expression levels. The autophagosomes were observed by Transmission electron microscopy (TEM). The result showed that cell viability was inhibited by RA, and apoptosis in HeLa cells treated with RA was elevated accordingly. The expression of Bax, Cleaved-caspase-3, Cleaved-caspase-9 and Cleaved-PARP increased, and Bcl-2 decreased. The autophagy was induced by RA, as evidenced by elevated autophagosomes and the increased LC3-II/I ratio and Beclin-1. The expression of HPV18E6/E7, PD-L1 and VDR was reduced by RA. Moreover, RA combined with VD3 had a stronger effect on HeLa cells than RA alone. In conclusion, RA inhibits HeLa proliferation and induces apoptosis and autophagy via suppressing HPV18E6/E7, PD-L1 and VDR, and VD3 showed reinforced effects of RA on HeLa cells. Therefore, combined usage of VD3 with RA might be a potential novel immunotherapy strategy for HPV-related diseases." @default.
• W4205533213 created "2022-01-26" @default.
• W4205533213 creator A5004074027 @default.
• W4205533213 creator A5013017488 @default.
• W4205533213 creator A5037753129 @default.
• W4205533213 creator A5041748603 @default.
• W4205533213 creator A5043585983 @default.
• W4205533213 creator A5059218280 @default.
• W4205533213 creator A5066112101 @default.
• W4205533213 creator A5066255916 @default.
• W4205533213 creator A5088891615 @default.
• W4205533213 date "2022-01-01" @default.
• W4205533213 modified "2023-09-26" @default.
• W4205533213 title "1α,25(OH)₂D₃(VD3) promotes Raddeanin A-induced anti-proliferative effects on HeLa cell apoptosis and autophagy through negative regulation of HPV18E6-E7/PD-L1/VDR axis" @default.
• W4205533213 cites W1579431979 @default.
• W4205533213 cites W1983934585 @default.
• W4205533213 cites W1987364515 @default.
• W4205533213 cites W1994682335 @default.
• W4205533213 cites W2036186003 @default.
• W4205533213 cites W2083445131 @default.
• W4205533213 cites W2090395089 @default.
• W4205533213 cites W2114768673 @default.
• W4205533213 cites W2125610331 @default.
• W4205533213 cites W2146505724 @default.
• W4205533213 cites W2282604426 @default.
• W4205533213 cites W2359382339 @default.
• W4205533213 cites W2565956677 @default.
• W4205533213 cites W2570917027 @default.
• W4205533213 cites W2592067246 @default.
• W4205533213 cites W2740618823 @default.
• W4205533213 cites W2741366635 @default.
• W4205533213 cites W2744318356 @default.
• W4205533213 cites W2765785177 @default.
• W4205533213 cites W2774989997 @default.
• W4205533213 cites W2790455265 @default.
• W4205533213 cites W2890158083 @default.
• W4205533213 cites W2901234202 @default.
• W4205533213 cites W2912022807 @default.
• W4205533213 cites W2915737634 @default.
• W4205533213 cites W2919729384 @default.
• W4205533213 cites W2921473695 @default.
• W4205533213 cites W2922113472 @default.
• W4205533213 cites W2945869247 @default.
• W4205533213 cites W2948680670 @default.
• W4205533213 cites W2957636649 @default.
• W4205533213 cites W2966158307 @default.
• W4205533213 cites W2982312400 @default.
• W4205533213 cites W2991450217 @default.
• W4205533213 cites W2996268147 @default.
• W4205533213 cites W2998324388 @default.
• W4205533213 cites W2999358931 @default.
• W4205533213 cites W3003807889 @default.
• W4205533213 cites W3003811366 @default.
• W4205533213 cites W3006912435 @default.
• W4205533213 cites W3007651913 @default.
• W4205533213 cites W3007976024 @default.
• W4205533213 cites W3012503101 @default.
• W4205533213 cites W3015178699 @default.
• W4205533213 cites W3028256774 @default.
• W4205533213 cites W3045960922 @default.
• W4205533213 cites W3130808946 @default.
• W4205533213 cites W3136373236 @default.
• W4205533213 cites W3164819888 @default.
• W4205533213 doi "https://doi.org/10.1080/21655979.2021.2005223" @default.
• W4205533213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34974811" @default.
• W4205533213 hasPublicationYear "2022" @default.
• W4205533213 type Work @default.
• W4205533213 citedByCount "1" @default.
• W4205533213 countsByYear W42055332132022 @default.
• W4205533213 crossrefType "journal-article" @default.
• W4205533213 hasAuthorship W4205533213A5004074027 @default.
• W4205533213 hasAuthorship W4205533213A5013017488 @default.
• W4205533213 hasAuthorship W4205533213A5037753129 @default.
• W4205533213 hasAuthorship W4205533213A5041748603 @default.
• W4205533213 hasAuthorship W4205533213A5043585983 @default.
• W4205533213 hasAuthorship W4205533213A5059218280 @default.
• W4205533213 hasAuthorship W4205533213A5066112101 @default.
• W4205533213 hasAuthorship W4205533213A5066255916 @default.
• W4205533213 hasAuthorship W4205533213A5088891615 @default.
• W4205533213 hasBestOaLocation W42055332131 @default.
• W4205533213 hasConcept C104317684 @default.
• W4205533213 hasConcept C1491633281 @default.
• W4205533213 hasConcept C153911025 @default.
• W4205533213 hasConcept C170493617 @default.
• W4205533213 hasConcept C179639408 @default.
• W4205533213 hasConcept C185592680 @default.
• W4205533213 hasConcept C190283241 @default.
• W4205533213 hasConcept C203522944 @default.
• W4205533213 hasConcept C2776415932 @default.
• W4205533213 hasConcept C2777366897 @default.
• W4205533213 hasConcept C2780783641 @default.
• W4205533213 hasConcept C31573885 @default.
• W4205533213 hasConcept C502942594 @default.
• W4205533213 hasConcept C53227056 @default.
• W4205533213 hasConcept C553184892 @default.
• W4205533213 hasConcept C55493867 @default.
• W4205533213 hasConcept C62112901 @default.
• W4205533213 hasConcept C86803240 @default.

• next