Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205773975> ?p ?o ?g. }
- W4205773975 endingPage "51" @default.
- W4205773975 startingPage "42" @default.
- W4205773975 abstract "In this study, a novel high-purity polyethylene terephthalate (PET) hydrolase purification method was suggested, which was based on a simple heating protocol. To test the applicability of the method, the heating purification performances of different types of thermo-resistant PET hydrolases, including ThcCut1, ThcCut2, TfCut2, TfCut2-G62A, TfCut2-G62A/F209A, and LCC-F243I/D238C/S283C/Y127G, from crude extract were conducted. During the process, the effect of protectants and treatment duration on proteins and activity recoveries were tested. Results indicated that the specific activities of PET hydrolases purified by heating were generally as competitive as the ones purified by conventional nickel column methods, with higher protein recovery and enzyme activity recovery. The practical application of the heating purified PET hydrolases was further evaluated. Except for ThcCut2, other PET hydrolases exhibited better degradation performances of PET nanoparticles. The novel heating purification method for PET hydrolases showed good applicability, which can be extended to purify other thermo-resistant enzymes." @default.
- W4205773975 created "2022-01-26" @default.
- W4205773975 creator A5002481882 @default.
- W4205773975 creator A5016774012 @default.
- W4205773975 creator A5039109326 @default.
- W4205773975 creator A5041809750 @default.
- W4205773975 creator A5089929741 @default.
- W4205773975 date "2022-03-01" @default.
- W4205773975 modified "2023-10-18" @default.
- W4205773975 title "Purification of high-temperature resistant polyethylene terephthalate (PET) hydrolase by simple heating protocol" @default.
- W4205773975 cites W1966126307 @default.
- W4205773975 cites W1976183189 @default.
- W4205773975 cites W1978788492 @default.
- W4205773975 cites W1984158048 @default.
- W4205773975 cites W1991263269 @default.
- W4205773975 cites W1998315492 @default.
- W4205773975 cites W2003387643 @default.
- W4205773975 cites W2004653922 @default.
- W4205773975 cites W2009403319 @default.
- W4205773975 cites W2012168659 @default.
- W4205773975 cites W2014302049 @default.
- W4205773975 cites W2016405093 @default.
- W4205773975 cites W2023866307 @default.
- W4205773975 cites W2025443232 @default.
- W4205773975 cites W2048023725 @default.
- W4205773975 cites W2069104726 @default.
- W4205773975 cites W2076452812 @default.
- W4205773975 cites W2087693224 @default.
- W4205773975 cites W2088261910 @default.
- W4205773975 cites W2090111896 @default.
- W4205773975 cites W2121859449 @default.
- W4205773975 cites W2138635034 @default.
- W4205773975 cites W2144551578 @default.
- W4205773975 cites W2169545276 @default.
- W4205773975 cites W2175984513 @default.
- W4205773975 cites W2294707565 @default.
- W4205773975 cites W2302446488 @default.
- W4205773975 cites W2317258726 @default.
- W4205773975 cites W2331648056 @default.
- W4205773975 cites W2340916177 @default.
- W4205773975 cites W2400947447 @default.
- W4205773975 cites W2498068055 @default.
- W4205773975 cites W2498877559 @default.
- W4205773975 cites W2509464952 @default.
- W4205773975 cites W2565356656 @default.
- W4205773975 cites W2591719820 @default.
- W4205773975 cites W2600922952 @default.
- W4205773975 cites W2604230620 @default.
- W4205773975 cites W2610330214 @default.
- W4205773975 cites W2749156271 @default.
- W4205773975 cites W2790118122 @default.
- W4205773975 cites W2799993223 @default.
- W4205773975 cites W2801863874 @default.
- W4205773975 cites W2811130011 @default.
- W4205773975 cites W2893885963 @default.
- W4205773975 cites W2902841562 @default.
- W4205773975 cites W2903062369 @default.
- W4205773975 cites W2935008047 @default.
- W4205773975 cites W2937126532 @default.
- W4205773975 cites W2939625186 @default.
- W4205773975 cites W2946695027 @default.
- W4205773975 cites W2985553155 @default.
- W4205773975 cites W2998569228 @default.
- W4205773975 cites W2999239324 @default.
- W4205773975 cites W3003355911 @default.
- W4205773975 cites W3015207233 @default.
- W4205773975 cites W3027414047 @default.
- W4205773975 cites W4293247451 @default.
- W4205773975 cites W771021118 @default.
- W4205773975 doi "https://doi.org/10.1016/j.procbio.2022.01.011" @default.
- W4205773975 hasPublicationYear "2022" @default.
- W4205773975 type Work @default.
- W4205773975 citedByCount "2" @default.
- W4205773975 countsByYear W42057739752022 @default.
- W4205773975 countsByYear W42057739752023 @default.
- W4205773975 crossrefType "journal-article" @default.
- W4205773975 hasAuthorship W4205773975A5002481882 @default.
- W4205773975 hasAuthorship W4205773975A5016774012 @default.
- W4205773975 hasAuthorship W4205773975A5039109326 @default.
- W4205773975 hasAuthorship W4205773975A5041809750 @default.
- W4205773975 hasAuthorship W4205773975A5089929741 @default.
- W4205773975 hasConcept C159985019 @default.
- W4205773975 hasConcept C181199279 @default.
- W4205773975 hasConcept C185592680 @default.
- W4205773975 hasConcept C192562407 @default.
- W4205773975 hasConcept C198684833 @default.
- W4205773975 hasConcept C2780017307 @default.
- W4205773975 hasConcept C2780120296 @default.
- W4205773975 hasConcept C43617362 @default.
- W4205773975 hasConcept C55493867 @default.
- W4205773975 hasConceptScore W4205773975C159985019 @default.
- W4205773975 hasConceptScore W4205773975C181199279 @default.
- W4205773975 hasConceptScore W4205773975C185592680 @default.
- W4205773975 hasConceptScore W4205773975C192562407 @default.
- W4205773975 hasConceptScore W4205773975C198684833 @default.
- W4205773975 hasConceptScore W4205773975C2780017307 @default.
- W4205773975 hasConceptScore W4205773975C2780120296 @default.
- W4205773975 hasConceptScore W4205773975C43617362 @default.