Matches in SemOpenAlex for { <https://semopenalex.org/work/W4205786442> ?p ?o ?g. }
- W4205786442 endingPage "113985" @default.
- W4205786442 startingPage "113985" @default.
- W4205786442 abstract "Ryanodine receptors (RyR) located on the membrane of the endoplasmic reticulum (ER), are a potent regulator of intracellular calcium levels upon activation. Dysregulated Ca2+ homeostasis is characteristic of hypoxic-ischemic (HI) brain injury and ultimately leads to neurodegeneration. RyRs have thereby been implicated in the Ca2+ imbalance that occurs during and after HI. In this study, we investigated the effects of RyR antagonist, dantrolene, on HI brain injury in neonatal mice. We found that administration of dantrolene (i.p.) on postnatal day 7 mice reduced the infarction volume and morphological damage induced by HI, and improved functional recovery as assessed by neurobehavioral testing. The neuroprotective effect of dantrolene was further demonstrated in neuronal cell culture in vitro, where dantrolene significantly reduced oxygen-glucose deprivation (OGD)-induced cell death. Fura-2 calcium imaging confirmed that dantrolene reduced the intracellular calcium level in cultured cortical neurons in vitro. Finally, Western blot analysis showed that dantrolene treatment reduced cleaved caspase-3 and -9 apoptotic proteins, and elevated pro-survival protein kinase C (PKC) protein levels. Taken together, our results demonstrate that dantrolene exerts neuroprotective effects against neonatal HI brain injury. This suggests that RyRs play a role in mediating the ionic imbalance induced by HI and therefore represent a potential target for drug development." @default.
- W4205786442 created "2022-01-26" @default.
- W4205786442 creator A5002165527 @default.
- W4205786442 creator A5026142124 @default.
- W4205786442 creator A5033906893 @default.
- W4205786442 creator A5049618278 @default.
- W4205786442 creator A5054420556 @default.
- W4205786442 creator A5058158172 @default.
- W4205786442 creator A5059641847 @default.
- W4205786442 creator A5068918642 @default.
- W4205786442 creator A5071323598 @default.
- W4205786442 creator A5081340388 @default.
- W4205786442 creator A5087364900 @default.
- W4205786442 date "2022-05-01" @default.
- W4205786442 modified "2023-10-14" @default.
- W4205786442 title "Ryanodine receptor inhibitor dantrolene reduces hypoxic-ischemic brain injury in neonatal mice" @default.
- W4205786442 cites W14142049 @default.
- W4205786442 cites W1482301804 @default.
- W4205786442 cites W1486333767 @default.
- W4205786442 cites W1508909273 @default.
- W4205786442 cites W1529262908 @default.
- W4205786442 cites W1541024168 @default.
- W4205786442 cites W1586442162 @default.
- W4205786442 cites W1817435966 @default.
- W4205786442 cites W1950983788 @default.
- W4205786442 cites W1966513873 @default.
- W4205786442 cites W1967185557 @default.
- W4205786442 cites W1970275243 @default.
- W4205786442 cites W1972343960 @default.
- W4205786442 cites W1973688847 @default.
- W4205786442 cites W1978714931 @default.
- W4205786442 cites W1979370459 @default.
- W4205786442 cites W1983968962 @default.
- W4205786442 cites W1986662591 @default.
- W4205786442 cites W1988171628 @default.
- W4205786442 cites W1988312440 @default.
- W4205786442 cites W1992177920 @default.
- W4205786442 cites W1994056283 @default.
- W4205786442 cites W1994796214 @default.
- W4205786442 cites W2001229756 @default.
- W4205786442 cites W2003939380 @default.
- W4205786442 cites W2013815619 @default.
- W4205786442 cites W2013961675 @default.
- W4205786442 cites W2015912670 @default.
- W4205786442 cites W2017597607 @default.
- W4205786442 cites W2017832292 @default.
- W4205786442 cites W2019999960 @default.
- W4205786442 cites W2020577322 @default.
- W4205786442 cites W2025695281 @default.
- W4205786442 cites W2026849735 @default.
- W4205786442 cites W2028638185 @default.
- W4205786442 cites W2029643611 @default.
- W4205786442 cites W2033448044 @default.
- W4205786442 cites W2036158708 @default.
- W4205786442 cites W2036272059 @default.
- W4205786442 cites W2041024256 @default.
- W4205786442 cites W2042076314 @default.
- W4205786442 cites W2043572653 @default.
- W4205786442 cites W2046637240 @default.
- W4205786442 cites W2052857239 @default.
- W4205786442 cites W2058756209 @default.
- W4205786442 cites W2061610042 @default.
- W4205786442 cites W2062124189 @default.
- W4205786442 cites W2063541896 @default.
- W4205786442 cites W2064950130 @default.
- W4205786442 cites W2065340657 @default.
- W4205786442 cites W2066310239 @default.
- W4205786442 cites W2066742589 @default.
- W4205786442 cites W2066789184 @default.
- W4205786442 cites W2071928342 @default.
- W4205786442 cites W2072847038 @default.
- W4205786442 cites W2074194694 @default.
- W4205786442 cites W2074839068 @default.
- W4205786442 cites W2077021234 @default.
- W4205786442 cites W2078394930 @default.
- W4205786442 cites W2078504328 @default.
- W4205786442 cites W2079775458 @default.
- W4205786442 cites W2080309197 @default.
- W4205786442 cites W2085024380 @default.
- W4205786442 cites W2090180519 @default.
- W4205786442 cites W2096827547 @default.
- W4205786442 cites W2098597348 @default.
- W4205786442 cites W2103776949 @default.
- W4205786442 cites W2107601804 @default.
- W4205786442 cites W2109460976 @default.
- W4205786442 cites W2110909720 @default.
- W4205786442 cites W2111052126 @default.
- W4205786442 cites W2113596645 @default.
- W4205786442 cites W2115933592 @default.
- W4205786442 cites W2116635624 @default.
- W4205786442 cites W2120736516 @default.
- W4205786442 cites W2134076892 @default.
- W4205786442 cites W2136967026 @default.
- W4205786442 cites W2137141016 @default.
- W4205786442 cites W2137938185 @default.
- W4205786442 cites W2138009056 @default.
- W4205786442 cites W2140030356 @default.
- W4205786442 cites W2140605096 @default.