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- W4205823550 abstract "Changes in the intracellular concentration of free calcium ([Ca(2+)]i) regulate diverse cellular processes including fertilization. In mammalian eggs, the [Ca(2+)]i changes induced by the sperm unfold in a pattern of periodical rises, also known as [Ca(2+)]i oscillations. The source of Ca(2+) during oscillations is the endoplasmic reticulum ([Ca(2+)]ER), but it is presently unknown how [Ca(2+)]ER is regulated. Here, we show using mouse eggs that [Ca(2+)]i oscillations induced by a variety of agonists, including PLCζ, SrCl2 and thimerosal, provoke simultaneous but opposite changes in [Ca(2+)]ER and cause differential effects on the refilling and overall load of [Ca(2+)]ER. We also found that Ca(2+) influx is required to refill [Ca(2+)]ER, because the loss of [Ca(2+)]ER was accelerated in medium devoid of Ca(2+). Pharmacological inactivation of the function of the mitochondria and of the Ca(2+)-ATPase pumps PMCA and SERCA altered the pattern of oscillations and abruptly reduced [Ca(2+)]ER, especially after inactivation of mitochondria and SERCA functions. We also examined the expression of SERCA2b protein and found that it was expressed throughout oocyte maturation and attained a conspicuous cortical cluster organization in mature eggs. We show that its overexpression reduces the duration of inositol-1,4,5-trisphosphate-induced [Ca(2+)]i rises, promotes initiation of oscillations and enhances refilling of [Ca(2+)]ER. Collectively, our results provide novel insights on the regulation of [Ca(2+)]ER oscillations, which underlie the unique Ca(2+)-signalling system that activates the developmental program in mammalian eggs. PMID: 24101727 Funding information This work was supported by: NICHD NIH HHS, United States Grant ID: HD051872 NICHD NIH HHS, United States Grant ID: R01 HD051872" @default.
- W4205823550 created "2022-01-25" @default.
- W4205823550 creator A5024729774 @default.
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- W4205823550 date "2014-03-04" @default.
- W4205823550 modified "2023-09-27" @default.
- W4205823550 title "Faculty Opinions recommendation of Regulation of endoplasmic reticulum Ca(2+) oscillations in mammalian eggs." @default.
- W4205823550 doi "https://doi.org/10.3410/f.718135732.793491636" @default.
- W4205823550 hasPublicationYear "2014" @default.
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