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- W4205966088 abstract "Triple-negative breast cancer (TNBC) is a breast cancer (BC) subtype that accounts for approximately 15-20% of all BC cases. Cancer cell lines (CLs) provide an efficient way to model the disease. We have recently isolated a patient-derived triple-negative BC CL MFUM-BrTNBC-1 and performed a detailed morphological and molecular characterisation and a comprehensive comparison with three commercial BC CLs (MCF-7, MDA-MB-231, MDA-MB-453). Light and fluorescence microscopy were used for morphological studies; immunocytochemical staining for hormone receptor, p53 and Ki67 status; RNA sequencing, qRT-PCR and STR analysis for molecular characterisation; and biomedical image analysis for comparative phenotypical analysis. The patient tissue-derived MFUM-BrTNBC-1 maintained the primary triple-negative receptor status. STR analysis showed a stable and unique STR profile up to the 6th passage. MFUM-BrTNBC-1 expressed EMT transition markers and displayed changes in several cancer-related pathways (MAPK, Wnt and PI3K signalling; nucleotide excision repair; and SWI/SNF chromatin remodelling). Morphologically, MFUM-BrTNBC-1 differed from the commercial TNBC CL MDA-MB-231. The advantages of MFUM-BrTNBC-1 are its isolation from a primary tumour, rather than a metastatic site; good growth characteristics; phenotype identical to primary tissue; complete records of origin; a unique identifier; complete, unique STR profile; quantifiable morphological properties; and genetic stability up to (at least) the 6th passage." @default.
- W4205966088 created "2022-01-25" @default.
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- W4205966088 date "2021-12-30" @default.
- W4205966088 modified "2023-10-16" @default.
- W4205966088 title "MFUM-BrTNBC-1, a Newly Established Patient-Derived Triple-Negative Breast Cancer Cell Line: Molecular Characterisation, Genetic Stability, and Comprehensive Comparison with Commercial Breast Cancer Cell Lines" @default.
- W4205966088 cites W1555009525 @default.
- W4205966088 cites W1612068869 @default.
- W4205966088 cites W1630763239 @default.
- W4205966088 cites W168811426 @default.
- W4205966088 cites W1827425370 @default.
- W4205966088 cites W1872860230 @default.
- W4205966088 cites W1890809256 @default.
- W4205966088 cites W1964949716 @default.
- W4205966088 cites W1965267351 @default.
- W4205966088 cites W1965659632 @default.
- W4205966088 cites W1966542709 @default.
- W4205966088 cites W1967293289 @default.
- W4205966088 cites W1968051733 @default.
- W4205966088 cites W1971146759 @default.
- W4205966088 cites W1986694287 @default.
- W4205966088 cites W1986911605 @default.
- W4205966088 cites W1988941884 @default.
- W4205966088 cites W1993738751 @default.
- W4205966088 cites W1994930469 @default.
- W4205966088 cites W2015505424 @default.
- W4205966088 cites W2029018044 @default.
- W4205966088 cites W2036673057 @default.
- W4205966088 cites W2040092183 @default.
- W4205966088 cites W2042316019 @default.
- W4205966088 cites W2043398720 @default.
- W4205966088 cites W2043787658 @default.
- W4205966088 cites W2047578120 @default.
- W4205966088 cites W2062814183 @default.
- W4205966088 cites W2068875547 @default.
- W4205966088 cites W2069793009 @default.
- W4205966088 cites W2071906362 @default.
- W4205966088 cites W2082996759 @default.
- W4205966088 cites W2086965865 @default.
- W4205966088 cites W2088484810 @default.
- W4205966088 cites W2094627540 @default.
- W4205966088 cites W2098740402 @default.
- W4205966088 cites W2103179773 @default.
- W4205966088 cites W2103885797 @default.
- W4205966088 cites W2109076411 @default.
- W4205966088 cites W2114570899 @default.
- W4205966088 cites W2114941442 @default.
- W4205966088 cites W2118992835 @default.
- W4205966088 cites W2129470488 @default.
- W4205966088 cites W2131554707 @default.
- W4205966088 cites W2134539328 @default.
- W4205966088 cites W2135405268 @default.
- W4205966088 cites W2148678536 @default.
- W4205966088 cites W2150134401 @default.
- W4205966088 cites W2169456326 @default.
- W4205966088 cites W2208636896 @default.
- W4205966088 cites W2227972917 @default.
- W4205966088 cites W2255395527 @default.
- W4205966088 cites W2264655007 @default.
- W4205966088 cites W2268122434 @default.
- W4205966088 cites W2294754125 @default.
- W4205966088 cites W2529945511 @default.
- W4205966088 cites W2599572618 @default.
- W4205966088 cites W2600418040 @default.
- W4205966088 cites W2608202676 @default.
- W4205966088 cites W2727917467 @default.
- W4205966088 cites W2751860027 @default.
- W4205966088 cites W2756562465 @default.
- W4205966088 cites W2766084386 @default.
- W4205966088 cites W2770095154 @default.
- W4205966088 cites W2770776365 @default.
- W4205966088 cites W2789416018 @default.
- W4205966088 cites W2793707558 @default.
- W4205966088 cites W2799669931 @default.
- W4205966088 cites W2802776623 @default.
- W4205966088 cites W2805978167 @default.
- W4205966088 cites W2886485146 @default.
- W4205966088 cites W2886855629 @default.
- W4205966088 cites W2889250103 @default.
- W4205966088 cites W2901068426 @default.
- W4205966088 cites W2901218215 @default.
- W4205966088 cites W2904835884 @default.
- W4205966088 cites W2912993657 @default.
- W4205966088 cites W2924773386 @default.
- W4205966088 cites W2951429562 @default.
- W4205966088 cites W2954178256 @default.
- W4205966088 cites W2965206386 @default.
- W4205966088 cites W2965221998 @default.