FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206109316> ?p ?o ?g. }

• W4206109316 abstract "Industrial fermentation processes strive for high robustness to ensure optimal and consistent performance. Medium components, fermentation products, and physical perturbations may cause stress and lower performance. Cellular stress elicits a range of responses, whose extracellular manifestations have been extensively studied; whereas intracellular aspects remain poorly known due to lack of tools for real-time monitoring. Genetically encoded biosensors have emerged as promising tools and have been used to improve microbial productivity and tolerance toward industrially relevant stresses. Here, fluorescent biosensors able to sense the yeast intracellular environment (pH, ATP levels, oxidative stress, glycolytic flux, and ribosome production) were implemented into a versatile and easy-to-use toolbox. Marker-free and efficient genome integration at a conserved site on chromosome X of Saccharomyces cerevisiae strains and a commercial Saccharomyces boulardii strain was developed. Moreover, multiple biosensors were used to simultaneously monitor different intracellular parameters in a single cell. Even when combined together, the biosensors did not significantly affect key physiological parameters, such as specific growth rate and product yields. Activation and response of each biosensor and their interconnection were assessed using an advanced micro-cultivation system. Finally, the toolbox was used to screen cell behavior in a synthetic lignocellulosic hydrolysate that mimicked harsh industrial substrates, revealing differences in the oxidative stress response between laboratory (CEN.PK113-7D) and industrial (Ethanol Red) S. cerevisiae strains. In summary, the toolbox will allow both the exploration of yeast diversity and physiological responses in natural and complex industrial conditions, as well as the possibility to monitor production processes." @default.
• W4206109316 created "2022-01-25" @default.
• W4206109316 creator A5034405101 @default.
• W4206109316 creator A5054654352 @default.
• W4206109316 creator A5064436880 @default.
• W4206109316 date "2022-01-07" @default.
• W4206109316 modified "2023-10-16" @default.
• W4206109316 title "Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors" @default.
• W4206109316 cites W1195052857 @default.
• W4206109316 cites W1537939025 @default.
• W4206109316 cites W1598771428 @default.
• W4206109316 cites W1940678034 @default.
• W4206109316 cites W1968101639 @default.
• W4206109316 cites W1968806949 @default.
• W4206109316 cites W1970351604 @default.
• W4206109316 cites W1975952108 @default.
• W4206109316 cites W1988992269 @default.
• W4206109316 cites W1997184942 @default.
• W4206109316 cites W2006085890 @default.
• W4206109316 cites W2014133014 @default.
• W4206109316 cites W2035980207 @default.
• W4206109316 cites W2040105137 @default.
• W4206109316 cites W2081425013 @default.
• W4206109316 cites W2082995554 @default.
• W4206109316 cites W2102950470 @default.
• W4206109316 cites W2107910846 @default.
• W4206109316 cites W2109171263 @default.
• W4206109316 cites W2113206920 @default.
• W4206109316 cites W2113393062 @default.
• W4206109316 cites W2117924928 @default.
• W4206109316 cites W2118829612 @default.
• W4206109316 cites W2121966499 @default.
• W4206109316 cites W2123724487 @default.
• W4206109316 cites W2128828121 @default.
• W4206109316 cites W2129739437 @default.
• W4206109316 cites W2131843496 @default.
• W4206109316 cites W2139006758 @default.
• W4206109316 cites W2141632111 @default.
• W4206109316 cites W2144036514 @default.
• W4206109316 cites W2149503748 @default.
• W4206109316 cites W2156929072 @default.
• W4206109316 cites W2157009707 @default.
• W4206109316 cites W2157163471 @default.
• W4206109316 cites W2160718564 @default.
• W4206109316 cites W2165646201 @default.
• W4206109316 cites W2165870010 @default.
• W4206109316 cites W2172296079 @default.
• W4206109316 cites W2231562389 @default.
• W4206109316 cites W2295025783 @default.
• W4206109316 cites W2435084741 @default.
• W4206109316 cites W2471393621 @default.
• W4206109316 cites W2557455152 @default.
• W4206109316 cites W2568012904 @default.
• W4206109316 cites W2592137191 @default.
• W4206109316 cites W2595700476 @default.
• W4206109316 cites W2606227689 @default.
• W4206109316 cites W2611277106 @default.
• W4206109316 cites W2620930341 @default.
• W4206109316 cites W2781193336 @default.
• W4206109316 cites W2793282492 @default.
• W4206109316 cites W2794379535 @default.
• W4206109316 cites W2798086243 @default.
• W4206109316 cites W2802214715 @default.
• W4206109316 cites W2803934794 @default.
• W4206109316 cites W2884156626 @default.
• W4206109316 cites W2886229797 @default.
• W4206109316 cites W2900206496 @default.
• W4206109316 cites W2915338207 @default.
• W4206109316 cites W2921521950 @default.
• W4206109316 cites W2945567049 @default.
• W4206109316 cites W2951421438 @default.
• W4206109316 cites W2966124235 @default.
• W4206109316 cites W2971595838 @default.
• W4206109316 cites W2974780560 @default.
• W4206109316 cites W2979679211 @default.
• W4206109316 cites W2979938773 @default.
• W4206109316 cites W3005536181 @default.
• W4206109316 cites W3005838565 @default.
• W4206109316 cites W3036804912 @default.
• W4206109316 cites W3037438223 @default.
• W4206109316 cites W3041896024 @default.
• W4206109316 cites W3046062799 @default.
• W4206109316 cites W3082072705 @default.
• W4206109316 cites W3086461899 @default.
• W4206109316 cites W3091775330 @default.
• W4206109316 cites W3096981838 @default.
• W4206109316 cites W3110986547 @default.
• W4206109316 cites W3122180593 @default.
• W4206109316 cites W3127221300 @default.
• W4206109316 cites W3156162838 @default.
• W4206109316 cites W3157648034 @default.
• W4206109316 cites W3160975074 @default.
• W4206109316 cites W3168413219 @default.
• W4206109316 cites W3197820211 @default.
• W4206109316 cites W4234751839 @default.
• W4206109316 cites W4251139549 @default.
• W4206109316 cites W70082989 @default.
• W4206109316 cites W99972145 @default.
• W4206109316 doi "https://doi.org/10.3389/fmicb.2021.802169" @default.
• W4206109316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35069506" @default.

• next