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• W4206111036 abstract "In the study of embryo development process, the morphological features at different stages are essential to evaluate developmental competence of the embryo, which can be used to optimize and improve the system for in-vitro embryo culture. In this paper, an online monitoring system was designed for long-term culture of embryos, based on a monitoring strategy of low-magnification search and high-magnification observation. Three optical modules of 4× phase contrast, 10× and 20× Hoffman modulation phase contrast were configured in this system to meet the requirements of different fields of view, especially when the size of the embryo increases during the culture. Using an optomechanical system matching design, an error control and alignment test, the resolution of optical imaging was guaranteed, and a relief stereoscopic imaging with high contrast of embryos was obtained. Through low-magnification field of view to identify and locate embryos and high-magnification field of view to capture the details, the system realized online tracking and monitoring of embryos. In addition, we developed and verified an embryo identifying and locating algorithm based on image contour area and definition evaluation. The online monitoring system of in-vitro embryo culture proposed in this paper can track and record the morphological features of embryos without affecting the embryo development, providing a basis for the assessment of embryo development and the optimization of in-vitro culture system.在胚胎发育过程的研究中，胚胎各阶段的形态特征是评价其发育优劣的重要依据，用于指导胚胎体外培养体系的优化和改进。本文为胚胎长时程培养设计了一种基于“低倍搜寻、高倍观察”策略的在线监测系统。首先，针对胚胎在发育过程中体积不断增大的情况，本系统配置了4倍相差、10倍和20倍霍夫曼调制相衬三种光学模块，以满足不同视场的观察需求；通过光机系统匹配设计、误差控制和装调试验，保证了成像光学分辨率，并获得了胚胎的高对比度的浮雕立体成像效果；然后，通过低倍大视场进行识别定位，高倍小视场捕获细节，本系统实现了胚胎的在线跟踪监测；最后，开发并验证了一种基于图像轮廓面积和清晰度评价的胚胎识别定位算法。本文开发的体外胚胎培养在线监测系统能在不影响胚胎发育的前提下，跟踪记录发育过程的形态特征，为胚胎发育评估和体外培养体系的优化提供依据。." @default.
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• W4206111036 date "2021-12-25" @default.
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• W4206111036 title "[Design and experiment of online monitoring system for long-term culture of embryo]." @default.
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• W4206111036 doi "https://doi.org/10.7507/1001-5515.202107053" @default.
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