Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206121640> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W4206121640 abstract "There is a 1-4 mmol/L rise in plasma sodium concentrations in individuals with high salt intake and in patients with essential hypertension. In this study, we used 3 independent assays to determine whether such a small increase in sodium concentrations per se alters endothelial nitric oxide synthase (eNOS) function and contributes to hypertension. By directly measuring NOS activity in living bovine aortic endothelial cells, we demonstrated that a 5-mmol/L increase in salt concentration (from 137 to 142 mmol/L) caused a 25% decrease in NOS activity. Importantly, the decrease in NOS activity was in a salt concentration-dependent manner. The NOS activity was decreased by 25, 45, and 70%, with the increase of 5, 10, and 20 mmol/L of NaCl, respectively. Using Chinese hamster ovary cells stably expressing eNOS, we confirmed the inhibitory effects of salt on eNOS activity. The eNOS activity was unaffected in the presence of equal milliosmol of mannitol, which excludes an osmotic effect. Using an ex vivo aortic angiogenesis assay, we demonstrated that salt attenuated the nitric oxide (NO)-dependent proliferation of endothelial cells. By directly monitoring blood pressure changes in response to salt infusion, we found that in vivo infusion of salt induced an acute increase in blood pressure in a salt concentration-dependent manner. In conclusion, our findings demonstrated that eNOS is sensitive to changes in salt concentration. A 5-mmol/L rise in salt concentration, within the range observed in essential hypertension patients or in individuals with high salt intake, could significantly suppress eNOS activity. This salt-induced reduction in NO generation in endothelial cells may contribute to the development of hypertension. PMID: 19176751 Funding information This work was supported by: NIGMS NIH HHS, United States Grant ID: R01GM085231 NIGMS NIH HHS, United States Grant ID: R01 GM085231-02 NIGMS NIH HHS, United States Grant ID: R01 GM085231-01 NIGMS NIH HHS, United States Grant ID: R01 GM085231 NCRR NIH HHS, United States Grant ID: 2P0RR015592" @default.
- W4206121640 created "2022-01-26" @default.
- W4206121640 creator A5017239585 @default.
- W4206121640 date "2012-09-12" @default.
- W4206121640 modified "2023-09-28" @default.
- W4206121640 title "Faculty Opinions recommendation of Salt inactivates endothelial nitric oxide synthase in endothelial cells." @default.
- W4206121640 doi "https://doi.org/10.3410/f.717955777.793460746" @default.
- W4206121640 hasPublicationYear "2012" @default.
- W4206121640 type Work @default.
- W4206121640 citedByCount "0" @default.
- W4206121640 crossrefType "dataset" @default.
- W4206121640 hasAuthorship W4206121640A5017239585 @default.
- W4206121640 hasBestOaLocation W42061216401 @default.
- W4206121640 hasConcept C126322002 @default.
- W4206121640 hasConcept C134018914 @default.
- W4206121640 hasConcept C150903083 @default.
- W4206121640 hasConcept C178790620 @default.
- W4206121640 hasConcept C185592680 @default.
- W4206121640 hasConcept C202751555 @default.
- W4206121640 hasConcept C207001950 @default.
- W4206121640 hasConcept C26291073 @default.
- W4206121640 hasConcept C2777622882 @default.
- W4206121640 hasConcept C2778326061 @default.
- W4206121640 hasConcept C2778445172 @default.
- W4206121640 hasConcept C2908688039 @default.
- W4206121640 hasConcept C519581460 @default.
- W4206121640 hasConcept C537181965 @default.
- W4206121640 hasConcept C55493867 @default.
- W4206121640 hasConcept C71924100 @default.
- W4206121640 hasConcept C86803240 @default.
- W4206121640 hasConceptScore W4206121640C126322002 @default.
- W4206121640 hasConceptScore W4206121640C134018914 @default.
- W4206121640 hasConceptScore W4206121640C150903083 @default.
- W4206121640 hasConceptScore W4206121640C178790620 @default.
- W4206121640 hasConceptScore W4206121640C185592680 @default.
- W4206121640 hasConceptScore W4206121640C202751555 @default.
- W4206121640 hasConceptScore W4206121640C207001950 @default.
- W4206121640 hasConceptScore W4206121640C26291073 @default.
- W4206121640 hasConceptScore W4206121640C2777622882 @default.
- W4206121640 hasConceptScore W4206121640C2778326061 @default.
- W4206121640 hasConceptScore W4206121640C2778445172 @default.
- W4206121640 hasConceptScore W4206121640C2908688039 @default.
- W4206121640 hasConceptScore W4206121640C519581460 @default.
- W4206121640 hasConceptScore W4206121640C537181965 @default.
- W4206121640 hasConceptScore W4206121640C55493867 @default.
- W4206121640 hasConceptScore W4206121640C71924100 @default.
- W4206121640 hasConceptScore W4206121640C86803240 @default.
- W4206121640 hasLocation W42061216401 @default.
- W4206121640 hasOpenAccess W4206121640 @default.
- W4206121640 hasPrimaryLocation W42061216401 @default.
- W4206121640 hasRelatedWork W102080802 @default.
- W4206121640 hasRelatedWork W1863987436 @default.
- W4206121640 hasRelatedWork W2061561342 @default.
- W4206121640 hasRelatedWork W2061658648 @default.
- W4206121640 hasRelatedWork W2085491702 @default.
- W4206121640 hasRelatedWork W2114204950 @default.
- W4206121640 hasRelatedWork W2148878791 @default.
- W4206121640 hasRelatedWork W2170387219 @default.
- W4206121640 hasRelatedWork W2395494434 @default.
- W4206121640 hasRelatedWork W170559647 @default.
- W4206121640 isParatext "false" @default.
- W4206121640 isRetracted "false" @default.
- W4206121640 workType "dataset" @default.