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- W4206183003 abstract "Fluorescence super-resolution (SR) microscopy has definitely advanced from the initial scepticism towards becoming the replacement of conventional microscopy. Indeed, thanks to several advancements in technologies (e.g., lasers, detectors, electronics, fluorescent probes) and new concepts (e.g., illumination strategies, labeling protocols, data analysis), SR microscopy is continuously closing the gap to electron microscopy in terms of spatial resolution, and, just as important, SR microscopy is becoming much gentler on living tissue samples. In this chapter, we briefly introduce fluorescence SR microscopy, followed by a discussion on conventional classification: wide-field-based vs laser-scanning-based techniques. We then focus only on the class of laser-scanning microscopy by describing their strategies to achieve sub-diffraction resolution. In particular, we analyze the pros and cons of confocal, image-scanning, stimulated emission depletion, and reversible saturable optical fluorescence transition microscopy, which currently represent the most mature laser-scanning-based SR techniques. We evaluate these techniques from a theoretical point of view and in terms of imaging of living biological systems. Finally, we envisage some perspectives." @default.
- W4206183003 created "2022-01-25" @default.
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- W4206183003 date "2021-12-14" @default.
- W4206183003 modified "2023-09-27" @default.
- W4206183003 title "Super-Resolution Imaging through Laser-Scanning Microscopy" @default.
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- W4206183003 doi "https://doi.org/10.1063/9780735423794_003" @default.
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