Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206184347> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W4206184347 abstract "Skeletal muscle contains multiple progenitor populations of distinct embryonic origins and developmental potential. Myogenic progenitors, usually residing in a satellite cell position between the myofiber plasma membrane and the laminin-rich basement membrane that ensheaths it, are self-renewing cells that are solely committed to the myogenic lineage1,2. We have recently described a second class of vessel associated progenitors that can generate myofibroblasts and white adipocytes, which responds to damage by efficiently entering proliferation and provides trophic support to myogenic cells during tissue regeneration3,4. One of the most trusted assays to determine the developmental and regenerative potential of a given cell population relies on their isolation and transplantation5-7. To this end we have optimized protocols for their purification by flow cytometry from enzymatically dissociated muscle, which we will outline in this article. The populations obtained using this method will contain either myogenic or fibro/adipogenic colony forming cells: no other cell types are capable of expanding in vitro or surviving in vivo delivery. However, when these populations are used immediately after the sort for molecular analysis (e.g qRT-PCR) one must keep in mind that the freshly sorted subsets may contain other contaminant cells that lack the ability of forming colonies or engrafting recipients." @default.
- W4206184347 created "2022-01-26" @default.
- W4206184347 creator A5052991360 @default.
- W4206184347 creator A5062416994 @default.
- W4206184347 date "2011-03-16" @default.
- W4206184347 modified "2023-10-16" @default.
- W4206184347 title "JoVE Video Dataset" @default.
- W4206184347 doi "https://doi.org/10.3791/2476-v" @default.
- W4206184347 hasPublicationYear "2011" @default.
- W4206184347 type Work @default.
- W4206184347 citedByCount "0" @default.
- W4206184347 crossrefType "journal-article" @default.
- W4206184347 hasAuthorship W4206184347A5052991360 @default.
- W4206184347 hasAuthorship W4206184347A5062416994 @default.
- W4206184347 hasConcept C104317684 @default.
- W4206184347 hasConcept C144024400 @default.
- W4206184347 hasConcept C145103041 @default.
- W4206184347 hasConcept C1491633281 @default.
- W4206184347 hasConcept C149923435 @default.
- W4206184347 hasConcept C189165786 @default.
- W4206184347 hasConcept C201750760 @default.
- W4206184347 hasConcept C203014093 @default.
- W4206184347 hasConcept C207200792 @default.
- W4206184347 hasConcept C2776193487 @default.
- W4206184347 hasConcept C2778630268 @default.
- W4206184347 hasConcept C2779814568 @default.
- W4206184347 hasConcept C28328180 @default.
- W4206184347 hasConcept C2908647359 @default.
- W4206184347 hasConcept C3763915 @default.
- W4206184347 hasConcept C54355233 @default.
- W4206184347 hasConcept C553184892 @default.
- W4206184347 hasConcept C86803240 @default.
- W4206184347 hasConcept C95444343 @default.
- W4206184347 hasConceptScore W4206184347C104317684 @default.
- W4206184347 hasConceptScore W4206184347C144024400 @default.
- W4206184347 hasConceptScore W4206184347C145103041 @default.
- W4206184347 hasConceptScore W4206184347C1491633281 @default.
- W4206184347 hasConceptScore W4206184347C149923435 @default.
- W4206184347 hasConceptScore W4206184347C189165786 @default.
- W4206184347 hasConceptScore W4206184347C201750760 @default.
- W4206184347 hasConceptScore W4206184347C203014093 @default.
- W4206184347 hasConceptScore W4206184347C207200792 @default.
- W4206184347 hasConceptScore W4206184347C2776193487 @default.
- W4206184347 hasConceptScore W4206184347C2778630268 @default.
- W4206184347 hasConceptScore W4206184347C2779814568 @default.
- W4206184347 hasConceptScore W4206184347C28328180 @default.
- W4206184347 hasConceptScore W4206184347C2908647359 @default.
- W4206184347 hasConceptScore W4206184347C3763915 @default.
- W4206184347 hasConceptScore W4206184347C54355233 @default.
- W4206184347 hasConceptScore W4206184347C553184892 @default.
- W4206184347 hasConceptScore W4206184347C86803240 @default.
- W4206184347 hasConceptScore W4206184347C95444343 @default.
- W4206184347 hasIssue "49" @default.
- W4206184347 hasLocation W42061843471 @default.
- W4206184347 hasOpenAccess W4206184347 @default.
- W4206184347 hasPrimaryLocation W42061843471 @default.
- W4206184347 hasRelatedWork W1971286991 @default.
- W4206184347 hasRelatedWork W1984423229 @default.
- W4206184347 hasRelatedWork W2067061618 @default.
- W4206184347 hasRelatedWork W2071902795 @default.
- W4206184347 hasRelatedWork W2081331195 @default.
- W4206184347 hasRelatedWork W2115588505 @default.
- W4206184347 hasRelatedWork W2135339594 @default.
- W4206184347 hasRelatedWork W2171547034 @default.
- W4206184347 hasRelatedWork W3213450778 @default.
- W4206184347 hasRelatedWork W3590519 @default.
- W4206184347 isParatext "false" @default.
- W4206184347 isRetracted "false" @default.
- W4206184347 workType "article" @default.